Effects of low-dose exposure to pesticide mixture on physiological responses of the pacific oyster, Crassostrea gigas
This study investigated the effects on the physiology of Pacific oyster, Crassostrea gigas, of a mixture of pesticides containing 0.8 μg L−1 alachlor, 0.6 μg L−1 metolachlor, 0.7 μg L−1 atrazine, 0.6 μg L−1 terbuthylazine, 0.5 μg L−1 diuron, 0.6 μg L−1 fosetyl aluminum, 0.05 μg L−1 carbaryl, and 0.7...
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Online Access: | https://archimer.ifremer.fr/doc/00065/17641/88201.pdf https://doi.org/10.1002/tox.20764 https://archimer.ifremer.fr/doc/00065/17641/ |
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ftarchimer:oai:archimer.ifremer.fr:17641 2023-05-15T15:58:26+02:00 Effects of low-dose exposure to pesticide mixture on physiological responses of the pacific oyster, Crassostrea gigas Geret, Florence Burgeot, Thierry Haure, Joel Gagnaire, Beatrice Renault, Tristan Communal, Pierre-yves Samain, Jean-francois 2013-12 application/pdf https://archimer.ifremer.fr/doc/00065/17641/88201.pdf https://doi.org/10.1002/tox.20764 https://archimer.ifremer.fr/doc/00065/17641/ eng eng Wiley-blackwell https://archimer.ifremer.fr/doc/00065/17641/88201.pdf doi:10.1002/tox.20764 https://archimer.ifremer.fr/doc/00065/17641/ 2011 Wiley Periodicals, Inc. info:eu-repo/semantics/openAccess restricted use Environmental Toxicology (1520-4081) (Wiley-blackwell), 2013-12 , Vol. 28 , N. 12 , P. 689-699 pesticides oyster immunotoxicity catalase glutathione S-transferase glutamine synthetase DNA adducts text Publication info:eu-repo/semantics/article 2013 ftarchimer https://doi.org/10.1002/tox.20764 2021-09-23T20:20:20Z This study investigated the effects on the physiology of Pacific oyster, Crassostrea gigas, of a mixture of pesticides containing 0.8 μg L−1 alachlor, 0.6 μg L−1 metolachlor, 0.7 μg L−1 atrazine, 0.6 μg L−1 terbuthylazine, 0.5 μg L−1 diuron, 0.6 μg L−1 fosetyl aluminum, 0.05 μg L−1 carbaryl, and 0.7 μg L−1 glyphosate for a total concentration of 4.55 μg L−1. The total nominal concentration of pesticides mixture corresponds to the pesticide concentrations in the shellfish culture area of the Marennes-Oleron basin. Two varieties of C. gigas were selected on the foreshore, based on their characteristics in terms of resistance to summer mortality, to assess the effects of the pesticide mixture after 7 days of exposure under controlled conditions. The early effects of the mixture were assessed using enzyme biomarkers of nitrogen metabolism (GS, glutamine synthetase), detoxification metabolism (GST, glutathione S-transferase), and oxidative stress (CAT, catalase). Sublethal effects on hemocyte parameters (phagocytosis and esterase activity) and DNA damages (DNA adducts) were also measured. Changes in metabolic activities were characterized by increases in GS, GST, and CAT levels on the first day of exposure for the “resistant” oysters and after 3–7 days of exposure for the “susceptible” oysters. The formation of DNA adducts was detected after 7 days of exposure. The percentage of hemocyte esterase-positive cells was reduced in the resistant oysters, as was the hemocyte phagocytic capacity in both oyster varieties after 7 days of exposure to the pesticide mixture. This study highlights the need to consider the low doses and the mixture of pesticides to evaluate the effects of these molecules on organisms. © 2011 Wiley Periodicals, Inc. Environ Toxicol, 2011. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Pacific Environmental Toxicology 28 12 689 699 |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
pesticides oyster immunotoxicity catalase glutathione S-transferase glutamine synthetase DNA adducts |
spellingShingle |
pesticides oyster immunotoxicity catalase glutathione S-transferase glutamine synthetase DNA adducts Geret, Florence Burgeot, Thierry Haure, Joel Gagnaire, Beatrice Renault, Tristan Communal, Pierre-yves Samain, Jean-francois Effects of low-dose exposure to pesticide mixture on physiological responses of the pacific oyster, Crassostrea gigas |
topic_facet |
pesticides oyster immunotoxicity catalase glutathione S-transferase glutamine synthetase DNA adducts |
description |
This study investigated the effects on the physiology of Pacific oyster, Crassostrea gigas, of a mixture of pesticides containing 0.8 μg L−1 alachlor, 0.6 μg L−1 metolachlor, 0.7 μg L−1 atrazine, 0.6 μg L−1 terbuthylazine, 0.5 μg L−1 diuron, 0.6 μg L−1 fosetyl aluminum, 0.05 μg L−1 carbaryl, and 0.7 μg L−1 glyphosate for a total concentration of 4.55 μg L−1. The total nominal concentration of pesticides mixture corresponds to the pesticide concentrations in the shellfish culture area of the Marennes-Oleron basin. Two varieties of C. gigas were selected on the foreshore, based on their characteristics in terms of resistance to summer mortality, to assess the effects of the pesticide mixture after 7 days of exposure under controlled conditions. The early effects of the mixture were assessed using enzyme biomarkers of nitrogen metabolism (GS, glutamine synthetase), detoxification metabolism (GST, glutathione S-transferase), and oxidative stress (CAT, catalase). Sublethal effects on hemocyte parameters (phagocytosis and esterase activity) and DNA damages (DNA adducts) were also measured. Changes in metabolic activities were characterized by increases in GS, GST, and CAT levels on the first day of exposure for the “resistant” oysters and after 3–7 days of exposure for the “susceptible” oysters. The formation of DNA adducts was detected after 7 days of exposure. The percentage of hemocyte esterase-positive cells was reduced in the resistant oysters, as was the hemocyte phagocytic capacity in both oyster varieties after 7 days of exposure to the pesticide mixture. This study highlights the need to consider the low doses and the mixture of pesticides to evaluate the effects of these molecules on organisms. © 2011 Wiley Periodicals, Inc. Environ Toxicol, 2011. |
format |
Article in Journal/Newspaper |
author |
Geret, Florence Burgeot, Thierry Haure, Joel Gagnaire, Beatrice Renault, Tristan Communal, Pierre-yves Samain, Jean-francois |
author_facet |
Geret, Florence Burgeot, Thierry Haure, Joel Gagnaire, Beatrice Renault, Tristan Communal, Pierre-yves Samain, Jean-francois |
author_sort |
Geret, Florence |
title |
Effects of low-dose exposure to pesticide mixture on physiological responses of the pacific oyster, Crassostrea gigas |
title_short |
Effects of low-dose exposure to pesticide mixture on physiological responses of the pacific oyster, Crassostrea gigas |
title_full |
Effects of low-dose exposure to pesticide mixture on physiological responses of the pacific oyster, Crassostrea gigas |
title_fullStr |
Effects of low-dose exposure to pesticide mixture on physiological responses of the pacific oyster, Crassostrea gigas |
title_full_unstemmed |
Effects of low-dose exposure to pesticide mixture on physiological responses of the pacific oyster, Crassostrea gigas |
title_sort |
effects of low-dose exposure to pesticide mixture on physiological responses of the pacific oyster, crassostrea gigas |
publisher |
Wiley-blackwell |
publishDate |
2013 |
url |
https://archimer.ifremer.fr/doc/00065/17641/88201.pdf https://doi.org/10.1002/tox.20764 https://archimer.ifremer.fr/doc/00065/17641/ |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Environmental Toxicology (1520-4081) (Wiley-blackwell), 2013-12 , Vol. 28 , N. 12 , P. 689-699 |
op_relation |
https://archimer.ifremer.fr/doc/00065/17641/88201.pdf doi:10.1002/tox.20764 https://archimer.ifremer.fr/doc/00065/17641/ |
op_rights |
2011 Wiley Periodicals, Inc. info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.1002/tox.20764 |
container_title |
Environmental Toxicology |
container_volume |
28 |
container_issue |
12 |
container_start_page |
689 |
op_container_end_page |
699 |
_version_ |
1766394178032369664 |