Ovarian and sperm regulatory peptides regulate ovulation in the oyster Crassostrea gigas
For more than six decades, several studies have shown that genital products to entering the mantle cavity via the incurrent siphon, initiate in oyster, strong and rhythmic contractions of the adductor muscle (AM). In order to characterize the regulatory peptides capable of triggering AM contractions...
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ftarchimer:oai:archimer.ifremer.fr:1697 2023-05-15T15:58:54+02:00 Ovarian and sperm regulatory peptides regulate ovulation in the oyster Crassostrea gigas Bernay, Benoît Baudy-floc'H, Michele Zanuttini, Bruno Zatylny, Céline Pouvreau, Stephane Henry, Joël 2006-05 application/pdf https://archimer.ifremer.fr/doc/2006/publication-1697.pdf https://doi.org/10.1002/mrd.20472 https://archimer.ifremer.fr/doc/00000/1697/ eng eng Wiley InterScience https://archimer.ifremer.fr/doc/2006/publication-1697.pdf doi:10.1002/mrd.20472 https://archimer.ifremer.fr/doc/00000/1697/ 2006 Wiley InterScience info:eu-repo/semantics/openAccess restricted use Molecular Reproduction and Development (1040-452X) (Wiley InterScience), 2006-05 , Vol. 73 , N. 5 , P. 607-616 Mass spectrometry Myotropin APGWamide Egg laying Molluscan text Publication info:eu-repo/semantics/article 2006 ftarchimer https://doi.org/10.1002/mrd.20472 2021-09-23T20:14:09Z For more than six decades, several studies have shown that genital products to entering the mantle cavity via the incurrent siphon, initiate in oyster, strong and rhythmic contractions of the adductor muscle (AM). In order to characterize the regulatory peptides capable of triggering AM contractions, we focused on the identification of putative myotropic peptides from genital products. Two experimental approaches were developed. The first one, based on a mass spectrometry screening of the male genital products, led to the identification of the tetrapeptide APGWamide. This neuropeptide was also detected in the seminal secretions of the cephalopod Sepia officinalis. In this species, APGWamide is directly involved in the oocyte transport. In Crassostrea, in vitro bioassay demonstrated that APGWamide modulates the AM contractions that insure the release of oocytes in the external medium. Exposure of oysters to a physiological concentration of APGWamide triggered repetitive shell closures. The second experimental approach was based on the monitoring of HPLC purification by a myotropic bioassay using the cuttlefish oviduct contractions as a target. The successive purification steps of the acidic extraction of ovaries from mature female oysters, led to the characterization of the hexapeptide PIESVD. When applied to mature female oysters, this peptide triggered the increase of shell closure frequency. The activity of these two regulatory peptides is the first experimental evidence of a peptidergic control of egg-laying in oyster. APGWamide and PIESVD could be used, in commercial and experimental hatcheries, for the identification of mature females to be selected for in vitro fertilization. Article in Journal/Newspaper Crassostrea gigas Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Molecular Reproduction and Development 73 5 607 616 |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
Mass spectrometry Myotropin APGWamide Egg laying Molluscan |
spellingShingle |
Mass spectrometry Myotropin APGWamide Egg laying Molluscan Bernay, Benoît Baudy-floc'H, Michele Zanuttini, Bruno Zatylny, Céline Pouvreau, Stephane Henry, Joël Ovarian and sperm regulatory peptides regulate ovulation in the oyster Crassostrea gigas |
topic_facet |
Mass spectrometry Myotropin APGWamide Egg laying Molluscan |
description |
For more than six decades, several studies have shown that genital products to entering the mantle cavity via the incurrent siphon, initiate in oyster, strong and rhythmic contractions of the adductor muscle (AM). In order to characterize the regulatory peptides capable of triggering AM contractions, we focused on the identification of putative myotropic peptides from genital products. Two experimental approaches were developed. The first one, based on a mass spectrometry screening of the male genital products, led to the identification of the tetrapeptide APGWamide. This neuropeptide was also detected in the seminal secretions of the cephalopod Sepia officinalis. In this species, APGWamide is directly involved in the oocyte transport. In Crassostrea, in vitro bioassay demonstrated that APGWamide modulates the AM contractions that insure the release of oocytes in the external medium. Exposure of oysters to a physiological concentration of APGWamide triggered repetitive shell closures. The second experimental approach was based on the monitoring of HPLC purification by a myotropic bioassay using the cuttlefish oviduct contractions as a target. The successive purification steps of the acidic extraction of ovaries from mature female oysters, led to the characterization of the hexapeptide PIESVD. When applied to mature female oysters, this peptide triggered the increase of shell closure frequency. The activity of these two regulatory peptides is the first experimental evidence of a peptidergic control of egg-laying in oyster. APGWamide and PIESVD could be used, in commercial and experimental hatcheries, for the identification of mature females to be selected for in vitro fertilization. |
format |
Article in Journal/Newspaper |
author |
Bernay, Benoît Baudy-floc'H, Michele Zanuttini, Bruno Zatylny, Céline Pouvreau, Stephane Henry, Joël |
author_facet |
Bernay, Benoît Baudy-floc'H, Michele Zanuttini, Bruno Zatylny, Céline Pouvreau, Stephane Henry, Joël |
author_sort |
Bernay, Benoît |
title |
Ovarian and sperm regulatory peptides regulate ovulation in the oyster Crassostrea gigas |
title_short |
Ovarian and sperm regulatory peptides regulate ovulation in the oyster Crassostrea gigas |
title_full |
Ovarian and sperm regulatory peptides regulate ovulation in the oyster Crassostrea gigas |
title_fullStr |
Ovarian and sperm regulatory peptides regulate ovulation in the oyster Crassostrea gigas |
title_full_unstemmed |
Ovarian and sperm regulatory peptides regulate ovulation in the oyster Crassostrea gigas |
title_sort |
ovarian and sperm regulatory peptides regulate ovulation in the oyster crassostrea gigas |
publisher |
Wiley InterScience |
publishDate |
2006 |
url |
https://archimer.ifremer.fr/doc/2006/publication-1697.pdf https://doi.org/10.1002/mrd.20472 https://archimer.ifremer.fr/doc/00000/1697/ |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Molecular Reproduction and Development (1040-452X) (Wiley InterScience), 2006-05 , Vol. 73 , N. 5 , P. 607-616 |
op_relation |
https://archimer.ifremer.fr/doc/2006/publication-1697.pdf doi:10.1002/mrd.20472 https://archimer.ifremer.fr/doc/00000/1697/ |
op_rights |
2006 Wiley InterScience info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.1002/mrd.20472 |
container_title |
Molecular Reproduction and Development |
container_volume |
73 |
container_issue |
5 |
container_start_page |
607 |
op_container_end_page |
616 |
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1766394679835754496 |