Effects of cadmium on aneuploidy and hemocyte parameters in the Pacific oyster, Crassostrea gigas
Pacific oysters, Crassostrea gigas, are commonly reared in estuaries where they are exposed to anthropogenic pollution. Much research has been made on the toxicity of cadmium to aquatic organisms because the compound recurrently contaminates their environment. Our study examined the influence of cad...
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ftarchimer:oai:archimer.ifremer.fr:1687 2023-05-15T15:57:47+02:00 Effects of cadmium on aneuploidy and hemocyte parameters in the Pacific oyster, Crassostrea gigas Bouilly, Karine Gagnaire, Beatrice Bonnard, Marc Thomas-guyon, Helene Renault, Tristan Miramand, Pierre Lapegue, Sylvie 2006-06 application/pdf https://archimer.ifremer.fr/doc/2006/publication-1687.pdf https://doi.org/10.1016/j.aquatox.2006.02.028 https://archimer.ifremer.fr/doc/00000/1687/ eng eng Elsevier https://archimer.ifremer.fr/doc/2006/publication-1687.pdf doi:10.1016/j.aquatox.2006.02.028 https://archimer.ifremer.fr/doc/00000/1687/ 2006 Elsevier B.V. All rights reserved info:eu-repo/semantics/openAccess restricted use Aquatic Toxicology (0166-445X) (Elsevier), 2006-06 , Vol. 78 , N. 2 , P. 149-156 Phenoloxidase Flow cytometry Hemocytes Aneuploidy Crassostrea gigas Cadmium text Publication info:eu-repo/semantics/article 2006 ftarchimer https://doi.org/10.1016/j.aquatox.2006.02.028 2021-09-23T20:14:09Z Pacific oysters, Crassostrea gigas, are commonly reared in estuaries where they are exposed to anthropogenic pollution. Much research has been made on the toxicity of cadmium to aquatic organisms because the compound recurrently contaminates their environment. Our study examined the influence of cadmium on aneuploidy level (lowered chromosome number in a percentage of somatic cells) and hemocyte parameters in C. gigas at different stages of life. Adults and juveniles were exposed to two different concentrations of cadmium. The first concentration applied was equivalent to a peak value found in Marennes-Oleron bay (Charente-Maritime, France; 50 ng L-1) and the second was 10 times higher (500 ng L-1). Exposure to 50 ng L-1 cadmium caused a significant decrease in the survival time of C gigas, but exposure to 500 ng L-1 surprisingly affected the survival time positively. Significant differences in aneuploidy level were observed between the cadmium treatments and the control in adults but not in juveniles or the offspring of the adult groups. The effects of cadmium on hemocyte parameters were analyzed by flow cytometry. Several hemocyte parameters increased significantly after 21 days of cadmium exposure and subsequently decreased. Phenoloxidase-like activity, evaluated by spectrophotometry, varied over the time of the experiment and increased after 66 days of contact with 500 ng L-1 cadmium. Taken together, cadmium at environmentally relevant concentrations seems to have only moderate effects on aneuploidy and hemocyte parameters. (c) 2006 Elsevier B.V. All rights reserved. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Pacific Aquatic Toxicology 78 2 149 156 |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
Phenoloxidase Flow cytometry Hemocytes Aneuploidy Crassostrea gigas Cadmium |
spellingShingle |
Phenoloxidase Flow cytometry Hemocytes Aneuploidy Crassostrea gigas Cadmium Bouilly, Karine Gagnaire, Beatrice Bonnard, Marc Thomas-guyon, Helene Renault, Tristan Miramand, Pierre Lapegue, Sylvie Effects of cadmium on aneuploidy and hemocyte parameters in the Pacific oyster, Crassostrea gigas |
topic_facet |
Phenoloxidase Flow cytometry Hemocytes Aneuploidy Crassostrea gigas Cadmium |
description |
Pacific oysters, Crassostrea gigas, are commonly reared in estuaries where they are exposed to anthropogenic pollution. Much research has been made on the toxicity of cadmium to aquatic organisms because the compound recurrently contaminates their environment. Our study examined the influence of cadmium on aneuploidy level (lowered chromosome number in a percentage of somatic cells) and hemocyte parameters in C. gigas at different stages of life. Adults and juveniles were exposed to two different concentrations of cadmium. The first concentration applied was equivalent to a peak value found in Marennes-Oleron bay (Charente-Maritime, France; 50 ng L-1) and the second was 10 times higher (500 ng L-1). Exposure to 50 ng L-1 cadmium caused a significant decrease in the survival time of C gigas, but exposure to 500 ng L-1 surprisingly affected the survival time positively. Significant differences in aneuploidy level were observed between the cadmium treatments and the control in adults but not in juveniles or the offspring of the adult groups. The effects of cadmium on hemocyte parameters were analyzed by flow cytometry. Several hemocyte parameters increased significantly after 21 days of cadmium exposure and subsequently decreased. Phenoloxidase-like activity, evaluated by spectrophotometry, varied over the time of the experiment and increased after 66 days of contact with 500 ng L-1 cadmium. Taken together, cadmium at environmentally relevant concentrations seems to have only moderate effects on aneuploidy and hemocyte parameters. (c) 2006 Elsevier B.V. All rights reserved. |
format |
Article in Journal/Newspaper |
author |
Bouilly, Karine Gagnaire, Beatrice Bonnard, Marc Thomas-guyon, Helene Renault, Tristan Miramand, Pierre Lapegue, Sylvie |
author_facet |
Bouilly, Karine Gagnaire, Beatrice Bonnard, Marc Thomas-guyon, Helene Renault, Tristan Miramand, Pierre Lapegue, Sylvie |
author_sort |
Bouilly, Karine |
title |
Effects of cadmium on aneuploidy and hemocyte parameters in the Pacific oyster, Crassostrea gigas |
title_short |
Effects of cadmium on aneuploidy and hemocyte parameters in the Pacific oyster, Crassostrea gigas |
title_full |
Effects of cadmium on aneuploidy and hemocyte parameters in the Pacific oyster, Crassostrea gigas |
title_fullStr |
Effects of cadmium on aneuploidy and hemocyte parameters in the Pacific oyster, Crassostrea gigas |
title_full_unstemmed |
Effects of cadmium on aneuploidy and hemocyte parameters in the Pacific oyster, Crassostrea gigas |
title_sort |
effects of cadmium on aneuploidy and hemocyte parameters in the pacific oyster, crassostrea gigas |
publisher |
Elsevier |
publishDate |
2006 |
url |
https://archimer.ifremer.fr/doc/2006/publication-1687.pdf https://doi.org/10.1016/j.aquatox.2006.02.028 https://archimer.ifremer.fr/doc/00000/1687/ |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Aquatic Toxicology (0166-445X) (Elsevier), 2006-06 , Vol. 78 , N. 2 , P. 149-156 |
op_relation |
https://archimer.ifremer.fr/doc/2006/publication-1687.pdf doi:10.1016/j.aquatox.2006.02.028 https://archimer.ifremer.fr/doc/00000/1687/ |
op_rights |
2006 Elsevier B.V. All rights reserved info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.1016/j.aquatox.2006.02.028 |
container_title |
Aquatic Toxicology |
container_volume |
78 |
container_issue |
2 |
container_start_page |
149 |
op_container_end_page |
156 |
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1766393475268345856 |