Suppression substractive hybridisation (SSH) and real time PCR reveal differential gene expression in the Pacific cupped oyster, Crassostrea gigas, challenged with Ostreid herpesvirus 1
Virus-induced genes were identified using suppression subtractive hybridisation (SSH) from Pacific cupped oyster, Crassostrea gigas, haemocytes challenged by OsHV-1. A total of 304 clones from SSH forward library were sequenced. Among these sequences, some homologues corresponded to (i) immune relat...
Published in: | Developmental & Comparative Immunology |
---|---|
Main Authors: | , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Elsevier
2011
|
Subjects: | |
Online Access: | https://archimer.ifremer.fr/doc/00043/15466/12843.pdf https://doi.org/10.1016/j.dci.2011.02.004 https://archimer.ifremer.fr/doc/00043/15466/ |
id |
ftarchimer:oai:archimer.ifremer.fr:15466 |
---|---|
record_format |
openpolar |
spelling |
ftarchimer:oai:archimer.ifremer.fr:15466 2023-05-15T15:57:39+02:00 Suppression substractive hybridisation (SSH) and real time PCR reveal differential gene expression in the Pacific cupped oyster, Crassostrea gigas, challenged with Ostreid herpesvirus 1 Renault, Tristan Faury, Nicole Barbosa Solomieu, Valérie Moreau, Kevin 2011-07 application/pdf https://archimer.ifremer.fr/doc/00043/15466/12843.pdf https://doi.org/10.1016/j.dci.2011.02.004 https://archimer.ifremer.fr/doc/00043/15466/ eng eng Elsevier https://archimer.ifremer.fr/doc/00043/15466/12843.pdf doi:10.1016/j.dci.2011.02.004 https://archimer.ifremer.fr/doc/00043/15466/ 2011 Elsevier Ltd. All rights reserved. info:eu-repo/semantics/openAccess restricted use Developmental & Comparative Immunology (0145-305X) (Elsevier), 2011-07 , Vol. 35 , N. 7 , P. 725-735 Pacific cupped oyster Crassostrea gigas OsHV-1 Immunity Gene expression Multicopper oxidase Macrophage expressed protein Ig cell adhesion molecule Interferon-induced protein 44 Virus RNA text Publication info:eu-repo/semantics/article 2011 ftarchimer https://doi.org/10.1016/j.dci.2011.02.004 2021-09-23T20:19:41Z Virus-induced genes were identified using suppression subtractive hybridisation (SSH) from Pacific cupped oyster, Crassostrea gigas, haemocytes challenged by OsHV-1. A total of 304 clones from SSH forward library were sequenced. Among these sequences, some homologues corresponded to (i) immune related genes (macrophage express protein, IK cytokine, interferon-induced protein 44 or multicopper oxidase), (ii) apoptosis related genes (Bcl-2) and (iii) cell signalling and virus receptor genes (glypican). Molecular characterization and phylogenic analysis of 3 immune-related genes (macrophage expressed protein, multicopper oxidase and immunoglobulin domain cell adhesion molecule) were performed. Finally, quantitative PCR revealed significant changes in the expression of immune related genes (multicopper oxidase, macrophage expressed protein, myeloid differentiation factor 88 and interferon-induced protein 44) in oysters experimentally challenged with OsHV-1. These findings provide a first basis for studying the role of innate immunity in response to viruses in bivalves and identified genes may serve as markers of interest in breeding programs in order to obtain selected oysters presenting OsHV-1 resistance. Article in Journal/Newspaper Crassostrea gigas Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Pacific Developmental & Comparative Immunology 35 7 725 735 |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
Pacific cupped oyster Crassostrea gigas OsHV-1 Immunity Gene expression Multicopper oxidase Macrophage expressed protein Ig cell adhesion molecule Interferon-induced protein 44 Virus RNA |
spellingShingle |
Pacific cupped oyster Crassostrea gigas OsHV-1 Immunity Gene expression Multicopper oxidase Macrophage expressed protein Ig cell adhesion molecule Interferon-induced protein 44 Virus RNA Renault, Tristan Faury, Nicole Barbosa Solomieu, Valérie Moreau, Kevin Suppression substractive hybridisation (SSH) and real time PCR reveal differential gene expression in the Pacific cupped oyster, Crassostrea gigas, challenged with Ostreid herpesvirus 1 |
topic_facet |
Pacific cupped oyster Crassostrea gigas OsHV-1 Immunity Gene expression Multicopper oxidase Macrophage expressed protein Ig cell adhesion molecule Interferon-induced protein 44 Virus RNA |
description |
Virus-induced genes were identified using suppression subtractive hybridisation (SSH) from Pacific cupped oyster, Crassostrea gigas, haemocytes challenged by OsHV-1. A total of 304 clones from SSH forward library were sequenced. Among these sequences, some homologues corresponded to (i) immune related genes (macrophage express protein, IK cytokine, interferon-induced protein 44 or multicopper oxidase), (ii) apoptosis related genes (Bcl-2) and (iii) cell signalling and virus receptor genes (glypican). Molecular characterization and phylogenic analysis of 3 immune-related genes (macrophage expressed protein, multicopper oxidase and immunoglobulin domain cell adhesion molecule) were performed. Finally, quantitative PCR revealed significant changes in the expression of immune related genes (multicopper oxidase, macrophage expressed protein, myeloid differentiation factor 88 and interferon-induced protein 44) in oysters experimentally challenged with OsHV-1. These findings provide a first basis for studying the role of innate immunity in response to viruses in bivalves and identified genes may serve as markers of interest in breeding programs in order to obtain selected oysters presenting OsHV-1 resistance. |
format |
Article in Journal/Newspaper |
author |
Renault, Tristan Faury, Nicole Barbosa Solomieu, Valérie Moreau, Kevin |
author_facet |
Renault, Tristan Faury, Nicole Barbosa Solomieu, Valérie Moreau, Kevin |
author_sort |
Renault, Tristan |
title |
Suppression substractive hybridisation (SSH) and real time PCR reveal differential gene expression in the Pacific cupped oyster, Crassostrea gigas, challenged with Ostreid herpesvirus 1 |
title_short |
Suppression substractive hybridisation (SSH) and real time PCR reveal differential gene expression in the Pacific cupped oyster, Crassostrea gigas, challenged with Ostreid herpesvirus 1 |
title_full |
Suppression substractive hybridisation (SSH) and real time PCR reveal differential gene expression in the Pacific cupped oyster, Crassostrea gigas, challenged with Ostreid herpesvirus 1 |
title_fullStr |
Suppression substractive hybridisation (SSH) and real time PCR reveal differential gene expression in the Pacific cupped oyster, Crassostrea gigas, challenged with Ostreid herpesvirus 1 |
title_full_unstemmed |
Suppression substractive hybridisation (SSH) and real time PCR reveal differential gene expression in the Pacific cupped oyster, Crassostrea gigas, challenged with Ostreid herpesvirus 1 |
title_sort |
suppression substractive hybridisation (ssh) and real time pcr reveal differential gene expression in the pacific cupped oyster, crassostrea gigas, challenged with ostreid herpesvirus 1 |
publisher |
Elsevier |
publishDate |
2011 |
url |
https://archimer.ifremer.fr/doc/00043/15466/12843.pdf https://doi.org/10.1016/j.dci.2011.02.004 https://archimer.ifremer.fr/doc/00043/15466/ |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Developmental & Comparative Immunology (0145-305X) (Elsevier), 2011-07 , Vol. 35 , N. 7 , P. 725-735 |
op_relation |
https://archimer.ifremer.fr/doc/00043/15466/12843.pdf doi:10.1016/j.dci.2011.02.004 https://archimer.ifremer.fr/doc/00043/15466/ |
op_rights |
2011 Elsevier Ltd. All rights reserved. info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.1016/j.dci.2011.02.004 |
container_title |
Developmental & Comparative Immunology |
container_volume |
35 |
container_issue |
7 |
container_start_page |
725 |
op_container_end_page |
735 |
_version_ |
1766393328859873280 |