Strain-Dependent Norovirus Bioaccumulation in Oysters
Noroviruses (NoVs) are the main agents of gastroenteritis in humans and the primary pathogens of shellfish-related outbreaks. Some NoV strains bind to shellfish tissues by using carbohydrate structures similar to their human ligands, leading to the hypothesis that such ligands may influence bioaccum...
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ftarchimer:oai:archimer.ifremer.fr:14753 2023-05-15T15:58:46+02:00 Strain-Dependent Norovirus Bioaccumulation in Oysters Maalouf, Haifa Schaeffer, Julien Parnaudeau, Sylvain Le Pendu, Jacques Atmar, Robert L. Crawford, Sue E. Le Guyader, Soizick 2011-05 application/pdf https://archimer.ifremer.fr/doc/00036/14753/12087.pdf https://doi.org/10.1128/AEM.03010-10 https://archimer.ifremer.fr/doc/00036/14753/ eng eng Amer Soc Microbiology https://archimer.ifremer.fr/doc/00036/14753/12087.pdf doi:10.1128/AEM.03010-10 https://archimer.ifremer.fr/doc/00036/14753/ 2011, American Society for Microbiology. All Rights Reserved. info:eu-repo/semantics/openAccess restricted use Applied And Environmental Microbiology (0099-2240) (Amer Soc Microbiology), 2011-05 , Vol. 77 , N. 10 , P. 3189-3196 text Publication info:eu-repo/semantics/article 2011 ftarchimer https://doi.org/10.1128/AEM.03010-10 2021-09-23T20:19:29Z Noroviruses (NoVs) are the main agents of gastroenteritis in humans and the primary pathogens of shellfish-related outbreaks. Some NoV strains bind to shellfish tissues by using carbohydrate structures similar to their human ligands, leading to the hypothesis that such ligands may influence bioaccumulation. This study compares the bioaccumulation efficiencies and tissue distributions in oysters (Crassostrea gigas) of three strains from the two principal human norovirus genogroups. Clear differences between strains were observed. The GI.1 strain was the most efficiently concentrated strain. Bioaccumulation specifically occurred in digestive tissues in a dose-dependent manner, and its efficiency paralleled ligand expression, which was highest during the cold months. In comparison, the GII.4 strain was very poorly bioaccumulated and was recovered in almost all tissues without seasonal influence. The GII.3 strain presented an intermediate behavior, without seasonal effect and with less bioaccumulation efficiency than that of the GI.1 strain during the cold months. In addition, the GII.3 strain was transiently concentrated in gills and mantle before being almost specifically accumulated in digestive tissues. Carbohydrate ligand specificities of the strains at least partly explain the strain-dependent bioaccumulation characteristics. In particular, binding to the digestive-tube-specific ligand should contribute to bioaccumulation, whereas we hypothesize that binding to the sialic acid-containing ligand present in all tissues would contribute to retain virus particles in the gills or mantle and lead to rapid destruction. Article in Journal/Newspaper Crassostrea gigas Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Applied and Environmental Microbiology 77 10 3189 3196 |
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Open Polar |
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Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
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ftarchimer |
language |
English |
description |
Noroviruses (NoVs) are the main agents of gastroenteritis in humans and the primary pathogens of shellfish-related outbreaks. Some NoV strains bind to shellfish tissues by using carbohydrate structures similar to their human ligands, leading to the hypothesis that such ligands may influence bioaccumulation. This study compares the bioaccumulation efficiencies and tissue distributions in oysters (Crassostrea gigas) of three strains from the two principal human norovirus genogroups. Clear differences between strains were observed. The GI.1 strain was the most efficiently concentrated strain. Bioaccumulation specifically occurred in digestive tissues in a dose-dependent manner, and its efficiency paralleled ligand expression, which was highest during the cold months. In comparison, the GII.4 strain was very poorly bioaccumulated and was recovered in almost all tissues without seasonal influence. The GII.3 strain presented an intermediate behavior, without seasonal effect and with less bioaccumulation efficiency than that of the GI.1 strain during the cold months. In addition, the GII.3 strain was transiently concentrated in gills and mantle before being almost specifically accumulated in digestive tissues. Carbohydrate ligand specificities of the strains at least partly explain the strain-dependent bioaccumulation characteristics. In particular, binding to the digestive-tube-specific ligand should contribute to bioaccumulation, whereas we hypothesize that binding to the sialic acid-containing ligand present in all tissues would contribute to retain virus particles in the gills or mantle and lead to rapid destruction. |
format |
Article in Journal/Newspaper |
author |
Maalouf, Haifa Schaeffer, Julien Parnaudeau, Sylvain Le Pendu, Jacques Atmar, Robert L. Crawford, Sue E. Le Guyader, Soizick |
spellingShingle |
Maalouf, Haifa Schaeffer, Julien Parnaudeau, Sylvain Le Pendu, Jacques Atmar, Robert L. Crawford, Sue E. Le Guyader, Soizick Strain-Dependent Norovirus Bioaccumulation in Oysters |
author_facet |
Maalouf, Haifa Schaeffer, Julien Parnaudeau, Sylvain Le Pendu, Jacques Atmar, Robert L. Crawford, Sue E. Le Guyader, Soizick |
author_sort |
Maalouf, Haifa |
title |
Strain-Dependent Norovirus Bioaccumulation in Oysters |
title_short |
Strain-Dependent Norovirus Bioaccumulation in Oysters |
title_full |
Strain-Dependent Norovirus Bioaccumulation in Oysters |
title_fullStr |
Strain-Dependent Norovirus Bioaccumulation in Oysters |
title_full_unstemmed |
Strain-Dependent Norovirus Bioaccumulation in Oysters |
title_sort |
strain-dependent norovirus bioaccumulation in oysters |
publisher |
Amer Soc Microbiology |
publishDate |
2011 |
url |
https://archimer.ifremer.fr/doc/00036/14753/12087.pdf https://doi.org/10.1128/AEM.03010-10 https://archimer.ifremer.fr/doc/00036/14753/ |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Applied And Environmental Microbiology (0099-2240) (Amer Soc Microbiology), 2011-05 , Vol. 77 , N. 10 , P. 3189-3196 |
op_relation |
https://archimer.ifremer.fr/doc/00036/14753/12087.pdf doi:10.1128/AEM.03010-10 https://archimer.ifremer.fr/doc/00036/14753/ |
op_rights |
2011, American Society for Microbiology. All Rights Reserved. info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.1128/AEM.03010-10 |
container_title |
Applied and Environmental Microbiology |
container_volume |
77 |
container_issue |
10 |
container_start_page |
3189 |
op_container_end_page |
3196 |
_version_ |
1766394544160505856 |