Transcriptomic response of the Pacific oyster Crassostrea gigas to hypoxia
Marine intertidal organisms commonly face hypoxic stress during low tide emersion; moreover, eutrophic conditions and sediment nearness could lead to hypoxic phenomena; it is indeed important to understand the molecular processes involved in the response to hypoxia. In this study the molecular respo...
Published in: | Marine Genomics |
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Language: | English |
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Elsevier Science Bv
2010
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Online Access: | https://archimer.ifremer.fr/doc/00027/13799/11053.pdf https://archimer.ifremer.fr/doc/00027/13799/11055.pdf https://archimer.ifremer.fr/doc/00027/13799/11056.pdf https://archimer.ifremer.fr/doc/00027/13799/11057.pdf https://doi.org/10.1016/j.margen.2010.08.005 https://archimer.ifremer.fr/doc/00027/13799/ |
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ftarchimer:oai:archimer.ifremer.fr:13799 2023-05-15T15:57:58+02:00 Transcriptomic response of the Pacific oyster Crassostrea gigas to hypoxia Sussarellu, Rossana Fabioux, Caroline Le Moullac, Gilles Fleury, Elodie Moraga, Dario 2010-09 application/pdf https://archimer.ifremer.fr/doc/00027/13799/11053.pdf https://archimer.ifremer.fr/doc/00027/13799/11055.pdf https://archimer.ifremer.fr/doc/00027/13799/11056.pdf https://archimer.ifremer.fr/doc/00027/13799/11057.pdf https://doi.org/10.1016/j.margen.2010.08.005 https://archimer.ifremer.fr/doc/00027/13799/ eng eng Elsevier Science Bv https://archimer.ifremer.fr/doc/00027/13799/11053.pdf https://archimer.ifremer.fr/doc/00027/13799/11055.pdf https://archimer.ifremer.fr/doc/00027/13799/11056.pdf https://archimer.ifremer.fr/doc/00027/13799/11057.pdf doi:10.1016/j.margen.2010.08.005 https://archimer.ifremer.fr/doc/00027/13799/ 2010 Elsevier B.V. All rights reserved. info:eu-repo/semantics/openAccess restricted use Marine Genomics (1874-7787) (Elsevier Science Bv), 2010-09 , Vol. 3 , N. 3-4 , P. 133-143 Mollusca Hypoxia cDNA microarray Gene expression Oxidative stress Respiratory chain text Publication info:eu-repo/semantics/article 2010 ftarchimer https://doi.org/10.1016/j.margen.2010.08.005 2021-09-23T20:19:06Z Marine intertidal organisms commonly face hypoxic stress during low tide emersion; moreover, eutrophic conditions and sediment nearness could lead to hypoxic phenomena; it is indeed important to understand the molecular processes involved in the response to hypoxia. In this study the molecular response of the Pacific oyster Crassostrea gigas to prolonged hypoxia (2 mg O-2 L-1 for 20 d) was investigated under experimental conditions. A transcriptomic approach was employed using a cDNA microarray of 9058 C. gigas clones to highlight the genetic expression patterns of the Pacific oyster under hypoxic conditions. Lines of oysters resistant (R) and susceptible (S) to summer mortality were used in this study. ANOVA analysis was used to identify the genes involved in the response to hypoxia in comparison to normoxic conditions. The hypoxic response was maximal at day 20. The principal biological processes up-regulated by hypoxic stress were antioxidant defense and the respiratory chain compartment, suggesting oxidative stress caused by hypoxia or an anticipatory response for normoxic recovery. This is the first study employing microarrays to characterize the genetic markers and metabolic pathways responding to hypoxic stress in C gigas. (C) 2010 Elsevier B.V. All rights reserved. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Pacific Marine Genomics 3 3-4 133 143 |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
Mollusca Hypoxia cDNA microarray Gene expression Oxidative stress Respiratory chain |
spellingShingle |
Mollusca Hypoxia cDNA microarray Gene expression Oxidative stress Respiratory chain Sussarellu, Rossana Fabioux, Caroline Le Moullac, Gilles Fleury, Elodie Moraga, Dario Transcriptomic response of the Pacific oyster Crassostrea gigas to hypoxia |
topic_facet |
Mollusca Hypoxia cDNA microarray Gene expression Oxidative stress Respiratory chain |
description |
Marine intertidal organisms commonly face hypoxic stress during low tide emersion; moreover, eutrophic conditions and sediment nearness could lead to hypoxic phenomena; it is indeed important to understand the molecular processes involved in the response to hypoxia. In this study the molecular response of the Pacific oyster Crassostrea gigas to prolonged hypoxia (2 mg O-2 L-1 for 20 d) was investigated under experimental conditions. A transcriptomic approach was employed using a cDNA microarray of 9058 C. gigas clones to highlight the genetic expression patterns of the Pacific oyster under hypoxic conditions. Lines of oysters resistant (R) and susceptible (S) to summer mortality were used in this study. ANOVA analysis was used to identify the genes involved in the response to hypoxia in comparison to normoxic conditions. The hypoxic response was maximal at day 20. The principal biological processes up-regulated by hypoxic stress were antioxidant defense and the respiratory chain compartment, suggesting oxidative stress caused by hypoxia or an anticipatory response for normoxic recovery. This is the first study employing microarrays to characterize the genetic markers and metabolic pathways responding to hypoxic stress in C gigas. (C) 2010 Elsevier B.V. All rights reserved. |
format |
Article in Journal/Newspaper |
author |
Sussarellu, Rossana Fabioux, Caroline Le Moullac, Gilles Fleury, Elodie Moraga, Dario |
author_facet |
Sussarellu, Rossana Fabioux, Caroline Le Moullac, Gilles Fleury, Elodie Moraga, Dario |
author_sort |
Sussarellu, Rossana |
title |
Transcriptomic response of the Pacific oyster Crassostrea gigas to hypoxia |
title_short |
Transcriptomic response of the Pacific oyster Crassostrea gigas to hypoxia |
title_full |
Transcriptomic response of the Pacific oyster Crassostrea gigas to hypoxia |
title_fullStr |
Transcriptomic response of the Pacific oyster Crassostrea gigas to hypoxia |
title_full_unstemmed |
Transcriptomic response of the Pacific oyster Crassostrea gigas to hypoxia |
title_sort |
transcriptomic response of the pacific oyster crassostrea gigas to hypoxia |
publisher |
Elsevier Science Bv |
publishDate |
2010 |
url |
https://archimer.ifremer.fr/doc/00027/13799/11053.pdf https://archimer.ifremer.fr/doc/00027/13799/11055.pdf https://archimer.ifremer.fr/doc/00027/13799/11056.pdf https://archimer.ifremer.fr/doc/00027/13799/11057.pdf https://doi.org/10.1016/j.margen.2010.08.005 https://archimer.ifremer.fr/doc/00027/13799/ |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Marine Genomics (1874-7787) (Elsevier Science Bv), 2010-09 , Vol. 3 , N. 3-4 , P. 133-143 |
op_relation |
https://archimer.ifremer.fr/doc/00027/13799/11053.pdf https://archimer.ifremer.fr/doc/00027/13799/11055.pdf https://archimer.ifremer.fr/doc/00027/13799/11056.pdf https://archimer.ifremer.fr/doc/00027/13799/11057.pdf doi:10.1016/j.margen.2010.08.005 https://archimer.ifremer.fr/doc/00027/13799/ |
op_rights |
2010 Elsevier B.V. All rights reserved. info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.1016/j.margen.2010.08.005 |
container_title |
Marine Genomics |
container_volume |
3 |
container_issue |
3-4 |
container_start_page |
133 |
op_container_end_page |
143 |
_version_ |
1766393684012564480 |