Vibriosis induced by experimental cohabitation in Crassostrea gigas: Evidence of early infection and down-expression of immune-related genes
The understanding of reciprocal interactions between Crassostrea gigas and Vibrio sp., whether these be virulent or avirulent, is vital for the development of methods to improve the health status of cultured oysters. We describe an original non-invasive experimental infection technique using cohabit...
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Academic Press Ltd- Elsevier Science Ltd
2011
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Online Access: | https://archimer.ifremer.fr/doc/00025/13604/10681.pdf https://doi.org/10.1016/j.fsi.2010.12.017 https://archimer.ifremer.fr/doc/00025/13604/ |
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ftarchimer:oai:archimer.ifremer.fr:13604 2023-05-15T15:58:26+02:00 Vibriosis induced by experimental cohabitation in Crassostrea gigas: Evidence of early infection and down-expression of immune-related genes De Decker, Sophie Saulnier, Denis 2011-02 application/pdf https://archimer.ifremer.fr/doc/00025/13604/10681.pdf https://doi.org/10.1016/j.fsi.2010.12.017 https://archimer.ifremer.fr/doc/00025/13604/ eng eng Academic Press Ltd- Elsevier Science Ltd https://archimer.ifremer.fr/doc/00025/13604/10681.pdf doi:10.1016/j.fsi.2010.12.017 https://archimer.ifremer.fr/doc/00025/13604/ 2011 Elsevier Ltd All rights reserved. info:eu-repo/semantics/openAccess restricted use Fish & Shellfish Immunology (1050-4648) (Academic Press Ltd- Elsevier Science Ltd), 2011-02 , Vol. 30 , N. 2 , P. 691-699 Oyster-Vibrio interactions Pathogenesis Immune response Non-invasive experimental challenge Real-time PCR text Publication info:eu-repo/semantics/article 2011 ftarchimer https://doi.org/10.1016/j.fsi.2010.12.017 2021-09-23T20:19:06Z The understanding of reciprocal interactions between Crassostrea gigas and Vibrio sp., whether these be virulent or avirulent, is vital for the development of methods to improve the health status of cultured oysters. We describe an original non-invasive experimental infection technique using cohabitation, designed to explore these interactions. Using real-time PCR techniques we examined the dynamics of virulent and avirulent Vibrio sp. in oyster hemolymph and tank seawater, and made a parallel study of the expression of four genes involved in oyster immune defense: Cg-BPI, Cg-EcSOD, Cg-IκB, Cg-TIMP. No mortality occurred in control animals, but oysters put in cohabitation for 2–48 h with animals previously infected by two Vibrio pathogens suffered mortalities from 2 to 16 days post-cohabitation. Our results show that virulent Vibrio infect healthy individuals after only 2 h of cohabitation, with values ranging from 4.5×102 to 2×104 cells ml−1 hemolymph. Simultaneously, an approximate ten-fold increase of the total Vibrio population was observed in control animals, with a 6.6–78.5-fold up-expression of targeted genes. In contrast, oysters exposed to harmful bacteria had mean expression levels strongly down-regulated by a factor of 9.2–29 (depending on the gene) compared with control animals. Although oysters were still found to be infected by virulent Vibrio after 6–48 h of cohabitation, no significant differences were noted when comparing levels of each transcript in control and infected oysters at the same sampling times during this period: the important differences were noted before 6 h cohabitation. Taken together, our data support (1) the hypothesis that virulent Vibrio disturbs the immune response of this invertebrate host both rapidly and significantly, although this occurs specifically during an early and transient period during the first 6 h of cohabitation challenge, and that (2) expression of targeted genes is not correlated with vibriosis resistance. Article in Journal/Newspaper Crassostrea gigas Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Fish & Shellfish Immunology 30 2 691 699 |
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Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
Oyster-Vibrio interactions Pathogenesis Immune response Non-invasive experimental challenge Real-time PCR |
spellingShingle |
Oyster-Vibrio interactions Pathogenesis Immune response Non-invasive experimental challenge Real-time PCR De Decker, Sophie Saulnier, Denis Vibriosis induced by experimental cohabitation in Crassostrea gigas: Evidence of early infection and down-expression of immune-related genes |
topic_facet |
Oyster-Vibrio interactions Pathogenesis Immune response Non-invasive experimental challenge Real-time PCR |
description |
The understanding of reciprocal interactions between Crassostrea gigas and Vibrio sp., whether these be virulent or avirulent, is vital for the development of methods to improve the health status of cultured oysters. We describe an original non-invasive experimental infection technique using cohabitation, designed to explore these interactions. Using real-time PCR techniques we examined the dynamics of virulent and avirulent Vibrio sp. in oyster hemolymph and tank seawater, and made a parallel study of the expression of four genes involved in oyster immune defense: Cg-BPI, Cg-EcSOD, Cg-IκB, Cg-TIMP. No mortality occurred in control animals, but oysters put in cohabitation for 2–48 h with animals previously infected by two Vibrio pathogens suffered mortalities from 2 to 16 days post-cohabitation. Our results show that virulent Vibrio infect healthy individuals after only 2 h of cohabitation, with values ranging from 4.5×102 to 2×104 cells ml−1 hemolymph. Simultaneously, an approximate ten-fold increase of the total Vibrio population was observed in control animals, with a 6.6–78.5-fold up-expression of targeted genes. In contrast, oysters exposed to harmful bacteria had mean expression levels strongly down-regulated by a factor of 9.2–29 (depending on the gene) compared with control animals. Although oysters were still found to be infected by virulent Vibrio after 6–48 h of cohabitation, no significant differences were noted when comparing levels of each transcript in control and infected oysters at the same sampling times during this period: the important differences were noted before 6 h cohabitation. Taken together, our data support (1) the hypothesis that virulent Vibrio disturbs the immune response of this invertebrate host both rapidly and significantly, although this occurs specifically during an early and transient period during the first 6 h of cohabitation challenge, and that (2) expression of targeted genes is not correlated with vibriosis resistance. |
format |
Article in Journal/Newspaper |
author |
De Decker, Sophie Saulnier, Denis |
author_facet |
De Decker, Sophie Saulnier, Denis |
author_sort |
De Decker, Sophie |
title |
Vibriosis induced by experimental cohabitation in Crassostrea gigas: Evidence of early infection and down-expression of immune-related genes |
title_short |
Vibriosis induced by experimental cohabitation in Crassostrea gigas: Evidence of early infection and down-expression of immune-related genes |
title_full |
Vibriosis induced by experimental cohabitation in Crassostrea gigas: Evidence of early infection and down-expression of immune-related genes |
title_fullStr |
Vibriosis induced by experimental cohabitation in Crassostrea gigas: Evidence of early infection and down-expression of immune-related genes |
title_full_unstemmed |
Vibriosis induced by experimental cohabitation in Crassostrea gigas: Evidence of early infection and down-expression of immune-related genes |
title_sort |
vibriosis induced by experimental cohabitation in crassostrea gigas: evidence of early infection and down-expression of immune-related genes |
publisher |
Academic Press Ltd- Elsevier Science Ltd |
publishDate |
2011 |
url |
https://archimer.ifremer.fr/doc/00025/13604/10681.pdf https://doi.org/10.1016/j.fsi.2010.12.017 https://archimer.ifremer.fr/doc/00025/13604/ |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Fish & Shellfish Immunology (1050-4648) (Academic Press Ltd- Elsevier Science Ltd), 2011-02 , Vol. 30 , N. 2 , P. 691-699 |
op_relation |
https://archimer.ifremer.fr/doc/00025/13604/10681.pdf doi:10.1016/j.fsi.2010.12.017 https://archimer.ifremer.fr/doc/00025/13604/ |
op_rights |
2011 Elsevier Ltd All rights reserved. info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.1016/j.fsi.2010.12.017 |
container_title |
Fish & Shellfish Immunology |
container_volume |
30 |
container_issue |
2 |
container_start_page |
691 |
op_container_end_page |
699 |
_version_ |
1766394177052999680 |