The major outer membrane protein OmpU of Vibrio splendidus contributes to host antimicrobial peptide resistance and is required for virulence in the oyster Crassostrea gigas
Vibrio splendidus, strain LGP32, is an oyster pathogen associated with the summer mortalities affecting the production of Crassostrea gigas oysters worldwide. Vibrio splendidus LGP32 was shown to resist to up to 10 mu M Cg-Def defensin and Cg-BPI bactericidal permeability increasing protein, two ant...
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2010
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Online Access: | https://archimer.ifremer.fr/doc/00003/11421/8044.pdf https://doi.org/10.1111/j.1462-2920.2009.02138.x https://archimer.ifremer.fr/doc/00003/11421/ |
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ftarchimer:oai:archimer.ifremer.fr:11421 2023-05-15T15:58:15+02:00 The major outer membrane protein OmpU of Vibrio splendidus contributes to host antimicrobial peptide resistance and is required for virulence in the oyster Crassostrea gigas Duperthuy, Marylise Binesse, Johan Le Roux, Frederique Romestand, Bernard Caro, Audrey Got, Patrice Givaudan, Alain Mazel, Didier Bachere, Evelyne Destoumieux Garzon, Delphine 2010-04 application/pdf https://archimer.ifremer.fr/doc/00003/11421/8044.pdf https://doi.org/10.1111/j.1462-2920.2009.02138.x https://archimer.ifremer.fr/doc/00003/11421/ eng eng Wiley-blackwell Publishing, Inc https://archimer.ifremer.fr/doc/00003/11421/8044.pdf doi:10.1111/j.1462-2920.2009.02138.x https://archimer.ifremer.fr/doc/00003/11421/ 2010 Society for Applied Microbiology and Blackwell Publishing Ltd info:eu-repo/semantics/openAccess restricted use Environmental Microbiology (1462-2912) (Wiley-blackwell Publishing, Inc), 2010-04 , Vol. 12 , N. 4 , P. 951-963 text Publication info:eu-repo/semantics/article 2010 ftarchimer https://doi.org/10.1111/j.1462-2920.2009.02138.x 2021-09-23T20:18:15Z Vibrio splendidus, strain LGP32, is an oyster pathogen associated with the summer mortalities affecting the production of Crassostrea gigas oysters worldwide. Vibrio splendidus LGP32 was shown to resist to up to 10 mu M Cg-Def defensin and Cg-BPI bactericidal permeability increasing protein, two antimicrobial peptides/proteins (AMPs) involved in C. gigas immunity. The resistance to both oyster Cg-Def and Cg-BPI and standard AMPs (polymyxin B, protegrin, human BPI) was dependent on the ompU gene. Indeed, upon ompU inactivation, minimal bactericidal concentrations decreased by up to fourfold. AMP resistance was restored upon ectopic expression of ompU. The susceptibility of bacterial membranes to AMP-induced damages was independent of the ompU-mediated AMP resistance. Besides its role in AMP resistance, ompU proved to be essential for the adherence of V. splendidus LGP32 to fibronectin. Interestingly, in vivo, ompU was identified as a major determinant of V. spiendidus pathogenicity in oyster experimental infections. Indeed, the V. splendidus-induced oyster mortalities dropped from 56% to 11% upon ompU mutation (Kaplan Meier survival curves, P < 0.01). Moreover, in co-infection assays, the ompU mutant was out competed by the wild-type strain with competitive indexes in the range of 0.1-0.2. From this study, ompU is required for virulence of V. splendidus. Contributing to AMP resistance, conferring adhesive properties to V. splendidus, and being essential for in vivo fitness, the OmpU porin appears as an essential effector of the C. gigas/V. splendidus interaction. Article in Journal/Newspaper Crassostrea gigas Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Meier ENVELOPE(-45.900,-45.900,-60.633,-60.633) Environmental Microbiology 12 4 951 963 |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
description |
Vibrio splendidus, strain LGP32, is an oyster pathogen associated with the summer mortalities affecting the production of Crassostrea gigas oysters worldwide. Vibrio splendidus LGP32 was shown to resist to up to 10 mu M Cg-Def defensin and Cg-BPI bactericidal permeability increasing protein, two antimicrobial peptides/proteins (AMPs) involved in C. gigas immunity. The resistance to both oyster Cg-Def and Cg-BPI and standard AMPs (polymyxin B, protegrin, human BPI) was dependent on the ompU gene. Indeed, upon ompU inactivation, minimal bactericidal concentrations decreased by up to fourfold. AMP resistance was restored upon ectopic expression of ompU. The susceptibility of bacterial membranes to AMP-induced damages was independent of the ompU-mediated AMP resistance. Besides its role in AMP resistance, ompU proved to be essential for the adherence of V. splendidus LGP32 to fibronectin. Interestingly, in vivo, ompU was identified as a major determinant of V. spiendidus pathogenicity in oyster experimental infections. Indeed, the V. splendidus-induced oyster mortalities dropped from 56% to 11% upon ompU mutation (Kaplan Meier survival curves, P < 0.01). Moreover, in co-infection assays, the ompU mutant was out competed by the wild-type strain with competitive indexes in the range of 0.1-0.2. From this study, ompU is required for virulence of V. splendidus. Contributing to AMP resistance, conferring adhesive properties to V. splendidus, and being essential for in vivo fitness, the OmpU porin appears as an essential effector of the C. gigas/V. splendidus interaction. |
format |
Article in Journal/Newspaper |
author |
Duperthuy, Marylise Binesse, Johan Le Roux, Frederique Romestand, Bernard Caro, Audrey Got, Patrice Givaudan, Alain Mazel, Didier Bachere, Evelyne Destoumieux Garzon, Delphine |
spellingShingle |
Duperthuy, Marylise Binesse, Johan Le Roux, Frederique Romestand, Bernard Caro, Audrey Got, Patrice Givaudan, Alain Mazel, Didier Bachere, Evelyne Destoumieux Garzon, Delphine The major outer membrane protein OmpU of Vibrio splendidus contributes to host antimicrobial peptide resistance and is required for virulence in the oyster Crassostrea gigas |
author_facet |
Duperthuy, Marylise Binesse, Johan Le Roux, Frederique Romestand, Bernard Caro, Audrey Got, Patrice Givaudan, Alain Mazel, Didier Bachere, Evelyne Destoumieux Garzon, Delphine |
author_sort |
Duperthuy, Marylise |
title |
The major outer membrane protein OmpU of Vibrio splendidus contributes to host antimicrobial peptide resistance and is required for virulence in the oyster Crassostrea gigas |
title_short |
The major outer membrane protein OmpU of Vibrio splendidus contributes to host antimicrobial peptide resistance and is required for virulence in the oyster Crassostrea gigas |
title_full |
The major outer membrane protein OmpU of Vibrio splendidus contributes to host antimicrobial peptide resistance and is required for virulence in the oyster Crassostrea gigas |
title_fullStr |
The major outer membrane protein OmpU of Vibrio splendidus contributes to host antimicrobial peptide resistance and is required for virulence in the oyster Crassostrea gigas |
title_full_unstemmed |
The major outer membrane protein OmpU of Vibrio splendidus contributes to host antimicrobial peptide resistance and is required for virulence in the oyster Crassostrea gigas |
title_sort |
major outer membrane protein ompu of vibrio splendidus contributes to host antimicrobial peptide resistance and is required for virulence in the oyster crassostrea gigas |
publisher |
Wiley-blackwell Publishing, Inc |
publishDate |
2010 |
url |
https://archimer.ifremer.fr/doc/00003/11421/8044.pdf https://doi.org/10.1111/j.1462-2920.2009.02138.x https://archimer.ifremer.fr/doc/00003/11421/ |
long_lat |
ENVELOPE(-45.900,-45.900,-60.633,-60.633) |
geographic |
Meier |
geographic_facet |
Meier |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Environmental Microbiology (1462-2912) (Wiley-blackwell Publishing, Inc), 2010-04 , Vol. 12 , N. 4 , P. 951-963 |
op_relation |
https://archimer.ifremer.fr/doc/00003/11421/8044.pdf doi:10.1111/j.1462-2920.2009.02138.x https://archimer.ifremer.fr/doc/00003/11421/ |
op_rights |
2010 Society for Applied Microbiology and Blackwell Publishing Ltd info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.1111/j.1462-2920.2009.02138.x |
container_title |
Environmental Microbiology |
container_volume |
12 |
container_issue |
4 |
container_start_page |
951 |
op_container_end_page |
963 |
_version_ |
1766393981476798464 |