Application of staining techniques to improve the viability assessment of turbot (Psetta maxima) ova

Staining-dye procedures were tested on the turbot ovule in order to develop a rapid technique for determining ovule viability. Neutral red and Trypan blue dyes provided better assessment of the proportion of viable or dead female gametes when inducing fertilisation.

Bibliographic Details
Main Authors: Omnes, Marie-helene, Dorange, Germaine, Suquet, Marc, Normant, Yvon
Format: Conference Object
Language:English
Published: Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, July 4-9 1999 1999
Subjects:
Turbot
Online Access:https://archimer.ifremer.fr/doc/00002/11323/7860.pdf
https://archimer.ifremer.fr/doc/00002/11323/
Description
Summary:Staining-dye procedures were tested on the turbot ovule in order to develop a rapid technique for determining ovule viability. Neutral red and Trypan blue dyes provided better assessment of the proportion of viable or dead female gametes when inducing fertilisation.

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