Nitrogen fixation in the widely distributed marine γ-proteobacterial diazotroph Candidatus Thalassolituus haligoni
The high diversity and global distribution of heterotrophic bacterial diazotrophs (HBDs) in the ocean has recently become apparent. However, understanding the role these largely uncultured microorganisms play in marine N 2 fixation poses a challenge due to their undefined growth requirements and the...
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American Association for the Advancement of Science (AAAS)
2024
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ftarchimer:oai:archimer.ifremer.fr:101473 2024-10-13T14:04:59+00:00 Nitrogen fixation in the widely distributed marine γ-proteobacterial diazotroph Candidatus Thalassolituus haligoni Rose, Sonja A. Robicheau, Brent M. Tolman, Jennifer Fonseca-batista, Debany Rowland, Elden Desai, Dhwani Ratten, Jenni-marie Kantor, Ella Joy H. Comeau, André M. Langille, Morgan G.i. Jerlström-hultqvist, Jon Devred, Emmanuel Sarthou, Geraldine Bertrand, Erin M. Laroche, Julie 2024-07 application/pdf https://archimer.ifremer.fr/doc/00903/101473/112290.pdf https://archimer.ifremer.fr/doc/00903/101473/112293.pdf https://archimer.ifremer.fr/doc/00903/101473/112294.zip https://doi.org/10.1126/sciadv.adn1476 https://archimer.ifremer.fr/doc/00903/101473/ eng eng American Association for the Advancement of Science (AAAS) https://archimer.ifremer.fr/doc/00903/101473/112290.pdf https://archimer.ifremer.fr/doc/00903/101473/112293.pdf https://archimer.ifremer.fr/doc/00903/101473/112294.zip doi:10.1126/sciadv.adn1476 https://archimer.ifremer.fr/doc/00903/101473/ info:eu-repo/semantics/openAccess restricted use Science Advances (2375-2548) (American Association for the Advancement of Science (AAAS)), 2024-07 , Vol. 10 , N. 31 , P. eadn1476 (17p.) text Article info:eu-repo/semantics/article 2024 ftarchimer https://doi.org/10.1126/sciadv.adn1476 2024-09-18T00:09:41Z The high diversity and global distribution of heterotrophic bacterial diazotrophs (HBDs) in the ocean has recently become apparent. However, understanding the role these largely uncultured microorganisms play in marine N 2 fixation poses a challenge due to their undefined growth requirements and the complex regulation of the nitrogenase enzyme. We isolated and characterized Candidatus Thalassolituus haligoni, a member of a widely distributed clade of HBD belonging to the Oceanospirillales. Analysis of its nifH gene via amplicon sequencing revealed the extensive distribution of Cand. T. haligoni across the Pacific, Atlantic, and Arctic Oceans. Pangenome analysis indicates that the isolate shares >99% identity with an uncultured metagenome-assembled genome called Arc-Gamma-03, recently recovered from the Arctic Ocean. Through combined genomic, proteomic, and physiological approaches, we confirmed that the isolate fixes N 2 gas. However, the mechanisms governing nitrogenase regulation in Cand. T. haligoni remain unclear. We propose Cand. T. haligoni as a globally distributed, cultured HBD model species within this understudied clade of Oceanospirillales. Article in Journal/Newspaper Arctic Arctic Ocean Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Arctic Arctic Ocean Pacific Science Advances 10 31 |
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Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
description |
The high diversity and global distribution of heterotrophic bacterial diazotrophs (HBDs) in the ocean has recently become apparent. However, understanding the role these largely uncultured microorganisms play in marine N 2 fixation poses a challenge due to their undefined growth requirements and the complex regulation of the nitrogenase enzyme. We isolated and characterized Candidatus Thalassolituus haligoni, a member of a widely distributed clade of HBD belonging to the Oceanospirillales. Analysis of its nifH gene via amplicon sequencing revealed the extensive distribution of Cand. T. haligoni across the Pacific, Atlantic, and Arctic Oceans. Pangenome analysis indicates that the isolate shares >99% identity with an uncultured metagenome-assembled genome called Arc-Gamma-03, recently recovered from the Arctic Ocean. Through combined genomic, proteomic, and physiological approaches, we confirmed that the isolate fixes N 2 gas. However, the mechanisms governing nitrogenase regulation in Cand. T. haligoni remain unclear. We propose Cand. T. haligoni as a globally distributed, cultured HBD model species within this understudied clade of Oceanospirillales. |
format |
Article in Journal/Newspaper |
author |
Rose, Sonja A. Robicheau, Brent M. Tolman, Jennifer Fonseca-batista, Debany Rowland, Elden Desai, Dhwani Ratten, Jenni-marie Kantor, Ella Joy H. Comeau, André M. Langille, Morgan G.i. Jerlström-hultqvist, Jon Devred, Emmanuel Sarthou, Geraldine Bertrand, Erin M. Laroche, Julie |
spellingShingle |
Rose, Sonja A. Robicheau, Brent M. Tolman, Jennifer Fonseca-batista, Debany Rowland, Elden Desai, Dhwani Ratten, Jenni-marie Kantor, Ella Joy H. Comeau, André M. Langille, Morgan G.i. Jerlström-hultqvist, Jon Devred, Emmanuel Sarthou, Geraldine Bertrand, Erin M. Laroche, Julie Nitrogen fixation in the widely distributed marine γ-proteobacterial diazotroph Candidatus Thalassolituus haligoni |
author_facet |
Rose, Sonja A. Robicheau, Brent M. Tolman, Jennifer Fonseca-batista, Debany Rowland, Elden Desai, Dhwani Ratten, Jenni-marie Kantor, Ella Joy H. Comeau, André M. Langille, Morgan G.i. Jerlström-hultqvist, Jon Devred, Emmanuel Sarthou, Geraldine Bertrand, Erin M. Laroche, Julie |
author_sort |
Rose, Sonja A. |
title |
Nitrogen fixation in the widely distributed marine γ-proteobacterial diazotroph Candidatus Thalassolituus haligoni |
title_short |
Nitrogen fixation in the widely distributed marine γ-proteobacterial diazotroph Candidatus Thalassolituus haligoni |
title_full |
Nitrogen fixation in the widely distributed marine γ-proteobacterial diazotroph Candidatus Thalassolituus haligoni |
title_fullStr |
Nitrogen fixation in the widely distributed marine γ-proteobacterial diazotroph Candidatus Thalassolituus haligoni |
title_full_unstemmed |
Nitrogen fixation in the widely distributed marine γ-proteobacterial diazotroph Candidatus Thalassolituus haligoni |
title_sort |
nitrogen fixation in the widely distributed marine γ-proteobacterial diazotroph candidatus thalassolituus haligoni |
publisher |
American Association for the Advancement of Science (AAAS) |
publishDate |
2024 |
url |
https://archimer.ifremer.fr/doc/00903/101473/112290.pdf https://archimer.ifremer.fr/doc/00903/101473/112293.pdf https://archimer.ifremer.fr/doc/00903/101473/112294.zip https://doi.org/10.1126/sciadv.adn1476 https://archimer.ifremer.fr/doc/00903/101473/ |
geographic |
Arctic Arctic Ocean Pacific |
geographic_facet |
Arctic Arctic Ocean Pacific |
genre |
Arctic Arctic Ocean |
genre_facet |
Arctic Arctic Ocean |
op_source |
Science Advances (2375-2548) (American Association for the Advancement of Science (AAAS)), 2024-07 , Vol. 10 , N. 31 , P. eadn1476 (17p.) |
op_relation |
https://archimer.ifremer.fr/doc/00903/101473/112290.pdf https://archimer.ifremer.fr/doc/00903/101473/112293.pdf https://archimer.ifremer.fr/doc/00903/101473/112294.zip doi:10.1126/sciadv.adn1476 https://archimer.ifremer.fr/doc/00903/101473/ |
op_rights |
info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.1126/sciadv.adn1476 |
container_title |
Science Advances |
container_volume |
10 |
container_issue |
31 |
_version_ |
1812810814082514944 |