Xenotransplantation of European Eel (Anguilla anguilla) Spermatogonia in Zebrafish (Danio rerio) and European Sea Bass (Dicentrarchus labrax)
The European eel encounters challenges in achieving sexual maturation in captivity, which has been a concern for researchers. This study explores surrogate broodstock technology as an alternative approach for eel production. The present study aimed to evaluate zebrafish and European sea bass as pote...
Published in: | Fishes |
---|---|
Main Authors: | , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
MDPI AG
2024
|
Subjects: | |
Online Access: | https://archimer.ifremer.fr/doc/00901/101275/111884.pdf https://archimer.ifremer.fr/doc/00901/101275/111885.zip https://doi.org/10.3390/fishes9070290 https://archimer.ifremer.fr/doc/00901/101275/ |
id |
ftarchimer:oai:archimer.ifremer.fr:101275 |
---|---|
record_format |
openpolar |
spelling |
ftarchimer:oai:archimer.ifremer.fr:101275 2024-09-15T17:39:42+00:00 Xenotransplantation of European Eel (Anguilla anguilla) Spermatogonia in Zebrafish (Danio rerio) and European Sea Bass (Dicentrarchus labrax) Blanes-garcía, Marta Marinović, Zoran Morini, Marina Vergnet, Alain Horváth, Ákos Asturiano, Juan F. 2024-07 application/pdf https://archimer.ifremer.fr/doc/00901/101275/111884.pdf https://archimer.ifremer.fr/doc/00901/101275/111885.zip https://doi.org/10.3390/fishes9070290 https://archimer.ifremer.fr/doc/00901/101275/ eng eng MDPI AG https://archimer.ifremer.fr/doc/00901/101275/111884.pdf https://archimer.ifremer.fr/doc/00901/101275/111885.zip doi:10.3390/fishes9070290 https://archimer.ifremer.fr/doc/00901/101275/ info:eu-repo/semantics/openAccess restricted use Fishes (2410-3888) (MDPI AG), 2024-07 , Vol. 9 , N. 7 , P. 290 (21p.) transplantation surrogate production surrogacy spermatogonial stem cells testis cryopreservation text Article info:eu-repo/semantics/article 2024 ftarchimer https://doi.org/10.3390/fishes9070290 2024-08-13T23:39:38Z The European eel encounters challenges in achieving sexual maturation in captivity, which has been a concern for researchers. This study explores surrogate broodstock technology as an alternative approach for eel production. The present study aimed to evaluate zebrafish and European sea bass as potential recipients for European eel spermatogonia transplantation, given the abundance of eel type A spermatogonia (SPGA). Immature European eel testes were dissected and maintained at 4 °C or cryopreserved. SPGA were obtained by dissociation of fresh or post-thawed tissue, employing an enzymatic solution, and then labelled with fluorescent membrane marker PKH26. SPGA from fresh tissue were transplanted into wild-type zebrafish larvae and triploid European sea bass larvae, while SPGA from cryopreserved testis were transplanted into vasa::egfp transgenic zebrafish larvae. One-and-a-half months post-transplantation (mpt), fluorescent donor cells were not detected in the gonads of zebrafish or European sea bass. Molecular qPCR analyses at 1.5 or 6 mpt did not reveal European eel-specific gene expression in the gonads of any transplanted fish. The findings suggest that the gonadal microenvironments of zebrafish and European sea bass are unsuitable for the development of European eel spermatogonia, highlighting distinctive spermatogonial stem cell migration mechanisms within teleost species Article in Journal/Newspaper Anguilla anguilla European eel Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Fishes 9 7 290 |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
transplantation surrogate production surrogacy spermatogonial stem cells testis cryopreservation |
spellingShingle |
transplantation surrogate production surrogacy spermatogonial stem cells testis cryopreservation Blanes-garcía, Marta Marinović, Zoran Morini, Marina Vergnet, Alain Horváth, Ákos Asturiano, Juan F. Xenotransplantation of European Eel (Anguilla anguilla) Spermatogonia in Zebrafish (Danio rerio) and European Sea Bass (Dicentrarchus labrax) |
topic_facet |
transplantation surrogate production surrogacy spermatogonial stem cells testis cryopreservation |
description |
The European eel encounters challenges in achieving sexual maturation in captivity, which has been a concern for researchers. This study explores surrogate broodstock technology as an alternative approach for eel production. The present study aimed to evaluate zebrafish and European sea bass as potential recipients for European eel spermatogonia transplantation, given the abundance of eel type A spermatogonia (SPGA). Immature European eel testes were dissected and maintained at 4 °C or cryopreserved. SPGA were obtained by dissociation of fresh or post-thawed tissue, employing an enzymatic solution, and then labelled with fluorescent membrane marker PKH26. SPGA from fresh tissue were transplanted into wild-type zebrafish larvae and triploid European sea bass larvae, while SPGA from cryopreserved testis were transplanted into vasa::egfp transgenic zebrafish larvae. One-and-a-half months post-transplantation (mpt), fluorescent donor cells were not detected in the gonads of zebrafish or European sea bass. Molecular qPCR analyses at 1.5 or 6 mpt did not reveal European eel-specific gene expression in the gonads of any transplanted fish. The findings suggest that the gonadal microenvironments of zebrafish and European sea bass are unsuitable for the development of European eel spermatogonia, highlighting distinctive spermatogonial stem cell migration mechanisms within teleost species |
format |
Article in Journal/Newspaper |
author |
Blanes-garcía, Marta Marinović, Zoran Morini, Marina Vergnet, Alain Horváth, Ákos Asturiano, Juan F. |
author_facet |
Blanes-garcía, Marta Marinović, Zoran Morini, Marina Vergnet, Alain Horváth, Ákos Asturiano, Juan F. |
author_sort |
Blanes-garcía, Marta |
title |
Xenotransplantation of European Eel (Anguilla anguilla) Spermatogonia in Zebrafish (Danio rerio) and European Sea Bass (Dicentrarchus labrax) |
title_short |
Xenotransplantation of European Eel (Anguilla anguilla) Spermatogonia in Zebrafish (Danio rerio) and European Sea Bass (Dicentrarchus labrax) |
title_full |
Xenotransplantation of European Eel (Anguilla anguilla) Spermatogonia in Zebrafish (Danio rerio) and European Sea Bass (Dicentrarchus labrax) |
title_fullStr |
Xenotransplantation of European Eel (Anguilla anguilla) Spermatogonia in Zebrafish (Danio rerio) and European Sea Bass (Dicentrarchus labrax) |
title_full_unstemmed |
Xenotransplantation of European Eel (Anguilla anguilla) Spermatogonia in Zebrafish (Danio rerio) and European Sea Bass (Dicentrarchus labrax) |
title_sort |
xenotransplantation of european eel (anguilla anguilla) spermatogonia in zebrafish (danio rerio) and european sea bass (dicentrarchus labrax) |
publisher |
MDPI AG |
publishDate |
2024 |
url |
https://archimer.ifremer.fr/doc/00901/101275/111884.pdf https://archimer.ifremer.fr/doc/00901/101275/111885.zip https://doi.org/10.3390/fishes9070290 https://archimer.ifremer.fr/doc/00901/101275/ |
genre |
Anguilla anguilla European eel |
genre_facet |
Anguilla anguilla European eel |
op_source |
Fishes (2410-3888) (MDPI AG), 2024-07 , Vol. 9 , N. 7 , P. 290 (21p.) |
op_relation |
https://archimer.ifremer.fr/doc/00901/101275/111884.pdf https://archimer.ifremer.fr/doc/00901/101275/111885.zip doi:10.3390/fishes9070290 https://archimer.ifremer.fr/doc/00901/101275/ |
op_rights |
info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.3390/fishes9070290 |
container_title |
Fishes |
container_volume |
9 |
container_issue |
7 |
container_start_page |
290 |
_version_ |
1810481882033291264 |