Morpho- and Chemotyping of Holopelagic Sargassum Species Causing Massive Strandings in the Caribbean Region
The specific identification of three major morphotypes of the tropical holopelagic Sargassum species causing massive strandings on the African and Caribbean coastlines was attempted by morphological characterisation as well as quantitative and qualitative analyses of several metabolites. Of the 25 m...
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ftarchimer:oai:archimer.ifremer.fr:100957 2024-09-15T18:23:35+00:00 Morpho- and Chemotyping of Holopelagic Sargassum Species Causing Massive Strandings in the Caribbean Region Kergosien, Nolwenn Helias, Mathieu Le Grand, Fabienne Cerantola, Stéphane Simon, Gaelle Nirma, Charlotte Thibaut, Thierry Berline, Léo Changeux, Thomas Blanfumé, Aurélie Connan, Solène Stiger-pouvreau, Valerie 2024-07 application/pdf https://archimer.ifremer.fr/doc/00898/100957/111465.pdf https://archimer.ifremer.fr/doc/00898/100957/111466.zip https://doi.org/10.3390/phycology4030018 https://archimer.ifremer.fr/doc/00898/100957/ eng eng MDPI https://archimer.ifremer.fr/doc/00898/100957/111465.pdf https://archimer.ifremer.fr/doc/00898/100957/111466.zip doi:10.3390/phycology4030018 https://archimer.ifremer.fr/doc/00898/100957/ info:eu-repo/semantics/openAccess restricted use Phycology (2673-9410) (MDPI), 2024-07 , Vol. 4 , N. 3 , P. 340-362 chemotyping fatty acids holopelagic Sargassum morphotyping NMR fingerprinting phlorotannins pigments text Article info:eu-repo/semantics/article 2024 ftarchimer https://doi.org/10.3390/phycology4030018 2024-07-09T23:44:06Z The specific identification of three major morphotypes of the tropical holopelagic Sargassum species causing massive strandings on the African and Caribbean coastlines was attempted by morphological characterisation as well as quantitative and qualitative analyses of several metabolites. Of the 25 morphological variables studied on 208 samples from the North Atlantic Ocean, 22 were used to establish a dichotomous identification key, allowing without any doubt the identification of each morphotype based on their morphological criteria alone. We also attempted to differentiate morphotypes using chemical fingerprintings (HR-MAS NMR) and markers by analysing pigment level and composition using High Pressure Liquid Chromatography, terpene profiles by Thin Layer Chromatography, phenolic compound levels by the Folin-Ciocalteu assay and structures by 2D Nuclear Magnetic Resonance spectroscopy, and fatty acid composition by Gas Chromatography. While pigment level and composition, terpene profiles, and phenolic contents were not discriminating, quantification of eight fatty acids enabled the differentiation of the three morphotypes. Furthermore, phlorotannin purification permitted their structural characterisation allowing discrimination between the three morphotypes. Our study highlights the potential of the free fatty acid profile and phlorotannin structure as good chemomarkers in order to discriminate between the three morphotypes of holopelagic Sargassum. Article in Journal/Newspaper North Atlantic Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) Phycology 4 3 340 362 |
institution |
Open Polar |
collection |
Archimer (Archive Institutionnelle de l'Ifremer - Institut français de recherche pour l'exploitation de la mer) |
op_collection_id |
ftarchimer |
language |
English |
topic |
chemotyping fatty acids holopelagic Sargassum morphotyping NMR fingerprinting phlorotannins pigments |
spellingShingle |
chemotyping fatty acids holopelagic Sargassum morphotyping NMR fingerprinting phlorotannins pigments Kergosien, Nolwenn Helias, Mathieu Le Grand, Fabienne Cerantola, Stéphane Simon, Gaelle Nirma, Charlotte Thibaut, Thierry Berline, Léo Changeux, Thomas Blanfumé, Aurélie Connan, Solène Stiger-pouvreau, Valerie Morpho- and Chemotyping of Holopelagic Sargassum Species Causing Massive Strandings in the Caribbean Region |
topic_facet |
chemotyping fatty acids holopelagic Sargassum morphotyping NMR fingerprinting phlorotannins pigments |
description |
The specific identification of three major morphotypes of the tropical holopelagic Sargassum species causing massive strandings on the African and Caribbean coastlines was attempted by morphological characterisation as well as quantitative and qualitative analyses of several metabolites. Of the 25 morphological variables studied on 208 samples from the North Atlantic Ocean, 22 were used to establish a dichotomous identification key, allowing without any doubt the identification of each morphotype based on their morphological criteria alone. We also attempted to differentiate morphotypes using chemical fingerprintings (HR-MAS NMR) and markers by analysing pigment level and composition using High Pressure Liquid Chromatography, terpene profiles by Thin Layer Chromatography, phenolic compound levels by the Folin-Ciocalteu assay and structures by 2D Nuclear Magnetic Resonance spectroscopy, and fatty acid composition by Gas Chromatography. While pigment level and composition, terpene profiles, and phenolic contents were not discriminating, quantification of eight fatty acids enabled the differentiation of the three morphotypes. Furthermore, phlorotannin purification permitted their structural characterisation allowing discrimination between the three morphotypes. Our study highlights the potential of the free fatty acid profile and phlorotannin structure as good chemomarkers in order to discriminate between the three morphotypes of holopelagic Sargassum. |
format |
Article in Journal/Newspaper |
author |
Kergosien, Nolwenn Helias, Mathieu Le Grand, Fabienne Cerantola, Stéphane Simon, Gaelle Nirma, Charlotte Thibaut, Thierry Berline, Léo Changeux, Thomas Blanfumé, Aurélie Connan, Solène Stiger-pouvreau, Valerie |
author_facet |
Kergosien, Nolwenn Helias, Mathieu Le Grand, Fabienne Cerantola, Stéphane Simon, Gaelle Nirma, Charlotte Thibaut, Thierry Berline, Léo Changeux, Thomas Blanfumé, Aurélie Connan, Solène Stiger-pouvreau, Valerie |
author_sort |
Kergosien, Nolwenn |
title |
Morpho- and Chemotyping of Holopelagic Sargassum Species Causing Massive Strandings in the Caribbean Region |
title_short |
Morpho- and Chemotyping of Holopelagic Sargassum Species Causing Massive Strandings in the Caribbean Region |
title_full |
Morpho- and Chemotyping of Holopelagic Sargassum Species Causing Massive Strandings in the Caribbean Region |
title_fullStr |
Morpho- and Chemotyping of Holopelagic Sargassum Species Causing Massive Strandings in the Caribbean Region |
title_full_unstemmed |
Morpho- and Chemotyping of Holopelagic Sargassum Species Causing Massive Strandings in the Caribbean Region |
title_sort |
morpho- and chemotyping of holopelagic sargassum species causing massive strandings in the caribbean region |
publisher |
MDPI |
publishDate |
2024 |
url |
https://archimer.ifremer.fr/doc/00898/100957/111465.pdf https://archimer.ifremer.fr/doc/00898/100957/111466.zip https://doi.org/10.3390/phycology4030018 https://archimer.ifremer.fr/doc/00898/100957/ |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_source |
Phycology (2673-9410) (MDPI), 2024-07 , Vol. 4 , N. 3 , P. 340-362 |
op_relation |
https://archimer.ifremer.fr/doc/00898/100957/111465.pdf https://archimer.ifremer.fr/doc/00898/100957/111466.zip doi:10.3390/phycology4030018 https://archimer.ifremer.fr/doc/00898/100957/ |
op_rights |
info:eu-repo/semantics/openAccess restricted use |
op_doi |
https://doi.org/10.3390/phycology4030018 |
container_title |
Phycology |
container_volume |
4 |
container_issue |
3 |
container_start_page |
340 |
op_container_end_page |
362 |
_version_ |
1810463811807739904 |