Microsatellite primers for red drum (Sciaenops ocellatus)
In this note, we document polymerase-chain-reaction (PCR) primer pairs for 101 nuclear-encoded microsatellites designed and developed from a genomic library for red drum (Sciaenops ocellatus). Details of the genomic library construction, the sequencing of positive clones, primer design, and PCR prot...
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ftaquaticcommons:oai:generic.eprints.org:8828 2023-05-15T18:06:00+02:00 Microsatellite primers for red drum (Sciaenops ocellatus) Karlsson, Sten Renshaw, Mark A. Rexroad III, Caird E. Gold, John R. 2008 application/pdf http://aquaticcommons.org/8828/ http://fishbull.noaa.gov/1064/karlsson.pdf http://aquaticcommons.org/8828/1/karlsson_Fish_Bull_2008.pdf en eng http://aquaticcommons.org/8828/1/karlsson_Fish_Bull_2008.pdf Karlsson, Sten and Renshaw, Mark A. and Rexroad III, Caird E. and Gold, John R. (2008) Microsatellite primers for red drum (Sciaenops ocellatus). Fishery Bulletin, 106(4), pp. 476-482. Biology Ecology Fisheries Article PeerReviewed 2008 ftaquaticcommons 2020-02-27T09:23:14Z In this note, we document polymerase-chain-reaction (PCR) primer pairs for 101 nuclear-encoded microsatellites designed and developed from a genomic library for red drum (Sciaenops ocellatus). Details of the genomic library construction, the sequencing of positive clones, primer design, and PCR protocols may be found in Karlsson et al. (2008). The 101 microsatellites (GENBA NK Accession Numbers EU015882-EU015982) were amplified successfully and used to genotype 24 red drum obtained from Galveston Bay, Texas (Table 1). A total of 69 of the microsatellites had an uninterrupted (perfect) dinucleotide motif, and 30 had an imperfect dinucleotide motif; one microsatellite had an imperfect tetranucleotide motif, and one had an imperfect and compound motif (Table 1 ). Sizes of the cloned alleles ranged from 84 to 252 base pairs. A ‘blast’ search of the GENBANK database indicated that all of the primers and the cloned alleles were unique (i.e., not duplicated). Article in Journal/Newspaper Red drum Sciaenops ocellatus International Association of Aquatic and Marine Science Libraries and Information Centers (IAMSLIC): Aquatic Commons |
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Open Polar |
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International Association of Aquatic and Marine Science Libraries and Information Centers (IAMSLIC): Aquatic Commons |
op_collection_id |
ftaquaticcommons |
language |
English |
topic |
Biology Ecology Fisheries |
spellingShingle |
Biology Ecology Fisheries Karlsson, Sten Renshaw, Mark A. Rexroad III, Caird E. Gold, John R. Microsatellite primers for red drum (Sciaenops ocellatus) |
topic_facet |
Biology Ecology Fisheries |
description |
In this note, we document polymerase-chain-reaction (PCR) primer pairs for 101 nuclear-encoded microsatellites designed and developed from a genomic library for red drum (Sciaenops ocellatus). Details of the genomic library construction, the sequencing of positive clones, primer design, and PCR protocols may be found in Karlsson et al. (2008). The 101 microsatellites (GENBA NK Accession Numbers EU015882-EU015982) were amplified successfully and used to genotype 24 red drum obtained from Galveston Bay, Texas (Table 1). A total of 69 of the microsatellites had an uninterrupted (perfect) dinucleotide motif, and 30 had an imperfect dinucleotide motif; one microsatellite had an imperfect tetranucleotide motif, and one had an imperfect and compound motif (Table 1 ). Sizes of the cloned alleles ranged from 84 to 252 base pairs. A ‘blast’ search of the GENBANK database indicated that all of the primers and the cloned alleles were unique (i.e., not duplicated). |
format |
Article in Journal/Newspaper |
author |
Karlsson, Sten Renshaw, Mark A. Rexroad III, Caird E. Gold, John R. |
author_facet |
Karlsson, Sten Renshaw, Mark A. Rexroad III, Caird E. Gold, John R. |
author_sort |
Karlsson, Sten |
title |
Microsatellite primers for red drum (Sciaenops ocellatus) |
title_short |
Microsatellite primers for red drum (Sciaenops ocellatus) |
title_full |
Microsatellite primers for red drum (Sciaenops ocellatus) |
title_fullStr |
Microsatellite primers for red drum (Sciaenops ocellatus) |
title_full_unstemmed |
Microsatellite primers for red drum (Sciaenops ocellatus) |
title_sort |
microsatellite primers for red drum (sciaenops ocellatus) |
publishDate |
2008 |
url |
http://aquaticcommons.org/8828/ http://fishbull.noaa.gov/1064/karlsson.pdf http://aquaticcommons.org/8828/1/karlsson_Fish_Bull_2008.pdf |
genre |
Red drum Sciaenops ocellatus |
genre_facet |
Red drum Sciaenops ocellatus |
op_relation |
http://aquaticcommons.org/8828/1/karlsson_Fish_Bull_2008.pdf Karlsson, Sten and Renshaw, Mark A. and Rexroad III, Caird E. and Gold, John R. (2008) Microsatellite primers for red drum (Sciaenops ocellatus). Fishery Bulletin, 106(4), pp. 476-482. |
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