Creating a sperm bank from sturgeon brood stocks in the south Caspian Sea
Sturgeon stocks are drastically decreasing in Caspian Sea during recent decades. This project has been proposed to collect the excess sperm of Caspian Sea Southern part sturgeon breeders, to cryopreserve and hold them for further use. During two spawning seasons (2006-2007), sperm quality and quanti...
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ftaquaticcommons:oai:generic.eprints.org:25397 2023-05-15T15:41:48+02:00 Creating a sperm bank from sturgeon brood stocks in the south Caspian Sea Baradaran Noveiri, Shahrouz Alipoor, A.R. Mohseni, M. Pordehghani, M. Chakmedooz, F. Halajiyan, A. 2012 application/pdf http://aquaticcommons.org/25397/ http://aquaticcommons.org/25397/1/40607.pdf fa per Iranian Fisheries Science Research Institute http://aquaticcommons.org/25397/1/40607.pdf Baradaran Noveiri, Shahrouz and Alipoor, A.R. and Mohseni, M. and Pordehghani, M. and Chakmedooz, F. and Halajiyan, A. (2012) Creating a sperm bank from sturgeon brood stocks in the south Caspian Sea. Tehran, Iran, Iranian Fisheries Science Research Institute, 50pp. (40607). Aquaculture Monograph or Serial Issue NonPeerReviewed 2012 ftaquaticcommons 2020-02-27T09:32:26Z Sturgeon stocks are drastically decreasing in Caspian Sea during recent decades. This project has been proposed to collect the excess sperm of Caspian Sea Southern part sturgeon breeders, to cryopreserve and hold them for further use. During two spawning seasons (2006-2007), sperm quality and quantity analysis were conducted on 34 different male sturgeons of which 12 Persian sturgeon, 4 stellate sturgeon, 1 beluga and 1 ship sturgeon with better quality were chosen to cryopreserve. After dilution of fresh sperm with sturgeon sperm diluent (115 mM Tris, 23.4 mM sucrose and 15% DMSO) at the ratio of 1:1, the 0.5 ml straws were filled using special filling and sealing apparatus and frozen with a multi step freezing rate. To access the further quality and fertilization test, samples were thawed at 40 °C for 20 seconds. During the project performance, 1010 ml of Persian sturgeon sperm , 110 ml of ship sturgeon sperm , 90 ml of stellate and 80 ml of beluga sperm were cryopreserved. The reduction of frozen-thawed sperm motility in comparison with fresh samples showed 10-52.3 % decrease, but there was not any significant differences between samples after 7 months preservation in liquid Nitrogen (P < 0.005). River caught Persian sturgeon samples showed higher sperm density in comparison with nonriverine breeders (P < 0.05). Data showed that stellate sturgeon sperm showed better resistance to freezing condition in comparison with Persian sturgeon spermatozoa. Fertilization tests (4 hours after fertilization) showed 27.4% and 58.2% fertilization rate in frozen-thawed stellate and Persian sturgeon spermatozoa, respectively. Book Beluga Beluga* International Association of Aquatic and Marine Science Libraries and Information Centers (IAMSLIC): Aquatic Commons |
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Open Polar |
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International Association of Aquatic and Marine Science Libraries and Information Centers (IAMSLIC): Aquatic Commons |
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ftaquaticcommons |
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Persian |
topic |
Aquaculture |
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Aquaculture Baradaran Noveiri, Shahrouz Alipoor, A.R. Mohseni, M. Pordehghani, M. Chakmedooz, F. Halajiyan, A. Creating a sperm bank from sturgeon brood stocks in the south Caspian Sea |
topic_facet |
Aquaculture |
description |
Sturgeon stocks are drastically decreasing in Caspian Sea during recent decades. This project has been proposed to collect the excess sperm of Caspian Sea Southern part sturgeon breeders, to cryopreserve and hold them for further use. During two spawning seasons (2006-2007), sperm quality and quantity analysis were conducted on 34 different male sturgeons of which 12 Persian sturgeon, 4 stellate sturgeon, 1 beluga and 1 ship sturgeon with better quality were chosen to cryopreserve. After dilution of fresh sperm with sturgeon sperm diluent (115 mM Tris, 23.4 mM sucrose and 15% DMSO) at the ratio of 1:1, the 0.5 ml straws were filled using special filling and sealing apparatus and frozen with a multi step freezing rate. To access the further quality and fertilization test, samples were thawed at 40 °C for 20 seconds. During the project performance, 1010 ml of Persian sturgeon sperm , 110 ml of ship sturgeon sperm , 90 ml of stellate and 80 ml of beluga sperm were cryopreserved. The reduction of frozen-thawed sperm motility in comparison with fresh samples showed 10-52.3 % decrease, but there was not any significant differences between samples after 7 months preservation in liquid Nitrogen (P < 0.005). River caught Persian sturgeon samples showed higher sperm density in comparison with nonriverine breeders (P < 0.05). Data showed that stellate sturgeon sperm showed better resistance to freezing condition in comparison with Persian sturgeon spermatozoa. Fertilization tests (4 hours after fertilization) showed 27.4% and 58.2% fertilization rate in frozen-thawed stellate and Persian sturgeon spermatozoa, respectively. |
format |
Book |
author |
Baradaran Noveiri, Shahrouz Alipoor, A.R. Mohseni, M. Pordehghani, M. Chakmedooz, F. Halajiyan, A. |
author_facet |
Baradaran Noveiri, Shahrouz Alipoor, A.R. Mohseni, M. Pordehghani, M. Chakmedooz, F. Halajiyan, A. |
author_sort |
Baradaran Noveiri, Shahrouz |
title |
Creating a sperm bank from sturgeon brood stocks in the south Caspian Sea |
title_short |
Creating a sperm bank from sturgeon brood stocks in the south Caspian Sea |
title_full |
Creating a sperm bank from sturgeon brood stocks in the south Caspian Sea |
title_fullStr |
Creating a sperm bank from sturgeon brood stocks in the south Caspian Sea |
title_full_unstemmed |
Creating a sperm bank from sturgeon brood stocks in the south Caspian Sea |
title_sort |
creating a sperm bank from sturgeon brood stocks in the south caspian sea |
publisher |
Iranian Fisheries Science Research Institute |
publishDate |
2012 |
url |
http://aquaticcommons.org/25397/ http://aquaticcommons.org/25397/1/40607.pdf |
genre |
Beluga Beluga* |
genre_facet |
Beluga Beluga* |
op_relation |
http://aquaticcommons.org/25397/1/40607.pdf Baradaran Noveiri, Shahrouz and Alipoor, A.R. and Mohseni, M. and Pordehghani, M. and Chakmedooz, F. and Halajiyan, A. (2012) Creating a sperm bank from sturgeon brood stocks in the south Caspian Sea. Tehran, Iran, Iranian Fisheries Science Research Institute, 50pp. (40607). |
_version_ |
1766374683910864896 |