Identification of bester hybrids (female Huso huso Linnaeus, 1758 and male sterlet Acipenser ruthenus Linnaeus, 1758) using AFLP molecular technique
In this study Amplified Fragment Length Polymorphism (AFLP) was applied to species identification of bester hybrids. Hybrids identification was performed by comparison of electrophoresis profiles with parental species. The simultaneous occurrence of diagnostic bands fixed in the parental species, ge...
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ftaquaticcommons:oai:generic.eprints.org:22524 2023-05-15T15:41:50+02:00 Identification of bester hybrids (female Huso huso Linnaeus, 1758 and male sterlet Acipenser ruthenus Linnaeus, 1758) using AFLP molecular technique Yarmohammadi, M. Shabani, A. Pourkazemi, M. Baradaran Noveiri, S. 2012 application/pdf http://aquaticcommons.org/22524/ http://aquaticcommons.org/22524/1/IFRO-v11n2p415-en.pdf en eng http://aquaticcommons.org/22524/1/IFRO-v11n2p415-en.pdf Yarmohammadi, M. and Shabani, A. and Pourkazemi, M. and Baradaran Noveiri, S. (2012) Identification of bester hybrids (female Huso huso Linnaeus, 1758 and male sterlet Acipenser ruthenus Linnaeus, 1758) using AFLP molecular technique. Iranian Journal of Fisheries Sciences, 11(2), pp. 415-423. Biology Fisheries Article PeerReviewed 2012 ftaquaticcommons 2020-02-27T09:30:40Z In this study Amplified Fragment Length Polymorphism (AFLP) was applied to species identification of bester hybrids. Hybrids identification was performed by comparison of electrophoresis profiles with parental species. The simultaneous occurrence of diagnostic bands fixed in the parental species, genetic distance and identification and cluster analyses (UPGMA) allow a correct identification. We used 8 primer combinations (Eco+3, Mse+4) and a total of 250 bands (size range 40-1000 bp) were generated. Primer combinations of (E-AAT, M-CGAT) and (E-AAG, M-CGAT) produced diagnostic bands in hybrids and parental species. Moreover, the results of genetic identification showed that Bester hybrids are more similar to beluga (Huso huso) (0.68) in comparison with sterlet (Acipenser ruthenus) (0.45).The results suggested that this technique could be suitable for precise identification of species and inter-generic hybrids like bester. Article in Journal/Newspaper Beluga Beluga* International Association of Aquatic and Marine Science Libraries and Information Centers (IAMSLIC): Aquatic Commons |
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International Association of Aquatic and Marine Science Libraries and Information Centers (IAMSLIC): Aquatic Commons |
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ftaquaticcommons |
language |
English |
topic |
Biology Fisheries |
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Biology Fisheries Yarmohammadi, M. Shabani, A. Pourkazemi, M. Baradaran Noveiri, S. Identification of bester hybrids (female Huso huso Linnaeus, 1758 and male sterlet Acipenser ruthenus Linnaeus, 1758) using AFLP molecular technique |
topic_facet |
Biology Fisheries |
description |
In this study Amplified Fragment Length Polymorphism (AFLP) was applied to species identification of bester hybrids. Hybrids identification was performed by comparison of electrophoresis profiles with parental species. The simultaneous occurrence of diagnostic bands fixed in the parental species, genetic distance and identification and cluster analyses (UPGMA) allow a correct identification. We used 8 primer combinations (Eco+3, Mse+4) and a total of 250 bands (size range 40-1000 bp) were generated. Primer combinations of (E-AAT, M-CGAT) and (E-AAG, M-CGAT) produced diagnostic bands in hybrids and parental species. Moreover, the results of genetic identification showed that Bester hybrids are more similar to beluga (Huso huso) (0.68) in comparison with sterlet (Acipenser ruthenus) (0.45).The results suggested that this technique could be suitable for precise identification of species and inter-generic hybrids like bester. |
format |
Article in Journal/Newspaper |
author |
Yarmohammadi, M. Shabani, A. Pourkazemi, M. Baradaran Noveiri, S. |
author_facet |
Yarmohammadi, M. Shabani, A. Pourkazemi, M. Baradaran Noveiri, S. |
author_sort |
Yarmohammadi, M. |
title |
Identification of bester hybrids (female Huso huso Linnaeus, 1758 and male sterlet Acipenser ruthenus Linnaeus, 1758) using AFLP molecular technique |
title_short |
Identification of bester hybrids (female Huso huso Linnaeus, 1758 and male sterlet Acipenser ruthenus Linnaeus, 1758) using AFLP molecular technique |
title_full |
Identification of bester hybrids (female Huso huso Linnaeus, 1758 and male sterlet Acipenser ruthenus Linnaeus, 1758) using AFLP molecular technique |
title_fullStr |
Identification of bester hybrids (female Huso huso Linnaeus, 1758 and male sterlet Acipenser ruthenus Linnaeus, 1758) using AFLP molecular technique |
title_full_unstemmed |
Identification of bester hybrids (female Huso huso Linnaeus, 1758 and male sterlet Acipenser ruthenus Linnaeus, 1758) using AFLP molecular technique |
title_sort |
identification of bester hybrids (female huso huso linnaeus, 1758 and male sterlet acipenser ruthenus linnaeus, 1758) using aflp molecular technique |
publishDate |
2012 |
url |
http://aquaticcommons.org/22524/ http://aquaticcommons.org/22524/1/IFRO-v11n2p415-en.pdf |
genre |
Beluga Beluga* |
genre_facet |
Beluga Beluga* |
op_relation |
http://aquaticcommons.org/22524/1/IFRO-v11n2p415-en.pdf Yarmohammadi, M. and Shabani, A. and Pourkazemi, M. and Baradaran Noveiri, S. (2012) Identification of bester hybrids (female Huso huso Linnaeus, 1758 and male sterlet Acipenser ruthenus Linnaeus, 1758) using AFLP molecular technique. Iranian Journal of Fisheries Sciences, 11(2), pp. 415-423. |
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1766374720269189120 |