Searching the genome of beluga (Huso huso) for sex markers based on targeted bulked segregant analysis (BSA)
In sturgeon aquaculture, where the main purpose is caviar production, a reliable method is needed to separate fish according to gender. Currently, due to the lack of external sexual dimorphism, the fish are sexed by an invasive surgical examination of the gonads. Development of a non-invasive proced...
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ftaquaticcommons:oai:generic.eprints.org:21740 2023-05-15T15:41:44+02:00 Searching the genome of beluga (Huso huso) for sex markers based on targeted bulked segregant analysis (BSA) Khodaparast, M. Keyvanshokooh, S. Pourkazemi, M. Hosseini, S.J. Zolgharnein, H. 2014 application/pdf http://aquaticcommons.org/21740/ http://aquaticcommons.org/21740/1/2 --- 013-84-5.pdf en eng http://aquaticcommons.org/21740/1/2 --- 013-84-5.pdf Khodaparast, M. and Keyvanshokooh, S. and Pourkazemi, M. and Hosseini, S.J. and Zolgharnein, H. (2014) Searching the genome of beluga (Huso huso) for sex markers based on targeted bulked segregant analysis (BSA). Caspian Journal of Environmental Sciences, 12(2), pp. 185-195. Biology Fisheries Article PeerReviewed 2014 ftaquaticcommons 2020-02-27T09:30:10Z In sturgeon aquaculture, where the main purpose is caviar production, a reliable method is needed to separate fish according to gender. Currently, due to the lack of external sexual dimorphism, the fish are sexed by an invasive surgical examination of the gonads. Development of a non-invasive procedure for sexing fish based on genetic markers is of special interest. In the present study we employed Bulked Segregant Analysis (BSA) methodology to search for DNA markers associated with the sex of the beluga sturgeon (Huso huso). DNA bulks (male and female) were created by combining equal amounts of genomic DNA from 10 fish of both sexes. A total of 101 decamer primers associated with the sex-specific sequences in non-sturgeon species was used for targeted screening of the bulks, resulting in 2846 bands that all of them were present in both sexes. Our results showed that sex chromosomes are weakly differentiated in the sturgeon genome and comprised sequences not complementary to the sex-specific primers in non-sturgeon species. Article in Journal/Newspaper Beluga Beluga* International Association of Aquatic and Marine Science Libraries and Information Centers (IAMSLIC): Aquatic Commons |
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International Association of Aquatic and Marine Science Libraries and Information Centers (IAMSLIC): Aquatic Commons |
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ftaquaticcommons |
language |
English |
topic |
Biology Fisheries |
spellingShingle |
Biology Fisheries Khodaparast, M. Keyvanshokooh, S. Pourkazemi, M. Hosseini, S.J. Zolgharnein, H. Searching the genome of beluga (Huso huso) for sex markers based on targeted bulked segregant analysis (BSA) |
topic_facet |
Biology Fisheries |
description |
In sturgeon aquaculture, where the main purpose is caviar production, a reliable method is needed to separate fish according to gender. Currently, due to the lack of external sexual dimorphism, the fish are sexed by an invasive surgical examination of the gonads. Development of a non-invasive procedure for sexing fish based on genetic markers is of special interest. In the present study we employed Bulked Segregant Analysis (BSA) methodology to search for DNA markers associated with the sex of the beluga sturgeon (Huso huso). DNA bulks (male and female) were created by combining equal amounts of genomic DNA from 10 fish of both sexes. A total of 101 decamer primers associated with the sex-specific sequences in non-sturgeon species was used for targeted screening of the bulks, resulting in 2846 bands that all of them were present in both sexes. Our results showed that sex chromosomes are weakly differentiated in the sturgeon genome and comprised sequences not complementary to the sex-specific primers in non-sturgeon species. |
format |
Article in Journal/Newspaper |
author |
Khodaparast, M. Keyvanshokooh, S. Pourkazemi, M. Hosseini, S.J. Zolgharnein, H. |
author_facet |
Khodaparast, M. Keyvanshokooh, S. Pourkazemi, M. Hosseini, S.J. Zolgharnein, H. |
author_sort |
Khodaparast, M. |
title |
Searching the genome of beluga (Huso huso) for sex markers based on targeted bulked segregant analysis (BSA) |
title_short |
Searching the genome of beluga (Huso huso) for sex markers based on targeted bulked segregant analysis (BSA) |
title_full |
Searching the genome of beluga (Huso huso) for sex markers based on targeted bulked segregant analysis (BSA) |
title_fullStr |
Searching the genome of beluga (Huso huso) for sex markers based on targeted bulked segregant analysis (BSA) |
title_full_unstemmed |
Searching the genome of beluga (Huso huso) for sex markers based on targeted bulked segregant analysis (BSA) |
title_sort |
searching the genome of beluga (huso huso) for sex markers based on targeted bulked segregant analysis (bsa) |
publishDate |
2014 |
url |
http://aquaticcommons.org/21740/ http://aquaticcommons.org/21740/1/2 --- 013-84-5.pdf |
genre |
Beluga Beluga* |
genre_facet |
Beluga Beluga* |
op_relation |
http://aquaticcommons.org/21740/1/2 --- 013-84-5.pdf Khodaparast, M. and Keyvanshokooh, S. and Pourkazemi, M. and Hosseini, S.J. and Zolgharnein, H. (2014) Searching the genome of beluga (Huso huso) for sex markers based on targeted bulked segregant analysis (BSA). Caspian Journal of Environmental Sciences, 12(2), pp. 185-195. |
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1766374633450242048 |