Blood transcriptomes and de novo identification of candidate loci for mating success in lekking great snipe ( Gallinago media )
We assembled the great snipe blood transcriptome using data from fourteen lekking males, in order to de novo identify candidate genes related to sexual selection, and determined the expression profiles in relation to mating success. The three most highly transcribed genes were encoding different hae...
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ftanucanberra:oai:openresearch-repository.anu.edu.au:1885/233024 2024-01-14T10:06:59+01:00 Blood transcriptomes and de novo identification of candidate loci for mating success in lekking great snipe ( Gallinago media ) Hoglund, Jacob Wang, Biao Sæther, Stein Are Blom, Mozes Fiske, Peder Halvarsson, Peter Horsburgh, Gavin J Burke, Terry Kalas, John Atle Ekblom, Robert application/pdf http://hdl.handle.net/1885/233024 https://doi.org/10.1111/mec.14118 https://openresearch-repository.anu.edu.au/bitstream/1885/233024/3/01_Hoglund_Blood_transcriptomes_and_de_2017.pdf.jpg en_AU eng Blackwell Publishing Ltd 0962-1083 http://hdl.handle.net/1885/233024 doi:10.1111/mec.14118 https://openresearch-repository.anu.edu.au/bitstream/1885/233024/3/01_Hoglund_Blood_transcriptomes_and_de_2017.pdf.jpg © 2017 John Wiley & Sons Ltd Molecular Ecology adaptation birds sexual selection transcriptomics Journal article ftanucanberra https://doi.org/10.1111/mec.14118 2023-12-15T09:37:05Z We assembled the great snipe blood transcriptome using data from fourteen lekking males, in order to de novo identify candidate genes related to sexual selection, and determined the expression profiles in relation to mating success. The three most highly transcribed genes were encoding different haemoglobin subunits. All tended to be overexpressed in males with high mating success. We also called single nucleotide polymorphisms (SNPs) from the transcriptome data and found considerable genetic variation for many genes expressed during lekking. Among these, we identified 14 polymorphic candidate SNPs that had a significant genotypic association with mating success (number of females mated with) and/or mating status (mated or not). Four of the candidate SNPs were found in HBAA (encoding the haemoglobin α-chain). Heterozygotes for one of these and one SNP in the gene PABPC1 appeared to enjoy higher mating success compared to males homozygous for either of the alleles. In a larger data set of individuals, we genotyped 38 of the identified SNPs but found low support for consistent selection as only one of the zygosities of previously identified candidate SNPs and none of their genotypes were associated with mating status. However, candidate SNPs generally showed lower levels of spatial genetic structure compared to noncandidate markers. We also scored the prevalence of avian malaria in a subsample of birds. Males infected with avian malaria parasites had lower mating success in the year of sampling than noninfected males. Parasite infection and its interaction with specific genes may thus affect performance on the lek Carl Trygger’s Foundation, VR (Swedish Research Council); UK Natural Environment Research Council; Carl Trygger Foundation; Swedish Research Council Article in Journal/Newspaper Gallinago media great snipe Australian National University: ANU Digital Collections Molecular Ecology 26 13 3458 3471 |
institution |
Open Polar |
collection |
Australian National University: ANU Digital Collections |
op_collection_id |
ftanucanberra |
language |
English |
topic |
adaptation birds sexual selection transcriptomics |
spellingShingle |
adaptation birds sexual selection transcriptomics Hoglund, Jacob Wang, Biao Sæther, Stein Are Blom, Mozes Fiske, Peder Halvarsson, Peter Horsburgh, Gavin J Burke, Terry Kalas, John Atle Ekblom, Robert Blood transcriptomes and de novo identification of candidate loci for mating success in lekking great snipe ( Gallinago media ) |
topic_facet |
adaptation birds sexual selection transcriptomics |
description |
We assembled the great snipe blood transcriptome using data from fourteen lekking males, in order to de novo identify candidate genes related to sexual selection, and determined the expression profiles in relation to mating success. The three most highly transcribed genes were encoding different haemoglobin subunits. All tended to be overexpressed in males with high mating success. We also called single nucleotide polymorphisms (SNPs) from the transcriptome data and found considerable genetic variation for many genes expressed during lekking. Among these, we identified 14 polymorphic candidate SNPs that had a significant genotypic association with mating success (number of females mated with) and/or mating status (mated or not). Four of the candidate SNPs were found in HBAA (encoding the haemoglobin α-chain). Heterozygotes for one of these and one SNP in the gene PABPC1 appeared to enjoy higher mating success compared to males homozygous for either of the alleles. In a larger data set of individuals, we genotyped 38 of the identified SNPs but found low support for consistent selection as only one of the zygosities of previously identified candidate SNPs and none of their genotypes were associated with mating status. However, candidate SNPs generally showed lower levels of spatial genetic structure compared to noncandidate markers. We also scored the prevalence of avian malaria in a subsample of birds. Males infected with avian malaria parasites had lower mating success in the year of sampling than noninfected males. Parasite infection and its interaction with specific genes may thus affect performance on the lek Carl Trygger’s Foundation, VR (Swedish Research Council); UK Natural Environment Research Council; Carl Trygger Foundation; Swedish Research Council |
format |
Article in Journal/Newspaper |
author |
Hoglund, Jacob Wang, Biao Sæther, Stein Are Blom, Mozes Fiske, Peder Halvarsson, Peter Horsburgh, Gavin J Burke, Terry Kalas, John Atle Ekblom, Robert |
author_facet |
Hoglund, Jacob Wang, Biao Sæther, Stein Are Blom, Mozes Fiske, Peder Halvarsson, Peter Horsburgh, Gavin J Burke, Terry Kalas, John Atle Ekblom, Robert |
author_sort |
Hoglund, Jacob |
title |
Blood transcriptomes and de novo identification of candidate loci for mating success in lekking great snipe ( Gallinago media ) |
title_short |
Blood transcriptomes and de novo identification of candidate loci for mating success in lekking great snipe ( Gallinago media ) |
title_full |
Blood transcriptomes and de novo identification of candidate loci for mating success in lekking great snipe ( Gallinago media ) |
title_fullStr |
Blood transcriptomes and de novo identification of candidate loci for mating success in lekking great snipe ( Gallinago media ) |
title_full_unstemmed |
Blood transcriptomes and de novo identification of candidate loci for mating success in lekking great snipe ( Gallinago media ) |
title_sort |
blood transcriptomes and de novo identification of candidate loci for mating success in lekking great snipe ( gallinago media ) |
publisher |
Blackwell Publishing Ltd |
url |
http://hdl.handle.net/1885/233024 https://doi.org/10.1111/mec.14118 https://openresearch-repository.anu.edu.au/bitstream/1885/233024/3/01_Hoglund_Blood_transcriptomes_and_de_2017.pdf.jpg |
genre |
Gallinago media great snipe |
genre_facet |
Gallinago media great snipe |
op_source |
Molecular Ecology |
op_relation |
0962-1083 http://hdl.handle.net/1885/233024 doi:10.1111/mec.14118 https://openresearch-repository.anu.edu.au/bitstream/1885/233024/3/01_Hoglund_Blood_transcriptomes_and_de_2017.pdf.jpg |
op_rights |
© 2017 John Wiley & Sons Ltd |
op_doi |
https://doi.org/10.1111/mec.14118 |
container_title |
Molecular Ecology |
container_volume |
26 |
container_issue |
13 |
container_start_page |
3458 |
op_container_end_page |
3471 |
_version_ |
1788061399099899904 |