Cloning and analysis of transcripts and genes encoding fish-specific proteins related to PrP
We report cloning and tissue expression of transcripts of a fourth fish-specific gene (SPRNB) encoding a prion protein (PrP)-related protein (Shadoo2) from two species, Cyprinus carpio (carp) and Danio rerio (zebrafish), as well as gene or partial transcript sequences identified by in silico searche...
Published in: | Fish Physiology and Biochemistry |
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ftanucanberra:oai:openresearch-repository.anu.edu.au:1885/23293 2024-01-14T10:10:19+01:00 Cloning and analysis of transcripts and genes encoding fish-specific proteins related to PrP Strumbo, B Sangiorgio, Lorenzo Ronchi, S Gready, Jill Simonic, Tatjana http://hdl.handle.net/1885/23293 https://doi.org/10.1007/s10695-006-9108-3 https://openresearch-repository.anu.edu.au/bitstream/1885/23293/5/StrumboSangiorgio.pdf.jpg https://openresearch-repository.anu.edu.au/bitstream/1885/23293/7/01_Strumbo_Cloning_and_analysis_of_2006.pdf.jpg unknown Kluwer Academic Publishers 0920-1742 http://hdl.handle.net/1885/23293 doi:10.1007/s10695-006-9108-3 https://openresearch-repository.anu.edu.au/bitstream/1885/23293/5/StrumboSangiorgio.pdf.jpg https://openresearch-repository.anu.edu.au/bitstream/1885/23293/7/01_Strumbo_Cloning_and_analysis_of_2006.pdf.jpg Fish Physiology and Biochemistry Keywords: complementary DNA messenger RNA prion protein prion protein 1 prion protein 2 protein derivative protein subunit Shadoo2 protein unclassified drug 3' untranslated region 5' untranslated region article carp computer model controlled study DN cDNA cloning Fish-specific PrP-related proteins mRNA expression Journal article ftanucanberra https://doi.org/10.1007/s10695-006-9108-3 2023-12-15T09:37:30Z We report cloning and tissue expression of transcripts of a fourth fish-specific gene (SPRNB) encoding a prion protein (PrP)-related protein (Shadoo2) from two species, Cyprinus carpio (carp) and Danio rerio (zebrafish), as well as gene or partial transcript sequences identified by in silico searches of public databases from Gasterosteus aculeatus (stickleback), Oryzias latipes (medaka fish) and Salmo salar (salmon). We also present an up-to-date summary of current knowledge of the other three PrP-related genes and transcripts (PrP-like protein, PrP1 and PrP2) encoded exclusively in fish genomes, including some additional transcripts we identified by in silico searches. We compare levels of protein-sequence conservation among the different genes within given fish species, and among given genes in different fish species, and analyse the gene structures and contexts (flanking genes) where genomic sequence is available. These analyses show remarkably high divergences of sequences and expression patterns for proteins in distant fish, such as Fugu and zebrafish, leaving open the question of how varied their functions may be. Article in Journal/Newspaper Salmo salar Australian National University: ANU Digital Collections Fish Physiology and Biochemistry 32 4 339 353 |
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Open Polar |
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Australian National University: ANU Digital Collections |
op_collection_id |
ftanucanberra |
language |
unknown |
topic |
Keywords: complementary DNA messenger RNA prion protein prion protein 1 prion protein 2 protein derivative protein subunit Shadoo2 protein unclassified drug 3' untranslated region 5' untranslated region article carp computer model controlled study DN cDNA cloning Fish-specific PrP-related proteins mRNA expression |
spellingShingle |
Keywords: complementary DNA messenger RNA prion protein prion protein 1 prion protein 2 protein derivative protein subunit Shadoo2 protein unclassified drug 3' untranslated region 5' untranslated region article carp computer model controlled study DN cDNA cloning Fish-specific PrP-related proteins mRNA expression Strumbo, B Sangiorgio, Lorenzo Ronchi, S Gready, Jill Simonic, Tatjana Cloning and analysis of transcripts and genes encoding fish-specific proteins related to PrP |
topic_facet |
Keywords: complementary DNA messenger RNA prion protein prion protein 1 prion protein 2 protein derivative protein subunit Shadoo2 protein unclassified drug 3' untranslated region 5' untranslated region article carp computer model controlled study DN cDNA cloning Fish-specific PrP-related proteins mRNA expression |
description |
We report cloning and tissue expression of transcripts of a fourth fish-specific gene (SPRNB) encoding a prion protein (PrP)-related protein (Shadoo2) from two species, Cyprinus carpio (carp) and Danio rerio (zebrafish), as well as gene or partial transcript sequences identified by in silico searches of public databases from Gasterosteus aculeatus (stickleback), Oryzias latipes (medaka fish) and Salmo salar (salmon). We also present an up-to-date summary of current knowledge of the other three PrP-related genes and transcripts (PrP-like protein, PrP1 and PrP2) encoded exclusively in fish genomes, including some additional transcripts we identified by in silico searches. We compare levels of protein-sequence conservation among the different genes within given fish species, and among given genes in different fish species, and analyse the gene structures and contexts (flanking genes) where genomic sequence is available. These analyses show remarkably high divergences of sequences and expression patterns for proteins in distant fish, such as Fugu and zebrafish, leaving open the question of how varied their functions may be. |
format |
Article in Journal/Newspaper |
author |
Strumbo, B Sangiorgio, Lorenzo Ronchi, S Gready, Jill Simonic, Tatjana |
author_facet |
Strumbo, B Sangiorgio, Lorenzo Ronchi, S Gready, Jill Simonic, Tatjana |
author_sort |
Strumbo, B |
title |
Cloning and analysis of transcripts and genes encoding fish-specific proteins related to PrP |
title_short |
Cloning and analysis of transcripts and genes encoding fish-specific proteins related to PrP |
title_full |
Cloning and analysis of transcripts and genes encoding fish-specific proteins related to PrP |
title_fullStr |
Cloning and analysis of transcripts and genes encoding fish-specific proteins related to PrP |
title_full_unstemmed |
Cloning and analysis of transcripts and genes encoding fish-specific proteins related to PrP |
title_sort |
cloning and analysis of transcripts and genes encoding fish-specific proteins related to prp |
publisher |
Kluwer Academic Publishers |
url |
http://hdl.handle.net/1885/23293 https://doi.org/10.1007/s10695-006-9108-3 https://openresearch-repository.anu.edu.au/bitstream/1885/23293/5/StrumboSangiorgio.pdf.jpg https://openresearch-repository.anu.edu.au/bitstream/1885/23293/7/01_Strumbo_Cloning_and_analysis_of_2006.pdf.jpg |
genre |
Salmo salar |
genre_facet |
Salmo salar |
op_source |
Fish Physiology and Biochemistry |
op_relation |
0920-1742 http://hdl.handle.net/1885/23293 doi:10.1007/s10695-006-9108-3 https://openresearch-repository.anu.edu.au/bitstream/1885/23293/5/StrumboSangiorgio.pdf.jpg https://openresearch-repository.anu.edu.au/bitstream/1885/23293/7/01_Strumbo_Cloning_and_analysis_of_2006.pdf.jpg |
op_doi |
https://doi.org/10.1007/s10695-006-9108-3 |
container_title |
Fish Physiology and Biochemistry |
container_volume |
32 |
container_issue |
4 |
container_start_page |
339 |
op_container_end_page |
353 |
_version_ |
1788064999506182144 |