DNA sequencing and genetic diversity of the 18S-26S nuclear ribosomal internal transcribed spacers (ITS) in nine Antarctic moss species
We have sequenced the 18S-26S nuclear ribosomal DNA ITS region from the genome of nine different moss species from the Ross Sea region of Antarctica. This relatively quick and simple technique enables these species to be readily distinguished, facilitating their taxonomic identification. Only a sing...
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British Antarctic Survey
2015
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ftanucanberra:oai:digitalcollections.anu.edu.au:1885/84791 2023-05-15T13:35:18+02:00 DNA sequencing and genetic diversity of the 18S-26S nuclear ribosomal internal transcribed spacers (ITS) in nine Antarctic moss species Skotnicki, Mary Mackenzie, Anne Clements, M A Selkirk, Patricia M 2015-12-13T23:02:13Z http://hdl.handle.net/1885/84791 unknown British Antarctic Survey 0954-1020 http://hdl.handle.net/1885/84791 Antarctic Science Keywords: molecular ecology moss Antarctica Arctic and Antarctic World Bryophyta bryophytes Bryophyte ITS sequence Molecular ecology Molecular taxonomy Moss dispersal Victoria Land Journal article 2015 ftanucanberra 2015-12-28T23:38:41Z We have sequenced the 18S-26S nuclear ribosomal DNA ITS region from the genome of nine different moss species from the Ross Sea region of Antarctica. This relatively quick and simple technique enables these species to be readily distinguished, facilitating their taxonomic identification. Only a single moss shoot is required, and for identification of these bryophytes it is only necessary to determine a few hundred nucleotides of the DNA sequence in a single sequencing reaction. Several previously unidentified Antarctic moss specimens were readily characterized by comparison with ITS sequences of known moss species. The relationships between species and locations previously detected by the RAPD (Random Amplified Polymorphic DNA) technique were confirmed by DNA sequencing, demonstrating that the two techniques can be complementary for molecular analysis of the ecology of mosses in Antarctica. Article in Journal/Newspaper Antarc* Antarctic Antarctic Science Antarctica Arctic Ross Sea Victoria Land Australian National University: ANU Digital Collections Antarctic Arctic Ross Sea Victoria Land |
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Australian National University: ANU Digital Collections |
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ftanucanberra |
language |
unknown |
topic |
Keywords: molecular ecology moss Antarctica Arctic and Antarctic World Bryophyta bryophytes Bryophyte ITS sequence Molecular ecology Molecular taxonomy Moss dispersal Victoria Land |
spellingShingle |
Keywords: molecular ecology moss Antarctica Arctic and Antarctic World Bryophyta bryophytes Bryophyte ITS sequence Molecular ecology Molecular taxonomy Moss dispersal Victoria Land Skotnicki, Mary Mackenzie, Anne Clements, M A Selkirk, Patricia M DNA sequencing and genetic diversity of the 18S-26S nuclear ribosomal internal transcribed spacers (ITS) in nine Antarctic moss species |
topic_facet |
Keywords: molecular ecology moss Antarctica Arctic and Antarctic World Bryophyta bryophytes Bryophyte ITS sequence Molecular ecology Molecular taxonomy Moss dispersal Victoria Land |
description |
We have sequenced the 18S-26S nuclear ribosomal DNA ITS region from the genome of nine different moss species from the Ross Sea region of Antarctica. This relatively quick and simple technique enables these species to be readily distinguished, facilitating their taxonomic identification. Only a single moss shoot is required, and for identification of these bryophytes it is only necessary to determine a few hundred nucleotides of the DNA sequence in a single sequencing reaction. Several previously unidentified Antarctic moss specimens were readily characterized by comparison with ITS sequences of known moss species. The relationships between species and locations previously detected by the RAPD (Random Amplified Polymorphic DNA) technique were confirmed by DNA sequencing, demonstrating that the two techniques can be complementary for molecular analysis of the ecology of mosses in Antarctica. |
format |
Article in Journal/Newspaper |
author |
Skotnicki, Mary Mackenzie, Anne Clements, M A Selkirk, Patricia M |
author_facet |
Skotnicki, Mary Mackenzie, Anne Clements, M A Selkirk, Patricia M |
author_sort |
Skotnicki, Mary |
title |
DNA sequencing and genetic diversity of the 18S-26S nuclear ribosomal internal transcribed spacers (ITS) in nine Antarctic moss species |
title_short |
DNA sequencing and genetic diversity of the 18S-26S nuclear ribosomal internal transcribed spacers (ITS) in nine Antarctic moss species |
title_full |
DNA sequencing and genetic diversity of the 18S-26S nuclear ribosomal internal transcribed spacers (ITS) in nine Antarctic moss species |
title_fullStr |
DNA sequencing and genetic diversity of the 18S-26S nuclear ribosomal internal transcribed spacers (ITS) in nine Antarctic moss species |
title_full_unstemmed |
DNA sequencing and genetic diversity of the 18S-26S nuclear ribosomal internal transcribed spacers (ITS) in nine Antarctic moss species |
title_sort |
dna sequencing and genetic diversity of the 18s-26s nuclear ribosomal internal transcribed spacers (its) in nine antarctic moss species |
publisher |
British Antarctic Survey |
publishDate |
2015 |
url |
http://hdl.handle.net/1885/84791 |
geographic |
Antarctic Arctic Ross Sea Victoria Land |
geographic_facet |
Antarctic Arctic Ross Sea Victoria Land |
genre |
Antarc* Antarctic Antarctic Science Antarctica Arctic Ross Sea Victoria Land |
genre_facet |
Antarc* Antarctic Antarctic Science Antarctica Arctic Ross Sea Victoria Land |
op_source |
Antarctic Science |
op_relation |
0954-1020 http://hdl.handle.net/1885/84791 |
_version_ |
1766063974663585792 |