Tracking changes in bioavailable Fe within high-nitrate low-chlorophyll oceanic waters: A first estimate using a heterotrophic bacterial bioreporter
It is conventional knowledge that heterotrophic bacteria play a key role in the biogeochemical cycling of oceanic carbon. However, only recently has their role in marine iron (Fe) biogeochemical cycles been examined. Research during this past decade has demonstrated an inextricable link between Fe c...
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ftanucanberra:oai:digitalcollections.anu.edu.au:1885/28454 2023-05-15T13:57:36+02:00 Tracking changes in bioavailable Fe within high-nitrate low-chlorophyll oceanic waters: A first estimate using a heterotrophic bacterial bioreporter Mioni, C Handy, S Ellwood, Michael Twiss, Michael R McKay, R M Boyd, P W Wilhelm, S W 2015-12-07T22:55:35Z http://hdl.handle.net/1885/28454 unknown American Geophysical Union 0886-6236 http://hdl.handle.net/1885/28454 Global Biogeochemical Cycles Keywords: Bacteria Biology Bioluminescence Coastal zones Ecosystems Geochemistry Iron Seawater bacterium bioavailability biogeochemical cycle biological uptake estimation method nitrate Bacteria (microorganisms) Journal article 2015 ftanucanberra 2015-12-28T23:22:26Z It is conventional knowledge that heterotrophic bacteria play a key role in the biogeochemical cycling of oceanic carbon. However, only recently has their role in marine iron (Fe) biogeochemical cycles been examined. Research during this past decade has demonstrated an inextricable link between Fe chemistry and the biota, as >99% of Fe in marine systems is complexed to organic chelates of unknown but obviously biotic origin. Here we present a novel approach to assess and compare Fe bioavailability in low Fe HNLC waters using a bioluminescent bacterial reporter that quantitatively responds to the concentration of bioavailable Fe by producing light. Originally tested in freshwater environments, this study presents the first characterization of this halotolerant reporter organism in a defined seawater medium and then subsequently in marine surface waters. Laboratory characterizations demonstrate that this reporter displays a dose-dependent response to Fe availability in our defined marine medium. Field tests were performed during the 10-day mesoscale FeCycle experiment (February 2003) in the Pacific sub-Antarctic high-nitrate low-chlorophyll region. Data from both biogeochemical. measures and bioreporter assays are provided which describe how the bioreporter detected changes in Fe bioavailability that occurred during a natural shift in ambient dissolved Fe concentrations (∼40 pM). Our data explore the use of heterotrophic bioluminescent reporters as a comparable tool for marine ecosystems and demonstrate the potential utility of this tool in elucidating the relationship between Fe bioavailability and Fe chemistry in complex marine systems. Article in Journal/Newspaper Antarc* Antarctic Australian National University: ANU Digital Collections Antarctic Pacific |
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Open Polar |
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Australian National University: ANU Digital Collections |
op_collection_id |
ftanucanberra |
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unknown |
topic |
Keywords: Bacteria Biology Bioluminescence Coastal zones Ecosystems Geochemistry Iron Seawater bacterium bioavailability biogeochemical cycle biological uptake estimation method nitrate Bacteria (microorganisms) |
spellingShingle |
Keywords: Bacteria Biology Bioluminescence Coastal zones Ecosystems Geochemistry Iron Seawater bacterium bioavailability biogeochemical cycle biological uptake estimation method nitrate Bacteria (microorganisms) Mioni, C Handy, S Ellwood, Michael Twiss, Michael R McKay, R M Boyd, P W Wilhelm, S W Tracking changes in bioavailable Fe within high-nitrate low-chlorophyll oceanic waters: A first estimate using a heterotrophic bacterial bioreporter |
topic_facet |
Keywords: Bacteria Biology Bioluminescence Coastal zones Ecosystems Geochemistry Iron Seawater bacterium bioavailability biogeochemical cycle biological uptake estimation method nitrate Bacteria (microorganisms) |
description |
It is conventional knowledge that heterotrophic bacteria play a key role in the biogeochemical cycling of oceanic carbon. However, only recently has their role in marine iron (Fe) biogeochemical cycles been examined. Research during this past decade has demonstrated an inextricable link between Fe chemistry and the biota, as >99% of Fe in marine systems is complexed to organic chelates of unknown but obviously biotic origin. Here we present a novel approach to assess and compare Fe bioavailability in low Fe HNLC waters using a bioluminescent bacterial reporter that quantitatively responds to the concentration of bioavailable Fe by producing light. Originally tested in freshwater environments, this study presents the first characterization of this halotolerant reporter organism in a defined seawater medium and then subsequently in marine surface waters. Laboratory characterizations demonstrate that this reporter displays a dose-dependent response to Fe availability in our defined marine medium. Field tests were performed during the 10-day mesoscale FeCycle experiment (February 2003) in the Pacific sub-Antarctic high-nitrate low-chlorophyll region. Data from both biogeochemical. measures and bioreporter assays are provided which describe how the bioreporter detected changes in Fe bioavailability that occurred during a natural shift in ambient dissolved Fe concentrations (∼40 pM). Our data explore the use of heterotrophic bioluminescent reporters as a comparable tool for marine ecosystems and demonstrate the potential utility of this tool in elucidating the relationship between Fe bioavailability and Fe chemistry in complex marine systems. |
format |
Article in Journal/Newspaper |
author |
Mioni, C Handy, S Ellwood, Michael Twiss, Michael R McKay, R M Boyd, P W Wilhelm, S W |
author_facet |
Mioni, C Handy, S Ellwood, Michael Twiss, Michael R McKay, R M Boyd, P W Wilhelm, S W |
author_sort |
Mioni, C |
title |
Tracking changes in bioavailable Fe within high-nitrate low-chlorophyll oceanic waters: A first estimate using a heterotrophic bacterial bioreporter |
title_short |
Tracking changes in bioavailable Fe within high-nitrate low-chlorophyll oceanic waters: A first estimate using a heterotrophic bacterial bioreporter |
title_full |
Tracking changes in bioavailable Fe within high-nitrate low-chlorophyll oceanic waters: A first estimate using a heterotrophic bacterial bioreporter |
title_fullStr |
Tracking changes in bioavailable Fe within high-nitrate low-chlorophyll oceanic waters: A first estimate using a heterotrophic bacterial bioreporter |
title_full_unstemmed |
Tracking changes in bioavailable Fe within high-nitrate low-chlorophyll oceanic waters: A first estimate using a heterotrophic bacterial bioreporter |
title_sort |
tracking changes in bioavailable fe within high-nitrate low-chlorophyll oceanic waters: a first estimate using a heterotrophic bacterial bioreporter |
publisher |
American Geophysical Union |
publishDate |
2015 |
url |
http://hdl.handle.net/1885/28454 |
geographic |
Antarctic Pacific |
geographic_facet |
Antarctic Pacific |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Global Biogeochemical Cycles |
op_relation |
0886-6236 http://hdl.handle.net/1885/28454 |
_version_ |
1766265303710302208 |