Rapid acclimation of juvenile corals to CO2-mediated acidification by upregulation of heat shock protein and Bcl-2 genes

• Main Authors: Moya, A., Huisman, L., Forêt, S., Gattuso, J.-P., Hayward, David C., Ball, E. E., Miller, D. J.
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: Wiley 2015
• Subjects: acropora millepora; bcl-2; caspases; climate change; corals; heat shock proteins; acclimatization; animals; anthozoa; carbon dioxide; gene expression profiling; heat-shock proteins; high-throughput nucleotide sequencing; hydrogen-ion concentration; multigene family; oxidative stress; queensland; sequence analysis; rna; transcriptome; up-regulation; genes; Queensland; Ocean acidification
• Online Access: http://hdl.handle.net/1885/14926

Corals play a key role in ocean ecosystems and carbonate balance, but their molecular response to ocean acidification remains unclear. The only previous whole-transcriptome study (Moya et al. Molecular Ecology, 2012; 21, 2440) documented extensive disruption of gene expression, particularly of genes encoding skeletal organic matrix proteins, in juvenile corals (Acropora millepora) after short-term (3 d) exposure to elevated pCO2 . In this study, whole-transcriptome analysis was used to compare the effects of such 'acute' (3 d) exposure to elevated pCO2 with a longer ('prolonged'; 9 d) period of exposure beginning immediately post-fertilization. Far fewer genes were differentially expressed under the 9-d treatment, and although the transcriptome data implied wholesale disruption of metabolism and calcification genes in the acute treatment experiment, expression of most genes was at control levels after prolonged treatment. There was little overlap between the genes responding to the acute and prolonged treatments, but heat shock proteins (HSPs) and heat shock factors (HSFs) were over-represented amongst the genes responding to both treatments. Amongst these was an HSP70 gene previously shown to be involved in acclimation to thermal stress in a field population of another acroporid coral. The most obvious feature of the molecular response in the 9-d treatment experiment was the upregulation of five distinct Bcl-2 family members, the majority predicted to be anti-apoptotic. This suggests that an important component of the longer term response to elevated CO2 is suppression of apoptosis. It therefore appears that juvenile A. millepora have the capacity to rapidly acclimate to elevated pCO2 , a process mediated by upregulation of specific HSPs and a suite of Bcl-2 family members. This research was supported by the Australian Research Council through Discovery Grant DP1095343 to D.J.M., E.E.B. and S.F., and via the Centre of Excellence for Coral Reef Studies, and by a Marie Curie International Outgoing Fellowship (grant agreement # PIOF-GA-2008-235142 project title AMICAL) to A.M. This work is a contribution to the ‘European Project on Ocean Acidification’ (EPOCA), which received funding from the European Community's Seventh Framework Program (FP7/2007–2013) under grant agreement # 211384.