Morphological and molecular identification of Cryptocotyle lingua metacercariae isolated from Atlantic cod (Gadus morhua) from Danish seas and whiting (Merlangius merlangus) from the English Channel
International audience Trematode larvae (metacercariae) causing black spot disease occur frequently in gills, fins, skin and the superficial muscle layers of marine fish. Species within the genus Cryptocotyle Lühe, 1899 are frequently associated with this disease. Descriptions of the metacercarial s...
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Online Access: | https://hal.science/hal-03997666 https://hal.science/hal-03997666/document https://hal.science/hal-03997666/file/Duflot2021_Article_MorphologicalAndMolecularIdent.pdf https://doi.org/10.1007/s00436-021-07278-6 |
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ftanses:oai:HAL:hal-03997666v1 2024-01-21T10:04:27+01:00 Morphological and molecular identification of Cryptocotyle lingua metacercariae isolated from Atlantic cod (Gadus morhua) from Danish seas and whiting (Merlangius merlangus) from the English Channel Duflot, Maureen Gay, Mélanie Midelet, Graziella Kania, Per Walter Buchmann, Kurt Laboratoire de sécurité des aliments de Maisons-Alfort (LSAl) Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES) Laboratoire de Boulogne sur mer Anses Région Hauts-de-France MED VET NET Ecole doctorale SMRE Laboratory of Aquatic Pathobiology of the University of Copenhagen 2021-08 https://hal.science/hal-03997666 https://hal.science/hal-03997666/document https://hal.science/hal-03997666/file/Duflot2021_Article_MorphologicalAndMolecularIdent.pdf https://doi.org/10.1007/s00436-021-07278-6 en eng HAL CCSD Springer Verlag (Germany) info:eu-repo/semantics/altIdentifier/doi/10.1007/s00436-021-07278-6 hal-03997666 https://hal.science/hal-03997666 https://hal.science/hal-03997666/document https://hal.science/hal-03997666/file/Duflot2021_Article_MorphologicalAndMolecularIdent.pdf doi:10.1007/s00436-021-07278-6 info:eu-repo/semantics/OpenAccess ISSN: 0932-0113 EISSN: 1432-1955 Parasitology Research https://hal.science/hal-03997666 Parasitology Research, 2021, 120 (10), pp.3417-3427. ⟨10.1007/s00436-021-07278-6⟩ Cryptocotyle Morphological identification Molecular identification Heterophyidae English Channel Whiting Cryptocotyle Morphological identification Molecular identification Heterophyidae English Channel Whiting [SDV]Life Sciences [q-bio] info:eu-repo/semantics/article Journal articles 2021 ftanses https://doi.org/10.1007/s00436-021-07278-6 2023-12-27T17:23:24Z International audience Trematode larvae (metacercariae) causing black spot disease occur frequently in gills, fins, skin and the superficial muscle layers of marine fish. Species within the genus Cryptocotyle Lühe, 1899 are frequently associated with this disease. Descriptions of the metacercarial stage are relatively limited and none has hitherto been reported from fish from the English Channel. The present study reports the morphological and molecular identifications of encysted black spot-inducing parasites from whiting ( Merlangius merlangus ) and Atlantic cod ( Gadus morhua ) caught respectively from the north coast of France (English Channel) and from Danish sea waters. Metacercariae were characterised morphologically based on microscopic observations and molecularly using Sanger sequencing of fragments of the mitochondrial cox1 gene and rDNA ITS region. Morphological data were compared with available data in the literature. Phylogenetic trees including reference sequences were built to confirm morphological and molecular identifications. This survey constitutes the first description of C. lingua metacercariae in the English Channel ecosystems. Article in Journal/Newspaper atlantic cod Gadus morhua Anses: HAL (Agence nationale de sécurité sanitaire de l’alimentation, de l’environnement et du travail) Parasitology Research 120 10 3417 3427 |
institution |
Open Polar |
collection |
Anses: HAL (Agence nationale de sécurité sanitaire de l’alimentation, de l’environnement et du travail) |
op_collection_id |
ftanses |
language |
English |
topic |
Cryptocotyle Morphological identification Molecular identification Heterophyidae English Channel Whiting Cryptocotyle Morphological identification Molecular identification Heterophyidae English Channel Whiting [SDV]Life Sciences [q-bio] |
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Cryptocotyle Morphological identification Molecular identification Heterophyidae English Channel Whiting Cryptocotyle Morphological identification Molecular identification Heterophyidae English Channel Whiting [SDV]Life Sciences [q-bio] Duflot, Maureen Gay, Mélanie Midelet, Graziella Kania, Per Walter Buchmann, Kurt Morphological and molecular identification of Cryptocotyle lingua metacercariae isolated from Atlantic cod (Gadus morhua) from Danish seas and whiting (Merlangius merlangus) from the English Channel |
topic_facet |
Cryptocotyle Morphological identification Molecular identification Heterophyidae English Channel Whiting Cryptocotyle Morphological identification Molecular identification Heterophyidae English Channel Whiting [SDV]Life Sciences [q-bio] |
description |
International audience Trematode larvae (metacercariae) causing black spot disease occur frequently in gills, fins, skin and the superficial muscle layers of marine fish. Species within the genus Cryptocotyle Lühe, 1899 are frequently associated with this disease. Descriptions of the metacercarial stage are relatively limited and none has hitherto been reported from fish from the English Channel. The present study reports the morphological and molecular identifications of encysted black spot-inducing parasites from whiting ( Merlangius merlangus ) and Atlantic cod ( Gadus morhua ) caught respectively from the north coast of France (English Channel) and from Danish sea waters. Metacercariae were characterised morphologically based on microscopic observations and molecularly using Sanger sequencing of fragments of the mitochondrial cox1 gene and rDNA ITS region. Morphological data were compared with available data in the literature. Phylogenetic trees including reference sequences were built to confirm morphological and molecular identifications. This survey constitutes the first description of C. lingua metacercariae in the English Channel ecosystems. |
author2 |
Laboratoire de sécurité des aliments de Maisons-Alfort (LSAl) Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES) Laboratoire de Boulogne sur mer Anses Région Hauts-de-France MED VET NET Ecole doctorale SMRE Laboratory of Aquatic Pathobiology of the University of Copenhagen |
format |
Article in Journal/Newspaper |
author |
Duflot, Maureen Gay, Mélanie Midelet, Graziella Kania, Per Walter Buchmann, Kurt |
author_facet |
Duflot, Maureen Gay, Mélanie Midelet, Graziella Kania, Per Walter Buchmann, Kurt |
author_sort |
Duflot, Maureen |
title |
Morphological and molecular identification of Cryptocotyle lingua metacercariae isolated from Atlantic cod (Gadus morhua) from Danish seas and whiting (Merlangius merlangus) from the English Channel |
title_short |
Morphological and molecular identification of Cryptocotyle lingua metacercariae isolated from Atlantic cod (Gadus morhua) from Danish seas and whiting (Merlangius merlangus) from the English Channel |
title_full |
Morphological and molecular identification of Cryptocotyle lingua metacercariae isolated from Atlantic cod (Gadus morhua) from Danish seas and whiting (Merlangius merlangus) from the English Channel |
title_fullStr |
Morphological and molecular identification of Cryptocotyle lingua metacercariae isolated from Atlantic cod (Gadus morhua) from Danish seas and whiting (Merlangius merlangus) from the English Channel |
title_full_unstemmed |
Morphological and molecular identification of Cryptocotyle lingua metacercariae isolated from Atlantic cod (Gadus morhua) from Danish seas and whiting (Merlangius merlangus) from the English Channel |
title_sort |
morphological and molecular identification of cryptocotyle lingua metacercariae isolated from atlantic cod (gadus morhua) from danish seas and whiting (merlangius merlangus) from the english channel |
publisher |
HAL CCSD |
publishDate |
2021 |
url |
https://hal.science/hal-03997666 https://hal.science/hal-03997666/document https://hal.science/hal-03997666/file/Duflot2021_Article_MorphologicalAndMolecularIdent.pdf https://doi.org/10.1007/s00436-021-07278-6 |
genre |
atlantic cod Gadus morhua |
genre_facet |
atlantic cod Gadus morhua |
op_source |
ISSN: 0932-0113 EISSN: 1432-1955 Parasitology Research https://hal.science/hal-03997666 Parasitology Research, 2021, 120 (10), pp.3417-3427. ⟨10.1007/s00436-021-07278-6⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1007/s00436-021-07278-6 hal-03997666 https://hal.science/hal-03997666 https://hal.science/hal-03997666/document https://hal.science/hal-03997666/file/Duflot2021_Article_MorphologicalAndMolecularIdent.pdf doi:10.1007/s00436-021-07278-6 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1007/s00436-021-07278-6 |
container_title |
Parasitology Research |
container_volume |
120 |
container_issue |
10 |
container_start_page |
3417 |
op_container_end_page |
3427 |
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1788694826773905408 |