Molecular characterization of encephalomyocarditis virus strains isolated from an African elephant and rats in a French zoo

• Published in: Journal of Veterinary Diagnostic Investigation
• Main Authors: Romey, Aurore, Lamglait, Benjamin, Blanchard, Yannick, Touzain, Fabrice, Quenault, Helene, Relmy, Anthony, Zientara, Stéphan, Blaise-Boisseau, Sandra, Bakkali-Kassimi, Labib
• Other Authors: Virologie UMR1161 (VIRO), École nationale vétérinaire d'Alfort (ENVA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Normandie, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), La Réserve Africaine de Sigean, Laboratoire de Ploufragan-Plouzané-Niort ANSES, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 2021
• Subjects: Elephants; Encephalomyocarditis virus; Phylogenetic analysis; Rats; MESH: Encephalomyocarditis virus; MESH: Elephants; MESH: Cardiovirus Infections; MESH: Rodent Diseases; MESH: France; MESH: Rats; [SDV]Life Sciences [q-bio]; Rattus rattus
• Online Access: https://anses.hal.science/anses-03899563; https://doi.org/10.1177/1040638720978389

International audience In November 2013, a fatal encephalomyocarditis virus (EMCV) case in a captive African elephant ( Loxodonta africana) occurred at the Réserve Africaine de Sigean, a zoo in the south of France. Here we report the molecular characterization of the EMCV strains isolated from samples collected from the dead elephant and from 3 rats ( Rattus rattus) captured in the zoo at the same time. The EMCV infection was confirmed by reverse-transcription real-time PCR (RT-rtPCR) and genome sequencing. Complete genome sequencing and sequence alignment indicated that the elephant’s EMCV strain was 98.1–99.9% identical to the rat EMCV isolates at the nucleotide sequence level. Phylogenetic analysis of the ORF, P1, VP1, and 3D sequences revealed that the elephant and rat strains clustered into lineage A of the EMCV 1 group. To our knowledge, molecular characterization of EMCV in France and Europe has not been reported previously in a captive elephant. The full genome analyses of EMCV isolated from an elephant and rats in the same outbreak emphasizes the role of rodents in EMCV introduction and circulation in zoos.