Differentiation and traffic of IgM+ B cells between focal dark spots in skeletal muscle of Atlantic salmon, lymphoid and adipose tissues
International audience Focal dark spots (DS) in farmed Atlantic salmon fillets contain a significant number of B cells as revealed by the high abundance of immunoglobulin (Ig) transcripts in transcriptome data. The immune response in DS remains unknown while they represent a major problem in commerc...
Published in: | Fish & Shellfish Immunology |
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Main Authors: | , , , , , |
Other Authors: | , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
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HAL CCSD
2023
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Subjects: | |
Online Access: | https://hal.science/hal-04190866 https://hal.science/hal-04190866/document https://hal.science/hal-04190866/file/1-s2.0-S1050464823003443-main.pdf https://doi.org/10.1016/j.fsi.2023.108858 |
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ftanrparis:oai:HAL:hal-04190866v1 |
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openpolar |
institution |
Open Polar |
collection |
Portail HAL-ANR (Agence Nationale de la Recherche) |
op_collection_id |
ftanrparis |
language |
English |
topic |
Atlantic salmon B-cell Gene expression Healing IgM repertoire Melanin spots [SDV]Life Sciences [q-bio] |
spellingShingle |
Atlantic salmon B-cell Gene expression Healing IgM repertoire Melanin spots [SDV]Life Sciences [q-bio] Jiménez-Guerrero, Raúl Karlsen, Christian René Boudinot, Pierre Afanasyev, Sergey Mørkøre, Turid Krasnov, Aleksei Differentiation and traffic of IgM+ B cells between focal dark spots in skeletal muscle of Atlantic salmon, lymphoid and adipose tissues |
topic_facet |
Atlantic salmon B-cell Gene expression Healing IgM repertoire Melanin spots [SDV]Life Sciences [q-bio] |
description |
International audience Focal dark spots (DS) in farmed Atlantic salmon fillets contain a significant number of B cells as revealed by the high abundance of immunoglobulin (Ig) transcripts in transcriptome data. The immune response in DS remains unknown while they represent a major problem in commercial aquaculture. Here, we characterized the diversity and clonal composition of B cells in DS. Sixteen gene markers of immune cells and antigen presentation were analyzed with RT-qPCR. All genes expression showed a positive correlation with DS area and intensity. The flatter the DS, the higher the expression of cd28, csfr, ctla, igt, and sigm, the lower expression of cd83 and btla, and the larger the cumulative frequency within DS. The expression of most of the analyzed immune genes, including three Ig types and markers of B cells was lower in DS than in the lymphatic organs, head kidney and spleen, but significantly higher compared to skeletal muscle. High levels of ctla4 and cd28 in DS might indicate the recruitment of T cells. Sequencing of IgM repertoire (Ig-seq) assessed migration of B cells by co-occurrence of identical CDR3 sequences in different tissues. The combination of gene expression and Ig-seq revealed the presence of several stages of B cell differentiation in DS. B cells at the earliest stage, with high ratio of membrane to secretory IgM (migm and sigm), showed minor Ig repertoire overlap with other tissues. Further differentiation stage (increased sigm to migm ratio and high expression of pax5 and cd79) was associated with active movement of B cells from DS towards lymphatic organs and visceral fat. Traffic and expression of immune genes decreased at later stages. These B cells could be involved in a response directed against viruses, pathogenic or opportunistic bacteria in DS. Seven of eight fish were positive for salmon alphavirus, and levels were higher in DS than in unstained muscle. PCR with universal primers to the 16S rRNA gene did not detect bacteria in DS. Although the evolution of DS most ... |
author2 |
Virologie et Immunologie Moléculaires (VIM (UR 0892)) Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) Norwegian Directorate of Fisheries for large-scale Industrial Research; Sechenov Institute of Evolutionary Physiology and Biochemistry of RAS, (075-00967-23-00); Agence Nationale de la Recherche, ANR, (ANR-21-CE35-0019); Norges Miljø- og Biovitenskapelige Universitet, NMBU; Fiskeri - og havbruksnæringens forskningsfond, FHF, (901487) ANR-21-CE35-0019,LipoFishVac,Vaccins liponanoparticulaires mARN et ADN pour les poissons, couplés à des adjuvants immunomodulateurs(2021) |
format |
Article in Journal/Newspaper |
author |
Jiménez-Guerrero, Raúl Karlsen, Christian René Boudinot, Pierre Afanasyev, Sergey Mørkøre, Turid Krasnov, Aleksei |
author_facet |
Jiménez-Guerrero, Raúl Karlsen, Christian René Boudinot, Pierre Afanasyev, Sergey Mørkøre, Turid Krasnov, Aleksei |
author_sort |
Jiménez-Guerrero, Raúl |
title |
Differentiation and traffic of IgM+ B cells between focal dark spots in skeletal muscle of Atlantic salmon, lymphoid and adipose tissues |
title_short |
Differentiation and traffic of IgM+ B cells between focal dark spots in skeletal muscle of Atlantic salmon, lymphoid and adipose tissues |
title_full |
Differentiation and traffic of IgM+ B cells between focal dark spots in skeletal muscle of Atlantic salmon, lymphoid and adipose tissues |
title_fullStr |
Differentiation and traffic of IgM+ B cells between focal dark spots in skeletal muscle of Atlantic salmon, lymphoid and adipose tissues |
title_full_unstemmed |
Differentiation and traffic of IgM+ B cells between focal dark spots in skeletal muscle of Atlantic salmon, lymphoid and adipose tissues |
title_sort |
differentiation and traffic of igm+ b cells between focal dark spots in skeletal muscle of atlantic salmon, lymphoid and adipose tissues |
publisher |
HAL CCSD |
publishDate |
2023 |
url |
https://hal.science/hal-04190866 https://hal.science/hal-04190866/document https://hal.science/hal-04190866/file/1-s2.0-S1050464823003443-main.pdf https://doi.org/10.1016/j.fsi.2023.108858 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
ISSN: 1050-4648 Fish & Shellfish Immunology https://hal.science/hal-04190866 Fish & Shellfish Immunology, 2023, 139, pp.108858. ⟨10.1016/j.fsi.2023.108858⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.fsi.2023.108858 info:eu-repo/semantics/altIdentifier/pmid/37302676 hal-04190866 https://hal.science/hal-04190866 https://hal.science/hal-04190866/document https://hal.science/hal-04190866/file/1-s2.0-S1050464823003443-main.pdf doi:10.1016/j.fsi.2023.108858 PUBMED: 37302676 WOS: 001027524500001 |
op_rights |
http://creativecommons.org/licenses/by/ info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1016/j.fsi.2023.108858 |
container_title |
Fish & Shellfish Immunology |
container_volume |
139 |
container_start_page |
108858 |
_version_ |
1810432417989656576 |
spelling |
ftanrparis:oai:HAL:hal-04190866v1 2024-09-15T17:56:12+00:00 Differentiation and traffic of IgM+ B cells between focal dark spots in skeletal muscle of Atlantic salmon, lymphoid and adipose tissues Jiménez-Guerrero, Raúl Karlsen, Christian René Boudinot, Pierre Afanasyev, Sergey Mørkøre, Turid Krasnov, Aleksei Virologie et Immunologie Moléculaires (VIM (UR 0892)) Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) Norwegian Directorate of Fisheries for large-scale Industrial Research; Sechenov Institute of Evolutionary Physiology and Biochemistry of RAS, (075-00967-23-00); Agence Nationale de la Recherche, ANR, (ANR-21-CE35-0019); Norges Miljø- og Biovitenskapelige Universitet, NMBU; Fiskeri - og havbruksnæringens forskningsfond, FHF, (901487) ANR-21-CE35-0019,LipoFishVac,Vaccins liponanoparticulaires mARN et ADN pour les poissons, couplés à des adjuvants immunomodulateurs(2021) 2023 https://hal.science/hal-04190866 https://hal.science/hal-04190866/document https://hal.science/hal-04190866/file/1-s2.0-S1050464823003443-main.pdf https://doi.org/10.1016/j.fsi.2023.108858 en eng HAL CCSD info:eu-repo/semantics/altIdentifier/doi/10.1016/j.fsi.2023.108858 info:eu-repo/semantics/altIdentifier/pmid/37302676 hal-04190866 https://hal.science/hal-04190866 https://hal.science/hal-04190866/document https://hal.science/hal-04190866/file/1-s2.0-S1050464823003443-main.pdf doi:10.1016/j.fsi.2023.108858 PUBMED: 37302676 WOS: 001027524500001 http://creativecommons.org/licenses/by/ info:eu-repo/semantics/OpenAccess ISSN: 1050-4648 Fish & Shellfish Immunology https://hal.science/hal-04190866 Fish & Shellfish Immunology, 2023, 139, pp.108858. ⟨10.1016/j.fsi.2023.108858⟩ Atlantic salmon B-cell Gene expression Healing IgM repertoire Melanin spots [SDV]Life Sciences [q-bio] info:eu-repo/semantics/article Journal articles 2023 ftanrparis https://doi.org/10.1016/j.fsi.2023.108858 2024-07-12T10:49:00Z International audience Focal dark spots (DS) in farmed Atlantic salmon fillets contain a significant number of B cells as revealed by the high abundance of immunoglobulin (Ig) transcripts in transcriptome data. The immune response in DS remains unknown while they represent a major problem in commercial aquaculture. Here, we characterized the diversity and clonal composition of B cells in DS. Sixteen gene markers of immune cells and antigen presentation were analyzed with RT-qPCR. All genes expression showed a positive correlation with DS area and intensity. The flatter the DS, the higher the expression of cd28, csfr, ctla, igt, and sigm, the lower expression of cd83 and btla, and the larger the cumulative frequency within DS. The expression of most of the analyzed immune genes, including three Ig types and markers of B cells was lower in DS than in the lymphatic organs, head kidney and spleen, but significantly higher compared to skeletal muscle. High levels of ctla4 and cd28 in DS might indicate the recruitment of T cells. Sequencing of IgM repertoire (Ig-seq) assessed migration of B cells by co-occurrence of identical CDR3 sequences in different tissues. The combination of gene expression and Ig-seq revealed the presence of several stages of B cell differentiation in DS. B cells at the earliest stage, with high ratio of membrane to secretory IgM (migm and sigm), showed minor Ig repertoire overlap with other tissues. Further differentiation stage (increased sigm to migm ratio and high expression of pax5 and cd79) was associated with active movement of B cells from DS towards lymphatic organs and visceral fat. Traffic and expression of immune genes decreased at later stages. These B cells could be involved in a response directed against viruses, pathogenic or opportunistic bacteria in DS. Seven of eight fish were positive for salmon alphavirus, and levels were higher in DS than in unstained muscle. PCR with universal primers to the 16S rRNA gene did not detect bacteria in DS. Although the evolution of DS most ... Article in Journal/Newspaper Atlantic salmon Portail HAL-ANR (Agence Nationale de la Recherche) Fish & Shellfish Immunology 139 108858 |