Molecular and phenotypic effects of early exposure to an environmentally relevant pesticide mixture in the Pacific oyster, Crassostrea gigas.
International audience Early life stages are crucial for organism development, especially for those displaying external fertilization, whose gametes and early stages face environmental stressors such as xenobiotics. The pacific oyster, Crassostrea gigas, is considered a model species in ecotoxicolog...
Published in: | Environmental Pollution |
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Main Authors: | , , , , , , , , , |
Other Authors: | , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
HAL CCSD
2023
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Subjects: | |
Online Access: | https://hal.univ-brest.fr/hal-04101505 https://doi.org/10.1016/j.envpol.2023.121472 |
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ftanrparis:oai:HAL:hal-04101505v1 |
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openpolar |
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Open Polar |
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Portail HAL-ANR (Agence Nationale de la Recherche) |
op_collection_id |
ftanrparis |
language |
English |
topic |
Mixture Epigenetics Gene expression Molluscs Pesticides DNA methylation [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology |
spellingShingle |
Mixture Epigenetics Gene expression Molluscs Pesticides DNA methylation [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology Sol Dourdin, Thomas Rivière, Guillaume Cormier, Alexandre Di Poi, Carole Guyomard, Killian Rabiller, Manuella Akcha, Farida Bah Thierno, Sadialiou Le Monier, Pauline Sussarellu, Rossana Molecular and phenotypic effects of early exposure to an environmentally relevant pesticide mixture in the Pacific oyster, Crassostrea gigas. |
topic_facet |
Mixture Epigenetics Gene expression Molluscs Pesticides DNA methylation [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology |
description |
International audience Early life stages are crucial for organism development, especially for those displaying external fertilization, whose gametes and early stages face environmental stressors such as xenobiotics. The pacific oyster, Crassostrea gigas, is considered a model species in ecotoxicology because of its ecological characteristics (benthic, sessile, filter feeding). So far studies have investigated the impact of xenobiotics at embryotoxic, genotoxic and physi-ological endpoints, sometimes at the multigenerational scale, highlighting the role of epigenetic mechanisms in transmitting alterations induced by exposure to single xenobiotics. However, to date, little is known about the impact of environmentally-mimicking contaminants cocktails. Thus, we examined the impact of an early exposure to environmentally relevant mixture on the Pacific oyster life history. We studied transcriptomic, epigenetic and physiological alterations induced in oysters exposed to 18 pesticides and metals at environmental concentration (nominal sum concentration: 2.85 mu g.L-1, measured sum concentration: 3.74 +/- 0.013 mu g.L-1) during embryo-larval stage (0-48 h post fertilization, hpf). No significant differences in embryo-larval abnor-malities at 24 hpf were observed during larval and spat rearing; the swimming behaviour of exposed individuals was disturbed, while they were longer and heavier at specific time points, and exhibited a lower epinephrine-induced metamorphosis rate as well as a higher survival rate in the field. In addition, RNA-seq analyses of gastrula embryos revealed the differential expression of development-related genes (e.g. Hox orthologues and cell cycle regulators) between control and exposed oysters. Whole-genome DNA methylation analyses demon-strated a significant modification of DNA methylation in exposed larvae marked by a demethylation trend. Those findings suggest that early exposure to an environmentally relevant pesticide mixture induces multi-scale latent effects possibly affecting life ... |
author2 |
Unité Contamination Chimique des Ecosystèmes Marins (CCEM) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Biologie des Organismes et Ecosystèmes Aquatiques (BOREA) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA) Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Plateforme expérimentale Mollusques Marins de Bouin (EMMA-PMMB) Unité Expérimentale Mollusques Marins Atlantique (EMMA) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) ANR-19-CE34-0004,PESTO,Effets développementaux et multigénérationnels suite à une exposition à des pesticides chez l'huître(2019) |
format |
Article in Journal/Newspaper |
author |
Sol Dourdin, Thomas Rivière, Guillaume Cormier, Alexandre Di Poi, Carole Guyomard, Killian Rabiller, Manuella Akcha, Farida Bah Thierno, Sadialiou Le Monier, Pauline Sussarellu, Rossana |
author_facet |
Sol Dourdin, Thomas Rivière, Guillaume Cormier, Alexandre Di Poi, Carole Guyomard, Killian Rabiller, Manuella Akcha, Farida Bah Thierno, Sadialiou Le Monier, Pauline Sussarellu, Rossana |
author_sort |
Sol Dourdin, Thomas |
title |
Molecular and phenotypic effects of early exposure to an environmentally relevant pesticide mixture in the Pacific oyster, Crassostrea gigas. |
title_short |
Molecular and phenotypic effects of early exposure to an environmentally relevant pesticide mixture in the Pacific oyster, Crassostrea gigas. |
title_full |
Molecular and phenotypic effects of early exposure to an environmentally relevant pesticide mixture in the Pacific oyster, Crassostrea gigas. |
title_fullStr |
Molecular and phenotypic effects of early exposure to an environmentally relevant pesticide mixture in the Pacific oyster, Crassostrea gigas. |
title_full_unstemmed |
Molecular and phenotypic effects of early exposure to an environmentally relevant pesticide mixture in the Pacific oyster, Crassostrea gigas. |
title_sort |
molecular and phenotypic effects of early exposure to an environmentally relevant pesticide mixture in the pacific oyster, crassostrea gigas. |
publisher |
HAL CCSD |
publishDate |
2023 |
url |
https://hal.univ-brest.fr/hal-04101505 https://doi.org/10.1016/j.envpol.2023.121472 |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 0013-9327 Environmental Pollution (1970) https://hal.univ-brest.fr/hal-04101505 Environmental Pollution (1970), 2023, 326, pp.121472. ⟨10.1016/j.envpol.2023.121472⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.envpol.2023.121472 hal-04101505 https://hal.univ-brest.fr/hal-04101505 doi:10.1016/j.envpol.2023.121472 |
op_doi |
https://doi.org/10.1016/j.envpol.2023.121472 |
container_title |
Environmental Pollution |
container_volume |
326 |
container_start_page |
121472 |
_version_ |
1810440708976279552 |
spelling |
ftanrparis:oai:HAL:hal-04101505v1 2024-09-15T18:03:12+00:00 Molecular and phenotypic effects of early exposure to an environmentally relevant pesticide mixture in the Pacific oyster, Crassostrea gigas. Sol Dourdin, Thomas Rivière, Guillaume Cormier, Alexandre Di Poi, Carole Guyomard, Killian Rabiller, Manuella Akcha, Farida Bah Thierno, Sadialiou Le Monier, Pauline Sussarellu, Rossana Unité Contamination Chimique des Ecosystèmes Marins (CCEM) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Biologie des Organismes et Ecosystèmes Aquatiques (BOREA) Université de Caen Normandie (UNICAEN) Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA) Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Plateforme expérimentale Mollusques Marins de Bouin (EMMA-PMMB) Unité Expérimentale Mollusques Marins Atlantique (EMMA) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) ANR-19-CE34-0004,PESTO,Effets développementaux et multigénérationnels suite à une exposition à des pesticides chez l'huître(2019) 2023-06-01 https://hal.univ-brest.fr/hal-04101505 https://doi.org/10.1016/j.envpol.2023.121472 en eng HAL CCSD Elsevier Science info:eu-repo/semantics/altIdentifier/doi/10.1016/j.envpol.2023.121472 hal-04101505 https://hal.univ-brest.fr/hal-04101505 doi:10.1016/j.envpol.2023.121472 ISSN: 0013-9327 Environmental Pollution (1970) https://hal.univ-brest.fr/hal-04101505 Environmental Pollution (1970), 2023, 326, pp.121472. ⟨10.1016/j.envpol.2023.121472⟩ Mixture Epigenetics Gene expression Molluscs Pesticides DNA methylation [SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology info:eu-repo/semantics/article Journal articles 2023 ftanrparis https://doi.org/10.1016/j.envpol.2023.121472 2024-07-12T10:51:37Z International audience Early life stages are crucial for organism development, especially for those displaying external fertilization, whose gametes and early stages face environmental stressors such as xenobiotics. The pacific oyster, Crassostrea gigas, is considered a model species in ecotoxicology because of its ecological characteristics (benthic, sessile, filter feeding). So far studies have investigated the impact of xenobiotics at embryotoxic, genotoxic and physi-ological endpoints, sometimes at the multigenerational scale, highlighting the role of epigenetic mechanisms in transmitting alterations induced by exposure to single xenobiotics. However, to date, little is known about the impact of environmentally-mimicking contaminants cocktails. Thus, we examined the impact of an early exposure to environmentally relevant mixture on the Pacific oyster life history. We studied transcriptomic, epigenetic and physiological alterations induced in oysters exposed to 18 pesticides and metals at environmental concentration (nominal sum concentration: 2.85 mu g.L-1, measured sum concentration: 3.74 +/- 0.013 mu g.L-1) during embryo-larval stage (0-48 h post fertilization, hpf). No significant differences in embryo-larval abnor-malities at 24 hpf were observed during larval and spat rearing; the swimming behaviour of exposed individuals was disturbed, while they were longer and heavier at specific time points, and exhibited a lower epinephrine-induced metamorphosis rate as well as a higher survival rate in the field. In addition, RNA-seq analyses of gastrula embryos revealed the differential expression of development-related genes (e.g. Hox orthologues and cell cycle regulators) between control and exposed oysters. Whole-genome DNA methylation analyses demon-strated a significant modification of DNA methylation in exposed larvae marked by a demethylation trend. Those findings suggest that early exposure to an environmentally relevant pesticide mixture induces multi-scale latent effects possibly affecting life ... Article in Journal/Newspaper Crassostrea gigas Pacific oyster Portail HAL-ANR (Agence Nationale de la Recherche) Environmental Pollution 326 121472 |