Prevalence and Molecular Characterization of Rickettsia spp. from Wild Small Mammals in Public Parks and Urban Areas of Bangkok Metropolitan, Thailand
International audience Rural areas usually show a higher prevalence of rickettsial infection than urban areas. However, information on the rickettsial infection status in urban settings (e.g., built-up areas and city parks) is still limited, particularly in the Bangkok metropolitan area. In this stu...
Published in: | Tropical Medicine and Infectious Disease |
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ftanrparis:oai:HAL:hal-03535109v1 2024-09-15T18:32:02+00:00 Prevalence and Molecular Characterization of Rickettsia spp. from Wild Small Mammals in Public Parks and Urban Areas of Bangkok Metropolitan, Thailand Rungrojn, Artharee Chaisiri, Kittipong Paladsing, Yossapong Morand, Serge Junjhon, Jiraphan Blacksell, Stuart Ekchariyawat, Peeraya Mahidol University Bangkok Department of Clinical Tropical Medicine Bangkok, Thailand (Faculty of Tropical Medicine) Animal, Santé, Territoires, Risques et Ecosystèmes (UMR ASTRE) Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) Faculty of Veterinary Medicine Kasetsart University, Thaïlande Kasetsart University Bangkok, Thailand (KU)-Partenaires IRSTEA Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA)-Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA) Mahidol Oxford Tropical Medicine Research Unit (MORU) University of Oxford-Mahidol University Bangkok -Wellcome Trust Nuffield Department of Clinical Medicine Oxford University of Oxford Stuart D. Blacksell was funded by the Wellcome Trust of the United Kingdom. This research was funded in whole, or in part, by the Wellcome Trust (220211). ANR-17-CE35-0003,FutureHealthSEA,Scénarios de la santé en Asie du Sud-Est: changements d'utilisation des terres, changement climatique et maladies infectieuses(2017) 2021-12 https://hal.inrae.fr/hal-03535109 https://hal.inrae.fr/hal-03535109/document https://hal.inrae.fr/hal-03535109/file/tropicalmed-06-00199.pdf https://doi.org/10.3390/tropicalmed6040199 en eng HAL CCSD MDPI info:eu-repo/semantics/altIdentifier/doi/10.3390/tropicalmed6040199 info:eu-repo/semantics/altIdentifier/pmid/34842856 hal-03535109 https://hal.inrae.fr/hal-03535109 https://hal.inrae.fr/hal-03535109/document https://hal.inrae.fr/hal-03535109/file/tropicalmed-06-00199.pdf doi:10.3390/tropicalmed6040199 PUBMED: 34842856 WOS: 000738388600001 http://creativecommons.org/licenses/by/ info:eu-repo/semantics/OpenAccess ISSN: 2414-6366 Tropical Medicine and Infectious Disease https://hal.inrae.fr/hal-03535109 Tropical Medicine and Infectious Disease, 2021, 6 (4), pp.199. ⟨10.3390/tropicalmed6040199⟩ Rickettsia typhi Rickettsia felis small mammals rodents public parks urban Bangkok [SDV]Life Sciences [q-bio] info:eu-repo/semantics/article Journal articles 2021 ftanrparis https://doi.org/10.3390/tropicalmed6040199 2024-07-12T11:02:45Z International audience Rural areas usually show a higher prevalence of rickettsial infection than urban areas. However, information on the rickettsial infection status in urban settings (e.g., built-up areas and city parks) is still limited, particularly in the Bangkok metropolitan area. In this study, we performed a molecular rickettsial survey of spleen samples of small mammals caught in public parks and built-up areas of Bangkok. Out of 198 samples, the Rattus rattus complex was found to be most prevalent. The amplification of rickettsial gltA fragment gene (338 bp) by nested PCR assay revealed positive results in four samples, yielding a low prevalence of infection of 2.02%. DNA sequencing results confirmed that three samples were matched with Rickettsia typhi, and one was identified as R. felis. It is noteworthy that this is the first report of the occurrence of R. felis DNA in rodents in Southeast Asia. Article in Journal/Newspaper Rattus rattus Portail HAL-ANR (Agence Nationale de la Recherche) Tropical Medicine and Infectious Disease 6 4 199 |
institution |
Open Polar |
collection |
Portail HAL-ANR (Agence Nationale de la Recherche) |
op_collection_id |
ftanrparis |
language |
English |
topic |
Rickettsia typhi Rickettsia felis small mammals rodents public parks urban Bangkok [SDV]Life Sciences [q-bio] |
spellingShingle |
Rickettsia typhi Rickettsia felis small mammals rodents public parks urban Bangkok [SDV]Life Sciences [q-bio] Rungrojn, Artharee Chaisiri, Kittipong Paladsing, Yossapong Morand, Serge Junjhon, Jiraphan Blacksell, Stuart Ekchariyawat, Peeraya Prevalence and Molecular Characterization of Rickettsia spp. from Wild Small Mammals in Public Parks and Urban Areas of Bangkok Metropolitan, Thailand |
topic_facet |
Rickettsia typhi Rickettsia felis small mammals rodents public parks urban Bangkok [SDV]Life Sciences [q-bio] |
description |
International audience Rural areas usually show a higher prevalence of rickettsial infection than urban areas. However, information on the rickettsial infection status in urban settings (e.g., built-up areas and city parks) is still limited, particularly in the Bangkok metropolitan area. In this study, we performed a molecular rickettsial survey of spleen samples of small mammals caught in public parks and built-up areas of Bangkok. Out of 198 samples, the Rattus rattus complex was found to be most prevalent. The amplification of rickettsial gltA fragment gene (338 bp) by nested PCR assay revealed positive results in four samples, yielding a low prevalence of infection of 2.02%. DNA sequencing results confirmed that three samples were matched with Rickettsia typhi, and one was identified as R. felis. It is noteworthy that this is the first report of the occurrence of R. felis DNA in rodents in Southeast Asia. |
author2 |
Mahidol University Bangkok Department of Clinical Tropical Medicine Bangkok, Thailand (Faculty of Tropical Medicine) Animal, Santé, Territoires, Risques et Ecosystèmes (UMR ASTRE) Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) Faculty of Veterinary Medicine Kasetsart University, Thaïlande Kasetsart University Bangkok, Thailand (KU)-Partenaires IRSTEA Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA)-Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA) Mahidol Oxford Tropical Medicine Research Unit (MORU) University of Oxford-Mahidol University Bangkok -Wellcome Trust Nuffield Department of Clinical Medicine Oxford University of Oxford Stuart D. Blacksell was funded by the Wellcome Trust of the United Kingdom. This research was funded in whole, or in part, by the Wellcome Trust (220211). ANR-17-CE35-0003,FutureHealthSEA,Scénarios de la santé en Asie du Sud-Est: changements d'utilisation des terres, changement climatique et maladies infectieuses(2017) |
format |
Article in Journal/Newspaper |
author |
Rungrojn, Artharee Chaisiri, Kittipong Paladsing, Yossapong Morand, Serge Junjhon, Jiraphan Blacksell, Stuart Ekchariyawat, Peeraya |
author_facet |
Rungrojn, Artharee Chaisiri, Kittipong Paladsing, Yossapong Morand, Serge Junjhon, Jiraphan Blacksell, Stuart Ekchariyawat, Peeraya |
author_sort |
Rungrojn, Artharee |
title |
Prevalence and Molecular Characterization of Rickettsia spp. from Wild Small Mammals in Public Parks and Urban Areas of Bangkok Metropolitan, Thailand |
title_short |
Prevalence and Molecular Characterization of Rickettsia spp. from Wild Small Mammals in Public Parks and Urban Areas of Bangkok Metropolitan, Thailand |
title_full |
Prevalence and Molecular Characterization of Rickettsia spp. from Wild Small Mammals in Public Parks and Urban Areas of Bangkok Metropolitan, Thailand |
title_fullStr |
Prevalence and Molecular Characterization of Rickettsia spp. from Wild Small Mammals in Public Parks and Urban Areas of Bangkok Metropolitan, Thailand |
title_full_unstemmed |
Prevalence and Molecular Characterization of Rickettsia spp. from Wild Small Mammals in Public Parks and Urban Areas of Bangkok Metropolitan, Thailand |
title_sort |
prevalence and molecular characterization of rickettsia spp. from wild small mammals in public parks and urban areas of bangkok metropolitan, thailand |
publisher |
HAL CCSD |
publishDate |
2021 |
url |
https://hal.inrae.fr/hal-03535109 https://hal.inrae.fr/hal-03535109/document https://hal.inrae.fr/hal-03535109/file/tropicalmed-06-00199.pdf https://doi.org/10.3390/tropicalmed6040199 |
genre |
Rattus rattus |
genre_facet |
Rattus rattus |
op_source |
ISSN: 2414-6366 Tropical Medicine and Infectious Disease https://hal.inrae.fr/hal-03535109 Tropical Medicine and Infectious Disease, 2021, 6 (4), pp.199. ⟨10.3390/tropicalmed6040199⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.3390/tropicalmed6040199 info:eu-repo/semantics/altIdentifier/pmid/34842856 hal-03535109 https://hal.inrae.fr/hal-03535109 https://hal.inrae.fr/hal-03535109/document https://hal.inrae.fr/hal-03535109/file/tropicalmed-06-00199.pdf doi:10.3390/tropicalmed6040199 PUBMED: 34842856 WOS: 000738388600001 |
op_rights |
http://creativecommons.org/licenses/by/ info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.3390/tropicalmed6040199 |
container_title |
Tropical Medicine and Infectious Disease |
container_volume |
6 |
container_issue |
4 |
container_start_page |
199 |
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1810473779692830720 |