Genetic Potential of Dissulfurimicrobium hydrothermale, an Obligate Sulfur-Disproportionating Thermophilic Microorganism
International audience The biochemical pathways of anaerobic sulfur disproportionation are only partially deciphered, and the mechanisms involved in the first step of S0-disproportionation remain unknown. Here, we present the results of sequencing and analysis of the complete genome of Dissulfurimic...
Published in: | Microorganisms |
---|---|
Main Authors: | , , , , , |
Other Authors: | , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
HAL CCSD
2021
|
Subjects: | |
Online Access: | https://hal.science/hal-03504518 https://hal.science/hal-03504518/document https://hal.science/hal-03504518/file/Yvenou_2021_Dissulfurimicrobium_hydrothermale.pdf https://doi.org/10.3390/microorganisms10010060 |
id |
ftanrparis:oai:HAL:hal-03504518v1 |
---|---|
record_format |
openpolar |
spelling |
ftanrparis:oai:HAL:hal-03504518v1 2024-05-12T08:06:20+00:00 Genetic Potential of Dissulfurimicrobium hydrothermale, an Obligate Sulfur-Disproportionating Thermophilic Microorganism Yvenou, Stéven Allioux, Maxime Slobodkin, Alexander Slobodkina, Galina Jebbar, Mohamed Alain, Karine Laboratoire de Microbiologie des Environnements Extrêmophiles (LM2E) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS) ANR-17-EURE-0015,ISBlue,Interdisciplinary Graduate School for the Blue planet(2017) 2021-12-28 https://hal.science/hal-03504518 https://hal.science/hal-03504518/document https://hal.science/hal-03504518/file/Yvenou_2021_Dissulfurimicrobium_hydrothermale.pdf https://doi.org/10.3390/microorganisms10010060 en eng HAL CCSD MDPI info:eu-repo/semantics/altIdentifier/doi/10.3390/microorganisms10010060 hal-03504518 https://hal.science/hal-03504518 https://hal.science/hal-03504518/document https://hal.science/hal-03504518/file/Yvenou_2021_Dissulfurimicrobium_hydrothermale.pdf doi:10.3390/microorganisms10010060 info:eu-repo/semantics/OpenAccess ISSN: 2076-2607 Microorganisms https://hal.science/hal-03504518 Microorganisms, 2021, 10, ⟨10.3390/microorganisms10010060⟩ G Sulfur-Disproportionating hydrothermal vent genomics thermophile [SDE.BE]Environmental Sciences/Biodiversity and Ecology info:eu-repo/semantics/article Journal articles 2021 ftanrparis https://doi.org/10.3390/microorganisms10010060 2024-04-16T03:07:10Z International audience The biochemical pathways of anaerobic sulfur disproportionation are only partially deciphered, and the mechanisms involved in the first step of S0-disproportionation remain unknown. Here, we present the results of sequencing and analysis of the complete genome of Dissulfurimicrobium hydrothermale strain Sh68T, one of two strains isolated to date known to grow exclusively by anaerobic disproportionation of inorganic sulfur compounds. Dissulfurimicrobium hydrothermale Sh68T is a motile, thermophilic, anaerobic, chemolithoautotrophic microorganism isolated from a hydrothermal pond at Uzon caldera, Kamchatka, Russia. It is able to produce energy and grow by disproportionation of elemental sulfur, sulfite and thiosulfate. Its genome consists of a circular chromosome of 2,025,450 base pairs, has a G + C content of 49.66% and a completion of 97.6%. Genomic data suggest that CO2 assimilation is carried out by the Wood–Ljungdhal pathway and that central anabolism involves the gluconeogenesis pathway. The genome of strain Sh68T encodes the complete gene set of the dissimilatory sulfate reduction pathway, some of which are likely to be involved in sulfur disproportionation. A short sequence protein of unknown function present in the genome of strain Sh68T is conserved in the genomes of a large panel of other S0-disproportionating bacteria and was absent from the genomes of microorganisms incapable of elemental sulfur disproportionation. We propose that this protein may be involved in the first step of elemental sulfur disproportionation, as S0 is poorly soluble and unable to cross the cytoplasmic membrane in this form Article in Journal/Newspaper Kamchatka Portail HAL-ANR (Agence Nationale de la Recherche) Microorganisms 10 1 60 |
institution |
Open Polar |
collection |
Portail HAL-ANR (Agence Nationale de la Recherche) |
op_collection_id |
ftanrparis |
language |
English |
topic |
G Sulfur-Disproportionating hydrothermal vent genomics thermophile [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
spellingShingle |
G Sulfur-Disproportionating hydrothermal vent genomics thermophile [SDE.BE]Environmental Sciences/Biodiversity and Ecology Yvenou, Stéven Allioux, Maxime Slobodkin, Alexander Slobodkina, Galina Jebbar, Mohamed Alain, Karine Genetic Potential of Dissulfurimicrobium hydrothermale, an Obligate Sulfur-Disproportionating Thermophilic Microorganism |
topic_facet |
G Sulfur-Disproportionating hydrothermal vent genomics thermophile [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
description |
International audience The biochemical pathways of anaerobic sulfur disproportionation are only partially deciphered, and the mechanisms involved in the first step of S0-disproportionation remain unknown. Here, we present the results of sequencing and analysis of the complete genome of Dissulfurimicrobium hydrothermale strain Sh68T, one of two strains isolated to date known to grow exclusively by anaerobic disproportionation of inorganic sulfur compounds. Dissulfurimicrobium hydrothermale Sh68T is a motile, thermophilic, anaerobic, chemolithoautotrophic microorganism isolated from a hydrothermal pond at Uzon caldera, Kamchatka, Russia. It is able to produce energy and grow by disproportionation of elemental sulfur, sulfite and thiosulfate. Its genome consists of a circular chromosome of 2,025,450 base pairs, has a G + C content of 49.66% and a completion of 97.6%. Genomic data suggest that CO2 assimilation is carried out by the Wood–Ljungdhal pathway and that central anabolism involves the gluconeogenesis pathway. The genome of strain Sh68T encodes the complete gene set of the dissimilatory sulfate reduction pathway, some of which are likely to be involved in sulfur disproportionation. A short sequence protein of unknown function present in the genome of strain Sh68T is conserved in the genomes of a large panel of other S0-disproportionating bacteria and was absent from the genomes of microorganisms incapable of elemental sulfur disproportionation. We propose that this protein may be involved in the first step of elemental sulfur disproportionation, as S0 is poorly soluble and unable to cross the cytoplasmic membrane in this form |
author2 |
Laboratoire de Microbiologie des Environnements Extrêmophiles (LM2E) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS) ANR-17-EURE-0015,ISBlue,Interdisciplinary Graduate School for the Blue planet(2017) |
format |
Article in Journal/Newspaper |
author |
Yvenou, Stéven Allioux, Maxime Slobodkin, Alexander Slobodkina, Galina Jebbar, Mohamed Alain, Karine |
author_facet |
Yvenou, Stéven Allioux, Maxime Slobodkin, Alexander Slobodkina, Galina Jebbar, Mohamed Alain, Karine |
author_sort |
Yvenou, Stéven |
title |
Genetic Potential of Dissulfurimicrobium hydrothermale, an Obligate Sulfur-Disproportionating Thermophilic Microorganism |
title_short |
Genetic Potential of Dissulfurimicrobium hydrothermale, an Obligate Sulfur-Disproportionating Thermophilic Microorganism |
title_full |
Genetic Potential of Dissulfurimicrobium hydrothermale, an Obligate Sulfur-Disproportionating Thermophilic Microorganism |
title_fullStr |
Genetic Potential of Dissulfurimicrobium hydrothermale, an Obligate Sulfur-Disproportionating Thermophilic Microorganism |
title_full_unstemmed |
Genetic Potential of Dissulfurimicrobium hydrothermale, an Obligate Sulfur-Disproportionating Thermophilic Microorganism |
title_sort |
genetic potential of dissulfurimicrobium hydrothermale, an obligate sulfur-disproportionating thermophilic microorganism |
publisher |
HAL CCSD |
publishDate |
2021 |
url |
https://hal.science/hal-03504518 https://hal.science/hal-03504518/document https://hal.science/hal-03504518/file/Yvenou_2021_Dissulfurimicrobium_hydrothermale.pdf https://doi.org/10.3390/microorganisms10010060 |
genre |
Kamchatka |
genre_facet |
Kamchatka |
op_source |
ISSN: 2076-2607 Microorganisms https://hal.science/hal-03504518 Microorganisms, 2021, 10, ⟨10.3390/microorganisms10010060⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.3390/microorganisms10010060 hal-03504518 https://hal.science/hal-03504518 https://hal.science/hal-03504518/document https://hal.science/hal-03504518/file/Yvenou_2021_Dissulfurimicrobium_hydrothermale.pdf doi:10.3390/microorganisms10010060 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.3390/microorganisms10010060 |
container_title |
Microorganisms |
container_volume |
10 |
container_issue |
1 |
container_start_page |
60 |
_version_ |
1798848817032855552 |