Long-lasting antiviral innate immune priming in the Lophotrochozoan Pacific oyster, Crassostrea gigas
International audience In the last decade, a paradigm shift has emerged in comparative immunology. Invertebrates can no longer be considered to be devoid of specific recognition and immune memory. However, we still lack a comprehensive view of these phenomena and their molecular mechanisms across ph...
Published in: | Scientific Reports |
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Main Authors: | , , , , , , |
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Format: | Article in Journal/Newspaper |
Language: | English |
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HAL CCSD
2017
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Online Access: | https://hal.science/hal-01632346 https://hal.science/hal-01632346/document https://hal.science/hal-01632346/file/Lafont-2017-SciRep.LongLasting.pdf https://doi.org/10.1038/s41598-017-13564-0 |
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openpolar |
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Open Polar |
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Portail HAL-ANR (Agence Nationale de la Recherche) |
op_collection_id |
ftanrparis |
language |
English |
topic |
MEMORY INFECTION IN-VIVO INVERTEBRATE IMMUNITY DOUBLE-STRANDED-RNA SPOT SYNDROME VIRUS OSTREID HERPESVIRUS-1 SECONDARY CHALLENGE VIBRIO-SPLENDIDUS RESPONSES ACL [SDV.IMM.II]Life Sciences [q-bio]/Immunology/Innate immunity [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
spellingShingle |
MEMORY INFECTION IN-VIVO INVERTEBRATE IMMUNITY DOUBLE-STRANDED-RNA SPOT SYNDROME VIRUS OSTREID HERPESVIRUS-1 SECONDARY CHALLENGE VIBRIO-SPLENDIDUS RESPONSES ACL [SDV.IMM.II]Life Sciences [q-bio]/Immunology/Innate immunity [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology [SDE.BE]Environmental Sciences/Biodiversity and Ecology Lafont, Maxime Petton, Bruno Vergnes, Agnès Pauletto, Marianna Segarra, Amélie Gourbal, Benjamin Montagnani, Caroline Long-lasting antiviral innate immune priming in the Lophotrochozoan Pacific oyster, Crassostrea gigas |
topic_facet |
MEMORY INFECTION IN-VIVO INVERTEBRATE IMMUNITY DOUBLE-STRANDED-RNA SPOT SYNDROME VIRUS OSTREID HERPESVIRUS-1 SECONDARY CHALLENGE VIBRIO-SPLENDIDUS RESPONSES ACL [SDV.IMM.II]Life Sciences [q-bio]/Immunology/Innate immunity [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
description |
International audience In the last decade, a paradigm shift has emerged in comparative immunology. Invertebrates can no longer be considered to be devoid of specific recognition and immune memory. However, we still lack a comprehensive view of these phenomena and their molecular mechanisms across phyla, especially in terms of duration, specificity, and efficiency in a natural context. In this study, we focused on a Lophotrochozoan/virus interaction, as antiviral priming is mostly overlooked in molluscs. Juvenile Crassostrea gigas oysters experience reoccurring mass mortalities events from Ostreid herpes virus 1 with no existing therapeutic treatment. Our results showed that various nucleic acid injections can prime oysters to trigger an antiviral state ultimately protecting them against a subsequent viral infection. Focusing on poly(I:C) as elicitor, we evidenced that it protected from an environmental infection, by mitigating viral replication. That protection seemed to induce a specific antiviral response as poly(I:C) fails to protect against a pathogenic bacteria. Finally, we showed that this phenomenon was long-lasting, persisting for at least 5 months thus suggesting for the first time the existence of innate immune memory in this invertebrate species. This study strengthens the emerging hypotheses about the broad conservation of innate immune priming and memory mechanisms in Lophotrochozoans. |
author2 |
Interactions Hôtes-Pathogènes-Environnements (IHPE) Université de Perpignan Via Domitia (UPVD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM) Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Università degli Studi di Padova = University of Padua (Unipd) ANR-14-CE19-0023,DECIPHER,Déchiffrage des maladies multifactorielles: cas des mortalités de l'huître(2014) |
format |
Article in Journal/Newspaper |
author |
Lafont, Maxime Petton, Bruno Vergnes, Agnès Pauletto, Marianna Segarra, Amélie Gourbal, Benjamin Montagnani, Caroline |
author_facet |
Lafont, Maxime Petton, Bruno Vergnes, Agnès Pauletto, Marianna Segarra, Amélie Gourbal, Benjamin Montagnani, Caroline |
author_sort |
Lafont, Maxime |
title |
Long-lasting antiviral innate immune priming in the Lophotrochozoan Pacific oyster, Crassostrea gigas |
title_short |
Long-lasting antiviral innate immune priming in the Lophotrochozoan Pacific oyster, Crassostrea gigas |
title_full |
Long-lasting antiviral innate immune priming in the Lophotrochozoan Pacific oyster, Crassostrea gigas |
title_fullStr |
Long-lasting antiviral innate immune priming in the Lophotrochozoan Pacific oyster, Crassostrea gigas |
title_full_unstemmed |
Long-lasting antiviral innate immune priming in the Lophotrochozoan Pacific oyster, Crassostrea gigas |
title_sort |
long-lasting antiviral innate immune priming in the lophotrochozoan pacific oyster, crassostrea gigas |
publisher |
HAL CCSD |
publishDate |
2017 |
url |
https://hal.science/hal-01632346 https://hal.science/hal-01632346/document https://hal.science/hal-01632346/file/Lafont-2017-SciRep.LongLasting.pdf https://doi.org/10.1038/s41598-017-13564-0 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
ISSN: 2045-2322 EISSN: 2045-2322 Scientific Reports https://hal.science/hal-01632346 Scientific Reports, 2017, 7, pp.13143. ⟨10.1038/s41598-017-13564-0⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-017-13564-0 hal-01632346 https://hal.science/hal-01632346 https://hal.science/hal-01632346/document https://hal.science/hal-01632346/file/Lafont-2017-SciRep.LongLasting.pdf doi:10.1038/s41598-017-13564-0 |
op_rights |
info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1038/s41598-017-13564-0 |
container_title |
Scientific Reports |
container_volume |
7 |
container_issue |
1 |
_version_ |
1785581408270417920 |
spelling |
ftanrparis:oai:HAL:hal-01632346v1 2023-12-17T10:29:07+01:00 Long-lasting antiviral innate immune priming in the Lophotrochozoan Pacific oyster, Crassostrea gigas Lafont, Maxime Petton, Bruno Vergnes, Agnès Pauletto, Marianna Segarra, Amélie Gourbal, Benjamin Montagnani, Caroline Interactions Hôtes-Pathogènes-Environnements (IHPE) Université de Perpignan Via Domitia (UPVD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM) Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR) Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM) Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) Università degli Studi di Padova = University of Padua (Unipd) ANR-14-CE19-0023,DECIPHER,Déchiffrage des maladies multifactorielles: cas des mortalités de l'huître(2014) 2017-10-13 https://hal.science/hal-01632346 https://hal.science/hal-01632346/document https://hal.science/hal-01632346/file/Lafont-2017-SciRep.LongLasting.pdf https://doi.org/10.1038/s41598-017-13564-0 en eng HAL CCSD Nature Publishing Group info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-017-13564-0 hal-01632346 https://hal.science/hal-01632346 https://hal.science/hal-01632346/document https://hal.science/hal-01632346/file/Lafont-2017-SciRep.LongLasting.pdf doi:10.1038/s41598-017-13564-0 info:eu-repo/semantics/OpenAccess ISSN: 2045-2322 EISSN: 2045-2322 Scientific Reports https://hal.science/hal-01632346 Scientific Reports, 2017, 7, pp.13143. ⟨10.1038/s41598-017-13564-0⟩ MEMORY INFECTION IN-VIVO INVERTEBRATE IMMUNITY DOUBLE-STRANDED-RNA SPOT SYNDROME VIRUS OSTREID HERPESVIRUS-1 SECONDARY CHALLENGE VIBRIO-SPLENDIDUS RESPONSES ACL [SDV.IMM.II]Life Sciences [q-bio]/Immunology/Innate immunity [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology [SDE.BE]Environmental Sciences/Biodiversity and Ecology info:eu-repo/semantics/article Journal articles 2017 ftanrparis https://doi.org/10.1038/s41598-017-13564-0 2023-11-18T22:37:28Z International audience In the last decade, a paradigm shift has emerged in comparative immunology. Invertebrates can no longer be considered to be devoid of specific recognition and immune memory. However, we still lack a comprehensive view of these phenomena and their molecular mechanisms across phyla, especially in terms of duration, specificity, and efficiency in a natural context. In this study, we focused on a Lophotrochozoan/virus interaction, as antiviral priming is mostly overlooked in molluscs. Juvenile Crassostrea gigas oysters experience reoccurring mass mortalities events from Ostreid herpes virus 1 with no existing therapeutic treatment. Our results showed that various nucleic acid injections can prime oysters to trigger an antiviral state ultimately protecting them against a subsequent viral infection. Focusing on poly(I:C) as elicitor, we evidenced that it protected from an environmental infection, by mitigating viral replication. That protection seemed to induce a specific antiviral response as poly(I:C) fails to protect against a pathogenic bacteria. Finally, we showed that this phenomenon was long-lasting, persisting for at least 5 months thus suggesting for the first time the existence of innate immune memory in this invertebrate species. This study strengthens the emerging hypotheses about the broad conservation of innate immune priming and memory mechanisms in Lophotrochozoans. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Portail HAL-ANR (Agence Nationale de la Recherche) Pacific Scientific Reports 7 1 |