Microencapsulation of Piscirickettsia salmonis Antigens for Fish Oral Immunization: Optimization and Stability Studies
The development of fish oral vaccines is of great interest to the aquaculture industry due to the possibility of rapid vaccination of a large number of animals at reduced cost. In a previous study, we evaluated the effect of alginate-encapsulated Piscirickettsia salmonis antigens (AEPSA) incorporate...
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ftanid:oai:repositorio.anid.cl:10533/78679 2024-09-15T17:56:29+00:00 Microencapsulation of Piscirickettsia salmonis Antigens for Fish Oral Immunization: Optimization and Stability Studies Sotomayor-Gerding, Daniela Miguel Troncoso, Jose Diaz-Riquelme, Katherine Mariana Torres-Obreque, Karin Cumilaf, Juan Yanez, Alejandro J. Rubilar, Monica 2024-08-26T17:16:50Z PDF https://hdl.handle.net/10533/78679 https://doi.org/10.3390/polym14235115 https://repositorio.ufro.cl/s/repositorio-ufro/item/4770 en eng doi:10.3390/polym14235115 https://repositorio.ufro.cl/s/repositorio-ufro/item/4770 https://hdl.handle.net/10533/78679 POLYMERS Piscirickettsia salmonis oral vaccine alginate fish feed vacuum coating particle size encapsulation efficiency antigen stability Micro-CT-scanning artículo original 2024 ftanid https://doi.org/10.3390/polym14235115 2024-09-01T23:42:49Z The development of fish oral vaccines is of great interest to the aquaculture industry due to the possibility of rapid vaccination of a large number of animals at reduced cost. In a previous study, we evaluated the effect of alginate-encapsulated Piscirickettsia salmonis antigens (AEPSA) incorporated in feed, effectively enhancing the immune response in Atlantic salmon (Salmo salar). In this study, we seek to characterize AEPSA produced by ionic gelation using an aerodynamically assisted jetting (AAJ) system, to optimize microencapsulation efficiency (EE%), to assess microparticle stability against environmental (pH, salinity and temperature) and gastrointestinal conditions, and to evaluate microparticle incorporation in fish feed pellets through micro-CT-scanning. The AAJ system was effective in obtaining small microparticles (d < 20 mu m) with a high EE% (97.92%). Environmental conditions (pH, salinity and temperature) generated instability in the microparticles, triggering protein release. 62.42% of the protein content was delivered at the intestinal level after in vitro digestion. Finally, micro-CT-scanning images confirmed microparticle incorporation in fish feed pellets. In conclusion, the AAJ system is effective at encapsulating P. salmonis antigens in alginate with a high EE% and a size small enough to be incorporated in fish feed and produce an oral vaccine. Article in Journal/Newspaper Atlantic salmon Salmo salar Repositorio ANID (Agencia Nacional de Investigación y Desarrollo) Polymers 14 23 5115 |
institution |
Open Polar |
collection |
Repositorio ANID (Agencia Nacional de Investigación y Desarrollo) |
op_collection_id |
ftanid |
language |
English |
topic |
Piscirickettsia salmonis oral vaccine alginate fish feed vacuum coating particle size encapsulation efficiency antigen stability Micro-CT-scanning |
spellingShingle |
Piscirickettsia salmonis oral vaccine alginate fish feed vacuum coating particle size encapsulation efficiency antigen stability Micro-CT-scanning Sotomayor-Gerding, Daniela Microencapsulation of Piscirickettsia salmonis Antigens for Fish Oral Immunization: Optimization and Stability Studies |
topic_facet |
Piscirickettsia salmonis oral vaccine alginate fish feed vacuum coating particle size encapsulation efficiency antigen stability Micro-CT-scanning |
description |
The development of fish oral vaccines is of great interest to the aquaculture industry due to the possibility of rapid vaccination of a large number of animals at reduced cost. In a previous study, we evaluated the effect of alginate-encapsulated Piscirickettsia salmonis antigens (AEPSA) incorporated in feed, effectively enhancing the immune response in Atlantic salmon (Salmo salar). In this study, we seek to characterize AEPSA produced by ionic gelation using an aerodynamically assisted jetting (AAJ) system, to optimize microencapsulation efficiency (EE%), to assess microparticle stability against environmental (pH, salinity and temperature) and gastrointestinal conditions, and to evaluate microparticle incorporation in fish feed pellets through micro-CT-scanning. The AAJ system was effective in obtaining small microparticles (d < 20 mu m) with a high EE% (97.92%). Environmental conditions (pH, salinity and temperature) generated instability in the microparticles, triggering protein release. 62.42% of the protein content was delivered at the intestinal level after in vitro digestion. Finally, micro-CT-scanning images confirmed microparticle incorporation in fish feed pellets. In conclusion, the AAJ system is effective at encapsulating P. salmonis antigens in alginate with a high EE% and a size small enough to be incorporated in fish feed and produce an oral vaccine. |
author2 |
Miguel Troncoso, Jose Diaz-Riquelme, Katherine Mariana Torres-Obreque, Karin Cumilaf, Juan Yanez, Alejandro J. Rubilar, Monica |
format |
Article in Journal/Newspaper |
author |
Sotomayor-Gerding, Daniela |
author_facet |
Sotomayor-Gerding, Daniela |
author_sort |
Sotomayor-Gerding, Daniela |
title |
Microencapsulation of Piscirickettsia salmonis Antigens for Fish Oral Immunization: Optimization and Stability Studies |
title_short |
Microencapsulation of Piscirickettsia salmonis Antigens for Fish Oral Immunization: Optimization and Stability Studies |
title_full |
Microencapsulation of Piscirickettsia salmonis Antigens for Fish Oral Immunization: Optimization and Stability Studies |
title_fullStr |
Microencapsulation of Piscirickettsia salmonis Antigens for Fish Oral Immunization: Optimization and Stability Studies |
title_full_unstemmed |
Microencapsulation of Piscirickettsia salmonis Antigens for Fish Oral Immunization: Optimization and Stability Studies |
title_sort |
microencapsulation of piscirickettsia salmonis antigens for fish oral immunization: optimization and stability studies |
publishDate |
2024 |
url |
https://hdl.handle.net/10533/78679 https://doi.org/10.3390/polym14235115 https://repositorio.ufro.cl/s/repositorio-ufro/item/4770 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
POLYMERS |
op_relation |
doi:10.3390/polym14235115 https://repositorio.ufro.cl/s/repositorio-ufro/item/4770 https://hdl.handle.net/10533/78679 |
op_doi |
https://doi.org/10.3390/polym14235115 |
container_title |
Polymers |
container_volume |
14 |
container_issue |
23 |
container_start_page |
5115 |
_version_ |
1810432683073863680 |