Proteome data of the raw and the heated Pacific oyster (Crassostrea gigas)

• Other Authors: Ludwig Ludwig Lopata (hasAssociationWith), Ludwig Ludwig Lopata (hasCollector), Roni Benjamin Krieser (hasAssociationWith), Roni Benjamin Krieser (hasCollector), Sandip Kamath (hasCollector), Sandip Kamath (hasAssociationWith)
• Format: Dataset
• Language: unknown
• Published: James Cook University
• Subjects: Pacific oyster; proteome; mass spectrometry; Crassostrea gigas
• Online Access: https://researchdata.edu.au/proteome-raw-heated-crassostrea-gigas/815652; https://researchdata.jcu.edu.au//published/8a0dc4110b405eac8e6e7be6400f1576; https://doi.org/10.4225/28/588eb674aa910

Protein compositions of the Pacific oyster extracts were identified through bottom-up mass spectrometry approach. The proteins were separated on an SDS-PAGE gel, cut into small pieces and tryptic digested (Fig 3A). The peptide mixtures were then subjected to LC-MS/MS analysis generating a total of 81831 spectra for the raw extract and 88130 spectra for the heated extract. Mascot searches were conducted against Pacific oyster proteomes, and the identified proteins were validated by Scaffold (version Scaffold_4.7.3) using criteria at least two peptides at a 99.0% probability. In total Scaffold identified 1,086 proteins from raw extract and 130 proteins from the heated extract, with 103 proteins are shared between the two extracts. This dataset consists of a spreadsheet in MS Excel (.xlsx) and Open Document formats (.ods) These datasets contain proteins identified by mass spectrometry in the raw and the heated sample of Pacific oyster (C. gigas)