Abatus Microsatellites data set

• Other Authors: AADC (originator), AU/AADC > Australian Antarctic Data Centre, Australia (resourceProvider)
• Format: Dataset
• Language: unknown
• Published: Australian Ocean Data Network
• Subjects: biota; environment; oceans; SEA URCHINS; EARTH SCIENCE; BIOLOGICAL CLASSIFICATION; ANIMALS/INVERTEBRATES; ECHINODERMS; CONTAMINANT LEVELS/SPILLS; HUMAN DIMENSIONS; ENVIRONMENTAL IMPACTS; OCEAN CONTAMINANTS; WATER QUALITY; SEWAGE DISPOSAL; OUTFALL PIPE - DAVIS STATION; ADS > Automated DNA Sequencer; FIELD INVESTIGATION; FIELD SURVEYS; LABORATORY; AMD/AU; AMD; CEOS; GEOGRAPHIC REGION > POLAR; Antarctic; Coulter; Davis Station; Davis-Station; East Antarctica; Vestfold; Vestfold Hills; Antarc*; Antarctica
• Online Access: https://researchdata.ands.org.au/abatus-microsatellites-set/754222; https://data.aad.gov.au/metadata/records/AAS_3051_AbatusMicrosatellites; https://data.aad.gov.au/eds/4683/download; https://data.aad.gov.au/eds/4684/download; https://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_3051_AbatusMicrosatellites; https://secure3.aad.gov.au/proms/public/projects/report_project_public.cfm?project_no=AAS_3051

Samples from sampling sites OF1 and OF2 were collected during the 2009/2010 summer season. Samples from sampling sites OF, OF3 and OF4 were collected during the 2012/2013 summer season. Depths at which samples were collected: OF1: 4.5-7.0 m deep OF2: 10-12 m deep OF, OF3, OF4 equal or less than 4 m deep. Original datasheet was reformatted to fit IPT Biodiversity.AQ standard. The new datasheet "AbatusUrchin_microsatellites.csv" provides the dataset ID, verbatim latitude and verbatim longitude that were converted to decimal latitude and longitude, also provided. Taxonomical organisation to the lowest taxonomical rank that could be determined, after matched in WoRMS (World Register of Marine Species). Also provides other parameters such as localisation, sampling depth, occurrence, and date of event. The present data set corresponds to the genotypes for seven microsatellite markers for three Antarctic sea urchin species of the genus Abatus. Sea urchin individuals were collected in five sites separated by up to 5 km in the near-shore area surrounding Davis Station in the Vestfold Hills Region, East Antarctica. For each microsatellite loci, the size of each allele was scored (in base pairs) using the CEQ 8000 Genetic Analysis System software v.8.0. Fragments were separated on an automated sequencer (CEQ 8000, Beckman Coulter) in the Central Science Laboratory at University of Tasmania. Main goal was to study and compare the fine-scale population structure and demographic history of three species of Antarctic sea urchins (Abatus ingens, Abatus shackletoni and Abatus philippii) in the near-shore area surrounding the Australian Reseach Sation Davis in East Antarctica. A secondary goal was to test potential impacts of pollution from the Davis Station wastewater discharge on the genetic diversity and effective population size of these species.