2010/11 VMS Geonomics sampling - data collected from the VMS (Voyage Marine Science) voyage of the Aurora Australis

• Other Authors: WILLIAMS, TIM (hasPrincipalInvestigator), WILLIAMS, TIM (processor), WILKINS, DAVID (hasPrincipalInvestigator), WILKINS, DAVID (processor), Australian Antarctic Data Centre (publisher)
• Format: Dataset
• Language: unknown
• Published: Australian Antarctic Data Centre
• Subjects: oceans; MARINE ENVIRONMENT MONITORING; EARTH SCIENCE; PROTISTS; BIOLOGICAL CLASSIFICATION; VMS; Genomics; CTD > Conductivity; Temperature; Depth; NISKIN BOTTLES; SHIPS; R/V AA > R/V Aurora Australis; OCEAN > SOUTHERN OCEAN > Mertz Polynya; CONTINENT > ANTARCTICA; GEOGRAPHIC REGION > POLAR; Southern Ocean; Antarc*; Antarctica; aurora australis
• Online Access: https://researchdata.ands.org.au/201011-vms-geonomics-aurora-australis/702006; https://doi.org/10.4225/15/59a601e430067; https://data.aad.gov.au/metadata/records/VMS_Genomics; http://nla.gov.au/nla.party-617536

Purpose of future metagenomic (DNA), metaproteomic (protein) and metatranscriptomic (RNA) analysis: For each sample, two drums (~200L each) of seawater were collected. Samples were taken from CTD sites, and surface samples (2m depth) taken at each of these sites. At most of these CTD sites, a deeper sample was taken according to the location of the DCM at that site. The 200L seawater is pumped through a 20 micron mesh to remove the largest particles, then the biomass is collected on three consecutive filters corresponding to decreasing pore size (3.0 microns, 0.8 microns, 0.1 microns). This is repeated for each sample using the second 200L of seawater to generate duplicates for each sample. The overall aim is to determine the identity of microbes present in the Southern Ocean, and what microbial metabolic processes are in operation. In other words: who is there, and what they are doing. Special emphasis was placed on the SR3 transect. Samples were collected as below. For each sample, a total of six filters were obtained (3x pore sizes, 2x replicates). Each filter is stored in a storage buffer in a 50mL tube, and placed at -80 degrees C for the remainder of the voyage.