2010/11 VMS Geonomics sampling - data collected from the VMS (Voyage Marine Science) voyage of the Aurora Australis

Purpose of future metagenomic (DNA), metaproteomic (protein) and metatranscriptomic (RNA) analysis: For each sample, two drums (~200L each) of seawater were collected. Samples were taken from CTD sites, and surface samples (2m depth) taken at each of these sites. At most of these CTD sites, a deeper...

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Bibliographic Details
Other Authors: WILLIAMS, TIM (hasPrincipalInvestigator), WILLIAMS, TIM (processor), WILKINS, DAVID (hasPrincipalInvestigator), WILKINS, DAVID (processor), Australian Antarctic Data Centre (publisher)
Format: Dataset
Language:unknown
Published: Australian Antarctic Data Centre
Subjects:
oceans
MARINE ENVIRONMENT MONITORING
EARTH SCIENCE
PROTISTS
BIOLOGICAL CLASSIFICATION
VMS
Genomics
CTD &gt
Conductivity
Temperature
Depth
NISKIN BOTTLES
SHIPS
R/V AA &gt
R/V Aurora Australis
OCEAN &gt
SOUTHERN OCEAN &gt
Mertz Polynya
CONTINENT &gt
ANTARCTICA
GEOGRAPHIC REGION &gt
POLAR
Southern Ocean
Antarc*
Antarctica
aurora australis
Online Access:https://researchdata.ands.org.au/201011-vms-geonomics-aurora-australis/702006
https://doi.org/10.4225/15/59a601e430067
https://data.aad.gov.au/metadata/records/VMS_Genomics
http://nla.gov.au/nla.party-617536
Description
Summary:Purpose of future metagenomic (DNA), metaproteomic (protein) and metatranscriptomic (RNA) analysis: For each sample, two drums (~200L each) of seawater were collected. Samples were taken from CTD sites, and surface samples (2m depth) taken at each of these sites. At most of these CTD sites, a deeper sample was taken according to the location of the DCM at that site. The 200L seawater is pumped through a 20 micron mesh to remove the largest particles, then the biomass is collected on three consecutive filters corresponding to decreasing pore size (3.0 microns, 0.8 microns, 0.1 microns). This is repeated for each sample using the second 200L of seawater to generate duplicates for each sample. The overall aim is to determine the identity of microbes present in the Southern Ocean, and what microbial metabolic processes are in operation. In other words: who is there, and what they are doing. Special emphasis was placed on the SR3 transect. Samples were collected as below. For each sample, a total of six filters were obtained (3x pore sizes, 2x replicates). Each filter is stored in a storage buffer in a 50mL tube, and placed at -80 degrees C for the remainder of the voyage.

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