Total and hydrocarbon-degrading bacteria in the permeable reactive barrier at Casey

• Other Authors: POWELL, SHANE (hasPrincipalInvestigator), BOWMAN, JOHN (hasPrincipalInvestigator), RAYNER, JOHN LENDON (processor), Australian Antarctic Data Centre (publisher)
• Format: Dataset
• Language: unknown
• Published: Australian Antarctic Data Centre
• Subjects: environment; TERRESTRIAL ECOSYSTEMS; EARTH SCIENCE; BIOSPHERE; CONTAMINANT LEVELS/SPILLS; HUMAN DIMENSIONS; ENVIRONMENTAL IMPACTS; OIL SPILLS; CARBON; LAND SURFACE; SOILS; RECLAMATION/REVEGETATION/RESTORATION; PETROLEUM; SOLID EARTH; EARTH GASES/LIQUIDS; BACTERIA/ARCHAEA; BIOLOGICAL CLASSIFICATION; hydrocarboon biodegradation; zeolite; fuel spill; bioremediation; permeable reactive barrier; granulated activated carbon; heterotrophic bacteria; diesel; special antarctic blend; TRAPS; FIELD SURVEYS; LABORATORY; CONTINENT > ANTARCTICA > Casey; GEOGRAPHIC REGION > POLAR; Antarctic; Casey Station; Bushnell; Antarc*; Antarctica
• Online Access: https://researchdata.ands.org.au/total-hydrocarbon-degrading-barrier-casey/701464; https://doi.org/10.26179/5c9b0907756dd; https://data.aad.gov.au/metadata/records/PRB_bacterialnumbers_2007; http://nla.gov.au/nla.party-617536

Material was collected from the permeable reactive barrier that was installed at the Main Power House spill site at Casey Station, Antarctica. Material from the barrier was collected from 40 sites throughout the barrier and at varying depths. Samples of the raw material GAC (granulated activated carbon) and Zeopro and three soil samples collected upgradient from the barrier are also included. The numbers of total heterotrophic bacteria were determined using a most-probable-number count procedure with half-strength tryptone-soya broth and the numbers of Special Antarctic diesel - degrading bacteria were determined using Bushnell-Haas medium with SAB as the sole carbon source. Numbers are presented as the most-probable number per gram of wet barrier material. This work was completed as part of ASAC projects 1163 and 2672 (ASAC_1163, ASAC_2672). More information about the dataset is presented on the summary worksheet of the download file - this information is copied below: Most probable number counts were carried out on material sampled from the permeable reactive barrier at Casey in Jan/Feb 2007. 4-tube MPN with three replicates for each sample. Most probable number was calculated using the MPN Calculator available from: http:/members.ync.net/mcuriale/mpn/index.html The barcode numbers are barcodes recorded in the Human Impacts Sample Tracking Database. The sample name has the multiport location and depth of sample in m (eg MP01 0.45 is multiport 1 at 0.45m depth). Total heterotrophs were estimated using half-strength tryptone-soya broth in 96-well titre trays. 1:10 serial dilutions were made in a total of 200 microlitres of medium. Plates were incubated at 10°C for 7 days. Plates were scored manually with visually turbid cells being positive. SAB-degrading bacteria were estimated using Bushnell-Haas broth and 5ul of SAB as carbon source. 1:10 serial dilutions were made in a total of 200 microlitres of medium. Plates were incubated at 10 degrees C for 14 days. At this time 40 microlitres of INT solution were added and incubated for another two days (INT = 2.25 g/l of iodonitrotetrazolium chloride). Plates were scored manually, with the presence of a red precipitate or red colour being positive.