Microbial data from the Macquarie Island Respirometry Experiment 2006

• Other Authors: BOWMAN, JOHN (hasPrincipalInvestigator), POWELL, SHANE (processor), Australian Antarctic Data Centre (publisher)
• Format: Dataset
• Language: unknown
• Published: Australian Antarctic Data Centre
• Subjects: environment; OIL SPILLS; EARTH SCIENCE; HUMAN DIMENSIONS; ENVIRONMENTAL IMPACTS; SOILS; LAND SURFACE; real-time PCR; hydrocarbon biodegradation; denitrification; sub-Antarctic; LABORATORY; OCEAN > SOUTHERN OCEAN; SOUTHERN OCEAN > MACQUARIE ISLAND; GEOGRAPHIC REGION > POLAR; Antarctic; Southern Ocean; Antarc*; Macquarie Island
• Online Access: https://researchdata.ands.org.au/microbial-macquarie-island-experiment-2006/701409; https://doi.org/10.4225/15/59b1dd61113c5; https://data.aad.gov.au/metadata/records/MI_microcosm2006_microbial_data; http://nla.gov.au/nla.party-617536

A microcosm experiment utilising a respirometry system and 14C-labelled hexadecane was initiated to investigate the effects of differing oxygen regimes on hydrocarbon degradation in soil from sub-Antarctic Macquarie Island. Measurements of oxygen consumed, carbon dioxide produced, total petroleum hydrocarbon degradation and nitrate and ammonium concentrations were made. The microbial community structure at the start of the experiment and after 4, 8 and 12 weeks incubation was explored using terminal restriction fragment length polymorphism and real-time PCR quantification of alkane mono-oxygenase, napthalene dioxygenase, nitrous oxide reductase and ribosomal polymerase sub-unitB. The data described here are the microbial community data only. The download file contains an excel spreadsheet. The first sheet provides further information about the dataset. This work was part of AAS projects 2672 and 1163.