Alkane mono-oxygenase clone library from Macquarie Island soil

• Other Authors: POWELL, SHANE (hasPrincipalInvestigator), POWELL, SHANE (processor), BOWMAN, JOHN (hasPrincipalInvestigator), Australian Antarctic Data Centre (publisher)
• Format: Dataset
• Language: unknown
• Published: Australian Antarctic Data Centre
• Subjects: environment; ISLANDS; EARTH SCIENCE; BIOSPHERE; TERRESTRIAL ECOSYSTEMS; SOILS; LAND SURFACE; COMMUNITY STRUCTURE; ECOLOGICAL DYNAMICS; COMMUNITY DYNAMICS; microbial ecology; alkane mono-oxygenase alkB; ADS > Automated DNA Sequencer; LABORATORY; FIELD INVESTIGATION; OCEAN > SOUTHERN OCEAN; SOUTHERN OCEAN > MACQUARIE ISLAND; GEOGRAPHIC REGION > POLAR; Antarctic; Southern Ocean; Antarc*; Macquarie Island
• Online Access: https://researchdata.ands.org.au/alkane-mono-oxygenase-island-soil/701404; https://doi.org/10.4225/15/59b1fea4da5c2; https://data.aad.gov.au/metadata/records/MI_alk_clones; http://nla.gov.au/nla.party-617536

This dataset consists of 81 DNA sequences of the alkane mono-oxygenase gene. The sequence data are in FASTA format which can be opened with any word-processing or sequence analysis software. The clone library was created using the primers described by Kloos et al. (2006, Journal Microbiological Methods 66:486-496) F: AAYACNGCNCAYGARCTNGGNCAYAA and R:GCRTGRTGRTCNGARTGNCGYTG. The library was created from a soil sample collected at the Main Powerhouse on Macquarie Island and is Human Impacts Sample Tracking Database barcode number:52774. These data were collected as part of AAS project 2672 - Pathways of alkane biodegradation in antarctic and subantarctic soils and sediments.