Sampling of planktonic microorganisms for metagenomic, metaproteomic and metatranscriptomic analyses

• Other Authors: CAVICCHIOLI, RICK (hasPrincipalInvestigator), HOFFMAN, JEFF (processor), MCQUAID, JEFF (processor), Australian Antarctic Data Centre (publisher)
• Format: Dataset
• Language: unknown
• Published: Australian Antarctic Data Centre
• Subjects: biota; oceans; PHYTOPLANKTON; EARTH SCIENCE; BIOSPHERE; AQUATIC ECOSYSTEMS; PLANKTON; SHIPS; R/V AA > R/V Aurora Australis; IPY > INTERNATIONAL POLAR YEAR; CASO > Climate of Antarctica and the Southern Ocean; OCEAN > SOUTHERN OCEAN; GEOGRAPHIC REGION > POLAR; Antarctic; Southern Ocean; Antarc*; Antarctica; aurora australis; International Polar Year; IPY; Sea ice
• Online Access: https://researchdata.ands.org.au/sampling-planktonic-microorganisms-metatranscriptomic-analyses/700768; https://data.aad.gov.au/metadata/records/CEAMARC_CASO_200708_V3_PLANKTON_GENOMICS_PROTEOMICS; http://nla.gov.au/nla.party-617536

Large volumes of water (200 - 500 L) were filtered and fractionated by size for various planktonic components: eukaryotic phytoplankton, prokaryotic picoplankton, and marine viruses. Sample sites were chosen to generate the widest diversity, and included planktonic blooms, oligotrophic zones, small polynyas near sea ice, nearshore areas, and Antarctic bottom water from coastal, canyon and deepwater areas. Half of each sample will be used for DNA library construction, and the other half will be used for meta-proteomic analysis. Random shotgun sequencing of the marine genomic libraries should produce a metagenomic snapshot of planktonic life in a variety of marine habitats. This work was completed as part of ASAC project 2899 (ASAC_2899).