Molecular Characterisation of Cold-Adapted Metallo-Oxotransferases from the Dimethylsulfoxide Reductase Family Expressed by Antarctic Bacteria

• Other Authors: CODD, RACHEL (hasPrincipalInvestigator), CODD, RACHEL (processor), Australian Antarctic Data Centre (publisher)
• Format: Dataset
• Language: unknown
• Published: Australian Antarctic Data Centre
• Subjects: biota; oceans; BACTERIA/ARCHAEA; EARTH SCIENCE; BIOLOGICAL CLASSIFICATION; ACAM; BACTERIA; MICROSCOPES; LABORATORY; CONTINENT > ANTARCTICA; GEOGRAPHIC REGION > POLAR; Antarctic; The Antarctic; Antarc*; Antarctica; Sea ice
• Online Access: https://researchdata.ands.org.au/molecular-characterisation-cold-antarctic-bacteria/699940; https://data.aad.gov.au/metadata/records/ASAC_2547; http://nla.gov.au/nla.party-617536

Metadata record for data from ASAC Project 2547 See the link below for public details on this project. Pue (greater than 90% as determined by SDS-PAGE) samples of nitrate reductase have been isolated from the Antarctic bacterium, Shewanella gelidimarina (ACAM 456T; Accession number U85907 (16S rDNA)). The protein is ~90 kDa (similar to nitrate reductase enzymes characterised from alternate bacteria) and stains positive in an in-situ nitrate reduction (native) assay technique. The protein may be N-terminal blocked, although further sequencing experiments are required to confirm this. This work is based upon phenotyped Antarctic bacteria (S. gelidimarina; S.frigidimarina) that was collected during other ASAC projects. (Refer: Psychrophilic Bacteria from Antarctic Sea-ice and Phospholipids of Antarctic sea ice algal communities new sources of PUFA [ASAC_708] and Biodiversity and ecophysiology of Antarctic sea-ice bacteria [ASAC_1012]). The download file contains 4 scientific papers produced from this work - one of these papers also contains a large set of accession numbers for data stored at GenBank.