Exopolysaccharides from Antarctic bacteria
Exopolysaccharide (EPS) is complex sugar made by many microbes in the Antarctic marine environment. This project seeks to understand the ecological role of microbial EPS in the Southern Ocean, where it is known to strongly influence primary production. We will investigate the chemical composition an...
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Format: | Dataset |
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Australian Antarctic Data Centre
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Online Access: | https://researchdata.edu.au/exopolysaccharides-antarctic-bacteria/699870 https://doi.org/10.4225/15/574BBB8462E3F https://data.aad.gov.au/metadata/records/ASAC_2348 http://nla.gov.au/nla.party-617536 |
id |
ftands:oai:ands.org.au::699870 |
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record_format |
openpolar |
institution |
Open Polar |
collection |
Research Data Australia (Australian National Data Service - ANDS) |
op_collection_id |
ftands |
language |
unknown |
topic |
biota oceans PACK ICE EARTH SCIENCE CRYOSPHERE SEA ICE TRACE METALS TERRESTRIAL HYDROSPHERE WATER QUALITY/WATER CHEMISTRY CARBON OCEAN CHEMISTRY NUTRIENTS ORGANIC CARBON ORGANIC MATTER TRACE ELEMENTS BACTERIA/ARCHAEA BIOLOGICAL CLASSIFICATION EARTH SCIENCE > BIOSPHERE > ECOSYSTEMS > MARINE ECOSYSTEMS > COASTAL PELAGIC SPECIES COMPETITION BIOSPHERE ECOLOGICAL DYNAMICS SPECIES/POPULATION INTERACTIONS EVOLUTIONARY ADAPTATION SURVIVAL RATES BIOGEOCHEMICAL CYCLES ECOSYSTEM FUNCTIONS FOOD-WEB DYNAMICS NUTRIENT CYCLING DECOMPOSITION BIOMASS DYNAMICS SECONDARY PRODUCTION BIOAVAILABILITY ECOTOXICOLOGY BACTERIA BIODIVERSITY BRINE CHANNELS CARBOHYDRATE CHEMISTRY COLD ADAPTATION EPS EXOPOLYSACCHARIDES FLAVOBACTERIA IRON LIGANDS PSEUDOALTEROMONAS PSYCHROPHILIC BACTERIA NISKIN BOTTLES PLANKTON NETS MICROSCOPES SPECTROPHOTOMETERS FTIR SPECTROMETER > Fourier Transform Infrared Spectrometer Spectrometers GC-MS > Gas Chromatograph/Mass Spectrometry OCEAN > SOUTHERN OCEAN CONTINENT > ANTARCTICA GEOGRAPHIC REGION > POLAR |
spellingShingle |
biota oceans PACK ICE EARTH SCIENCE CRYOSPHERE SEA ICE TRACE METALS TERRESTRIAL HYDROSPHERE WATER QUALITY/WATER CHEMISTRY CARBON OCEAN CHEMISTRY NUTRIENTS ORGANIC CARBON ORGANIC MATTER TRACE ELEMENTS BACTERIA/ARCHAEA BIOLOGICAL CLASSIFICATION EARTH SCIENCE > BIOSPHERE > ECOSYSTEMS > MARINE ECOSYSTEMS > COASTAL PELAGIC SPECIES COMPETITION BIOSPHERE ECOLOGICAL DYNAMICS SPECIES/POPULATION INTERACTIONS EVOLUTIONARY ADAPTATION SURVIVAL RATES BIOGEOCHEMICAL CYCLES ECOSYSTEM FUNCTIONS FOOD-WEB DYNAMICS NUTRIENT CYCLING DECOMPOSITION BIOMASS DYNAMICS SECONDARY PRODUCTION BIOAVAILABILITY ECOTOXICOLOGY BACTERIA BIODIVERSITY BRINE CHANNELS CARBOHYDRATE CHEMISTRY COLD ADAPTATION EPS EXOPOLYSACCHARIDES FLAVOBACTERIA IRON LIGANDS PSEUDOALTEROMONAS PSYCHROPHILIC BACTERIA NISKIN BOTTLES PLANKTON NETS MICROSCOPES SPECTROPHOTOMETERS FTIR SPECTROMETER > Fourier Transform Infrared Spectrometer Spectrometers GC-MS > Gas Chromatograph/Mass Spectrometry OCEAN > SOUTHERN OCEAN CONTINENT > ANTARCTICA GEOGRAPHIC REGION > POLAR Exopolysaccharides from Antarctic bacteria |
topic_facet |
biota oceans PACK ICE EARTH SCIENCE CRYOSPHERE SEA ICE TRACE METALS TERRESTRIAL HYDROSPHERE WATER QUALITY/WATER CHEMISTRY CARBON OCEAN CHEMISTRY NUTRIENTS ORGANIC CARBON ORGANIC MATTER TRACE ELEMENTS BACTERIA/ARCHAEA BIOLOGICAL CLASSIFICATION EARTH SCIENCE > BIOSPHERE > ECOSYSTEMS > MARINE ECOSYSTEMS > COASTAL PELAGIC SPECIES COMPETITION BIOSPHERE ECOLOGICAL DYNAMICS SPECIES/POPULATION INTERACTIONS EVOLUTIONARY ADAPTATION SURVIVAL RATES BIOGEOCHEMICAL CYCLES ECOSYSTEM FUNCTIONS FOOD-WEB DYNAMICS NUTRIENT CYCLING DECOMPOSITION BIOMASS DYNAMICS SECONDARY PRODUCTION BIOAVAILABILITY ECOTOXICOLOGY BACTERIA BIODIVERSITY BRINE CHANNELS CARBOHYDRATE CHEMISTRY COLD ADAPTATION EPS EXOPOLYSACCHARIDES FLAVOBACTERIA IRON LIGANDS PSEUDOALTEROMONAS PSYCHROPHILIC BACTERIA NISKIN BOTTLES PLANKTON NETS MICROSCOPES SPECTROPHOTOMETERS FTIR SPECTROMETER > Fourier Transform Infrared Spectrometer Spectrometers GC-MS > Gas Chromatograph/Mass Spectrometry OCEAN > SOUTHERN OCEAN CONTINENT > ANTARCTICA GEOGRAPHIC REGION > POLAR |
description |
Exopolysaccharide (EPS) is complex sugar made by many microbes in the Antarctic marine environment. This project seeks to understand the ecological role of microbial EPS in the Southern Ocean, where it is known to strongly influence primary production. We will investigate the chemical composition and structure of EPS obtained from Antarctic microbes, which will improve our knowledge of its ecological significance and biotechnological potential. Dataset includes the following: 1) Information on Exopolysaccharide-producing bacterial isolates, isolation sites, media used and growth conditions. 2) 16S rRNA gene sequence and fatty acid data of isolates for strain identification. 3) Exopolysaccharide chemistry data including EPS carbohydrate composition, organic acid composition, sulfate content, molecular weight. 4) Physiology of exopolysaccharide synthesis. Effects of temperature and other factors on EPS yield and glucose conversion efficiency. 5) Iron binding properties. The download file includes: 11 files File 1. Bacterial isolate 16S rRNA gene sequences obtained from Southern Ocean seawater or ice samples. The sequences are all deposited on the GenBank nucleotide (NCBI) database. Sequences are in FASTA format. File 2. Seawater and sea-ice sample information including sites samples, sample type. File 3. Data for exopolysaccharide (EPS)-producing bacteria isolated and subsequently selected for further studied. Information indicates special treatments used to obtain strains including plankton towing, filtration method, and enrichment. Identification to species level was determined by 16S rRNA gene sequence analysis. File 4. EPS-producing bacterial isolate fatty acid content determined using GC/MS procedures. File 5. Basic chemical data for EPS from Antarctic isolates including protein, sulfate, and sugar type relative content (determined by chemical procedures), molecular weight in kilodaltons and polydispersity (determined by gel-based molecular seiving). File 6 Monosaccharide unit composition determined by GC/MS of EPS from Antarctic isolates. File 7. Effect of temperature on culture viscosity and growth of EPS-producing bacterium Pseudoalteromonas sp. CAM025 as affected by temperature. File 8. Effect of temperature on EPS and cell yields and EPS synthesis efficiency (as indicated by glucose consumption) of EPS-producing bacterium Pseudoalteromonas sp. CAM025 as affected by temperature. File 9. Efficiency of copper and cadmium metal ion adsorption onto EPS from EPS-producing bacterium Pseudoalteromonas sp. CAM025. File 10. Phenotypic characteristics data for novel EPS-producing Antarctic strain CAM030. Represents type strain of Olleya marilimosa. File 11. Effect of temperature on chemical make up of EPS from EPS-producing bacterium Pseudoalteromonas sp. CAM025. |
author2 |
BOWMAN, JOHN (hasPrincipalInvestigator) BOWMAN, JOHN (processor) Australian Antarctic Data Centre (publisher) |
format |
Dataset |
title |
Exopolysaccharides from Antarctic bacteria |
title_short |
Exopolysaccharides from Antarctic bacteria |
title_full |
Exopolysaccharides from Antarctic bacteria |
title_fullStr |
Exopolysaccharides from Antarctic bacteria |
title_full_unstemmed |
Exopolysaccharides from Antarctic bacteria |
title_sort |
exopolysaccharides from antarctic bacteria |
publisher |
Australian Antarctic Data Centre |
url |
https://researchdata.edu.au/exopolysaccharides-antarctic-bacteria/699870 https://doi.org/10.4225/15/574BBB8462E3F https://data.aad.gov.au/metadata/records/ASAC_2348 http://nla.gov.au/nla.party-617536 |
op_coverage |
Spatial: northlimit=-60.0; southlimit=-67.0; westlimit=70.0; eastLimit=145.0; projection=WGS84 Temporal: From 2003-07-01 to 2004-06-30 |
long_lat |
ENVELOPE(70.0,145.0,-60.0,-67.0) |
geographic |
Antarctic Southern Ocean The Antarctic |
geographic_facet |
Antarctic Southern Ocean The Antarctic |
genre |
Antarc* Antarctic Antarctica Sea ice Southern Ocean |
genre_facet |
Antarc* Antarctic Antarctica Sea ice Southern Ocean |
op_source |
Australian Antarctic Data Centre |
op_relation |
https://researchdata.edu.au/exopolysaccharides-antarctic-bacteria/699870 da4058f7-1afb-43b8-8e7e-ae01b8cfc76f doi:10.4225/15/574BBB8462E3F ASAC_2348 https://data.aad.gov.au/metadata/records/ASAC_2348 http://nla.gov.au/nla.party-617536 |
op_doi |
https://doi.org/10.4225/15/574BBB8462E3F |
_version_ |
1766146860962021376 |
spelling |
ftands:oai:ands.org.au::699870 2023-05-15T13:41:12+02:00 Exopolysaccharides from Antarctic bacteria BOWMAN, JOHN (hasPrincipalInvestigator) BOWMAN, JOHN (processor) Australian Antarctic Data Centre (publisher) Spatial: northlimit=-60.0; southlimit=-67.0; westlimit=70.0; eastLimit=145.0; projection=WGS84 Temporal: From 2003-07-01 to 2004-06-30 https://researchdata.edu.au/exopolysaccharides-antarctic-bacteria/699870 https://doi.org/10.4225/15/574BBB8462E3F https://data.aad.gov.au/metadata/records/ASAC_2348 http://nla.gov.au/nla.party-617536 unknown Australian Antarctic Data Centre https://researchdata.edu.au/exopolysaccharides-antarctic-bacteria/699870 da4058f7-1afb-43b8-8e7e-ae01b8cfc76f doi:10.4225/15/574BBB8462E3F ASAC_2348 https://data.aad.gov.au/metadata/records/ASAC_2348 http://nla.gov.au/nla.party-617536 Australian Antarctic Data Centre biota oceans PACK ICE EARTH SCIENCE CRYOSPHERE SEA ICE TRACE METALS TERRESTRIAL HYDROSPHERE WATER QUALITY/WATER CHEMISTRY CARBON OCEAN CHEMISTRY NUTRIENTS ORGANIC CARBON ORGANIC MATTER TRACE ELEMENTS BACTERIA/ARCHAEA BIOLOGICAL CLASSIFICATION EARTH SCIENCE > BIOSPHERE > ECOSYSTEMS > MARINE ECOSYSTEMS > COASTAL PELAGIC SPECIES COMPETITION BIOSPHERE ECOLOGICAL DYNAMICS SPECIES/POPULATION INTERACTIONS EVOLUTIONARY ADAPTATION SURVIVAL RATES BIOGEOCHEMICAL CYCLES ECOSYSTEM FUNCTIONS FOOD-WEB DYNAMICS NUTRIENT CYCLING DECOMPOSITION BIOMASS DYNAMICS SECONDARY PRODUCTION BIOAVAILABILITY ECOTOXICOLOGY BACTERIA BIODIVERSITY BRINE CHANNELS CARBOHYDRATE CHEMISTRY COLD ADAPTATION EPS EXOPOLYSACCHARIDES FLAVOBACTERIA IRON LIGANDS PSEUDOALTEROMONAS PSYCHROPHILIC BACTERIA NISKIN BOTTLES PLANKTON NETS MICROSCOPES SPECTROPHOTOMETERS FTIR SPECTROMETER > Fourier Transform Infrared Spectrometer Spectrometers GC-MS > Gas Chromatograph/Mass Spectrometry OCEAN > SOUTHERN OCEAN CONTINENT > ANTARCTICA GEOGRAPHIC REGION > POLAR dataset ftands https://doi.org/10.4225/15/574BBB8462E3F 2021-12-06T23:22:32Z Exopolysaccharide (EPS) is complex sugar made by many microbes in the Antarctic marine environment. This project seeks to understand the ecological role of microbial EPS in the Southern Ocean, where it is known to strongly influence primary production. We will investigate the chemical composition and structure of EPS obtained from Antarctic microbes, which will improve our knowledge of its ecological significance and biotechnological potential. Dataset includes the following: 1) Information on Exopolysaccharide-producing bacterial isolates, isolation sites, media used and growth conditions. 2) 16S rRNA gene sequence and fatty acid data of isolates for strain identification. 3) Exopolysaccharide chemistry data including EPS carbohydrate composition, organic acid composition, sulfate content, molecular weight. 4) Physiology of exopolysaccharide synthesis. Effects of temperature and other factors on EPS yield and glucose conversion efficiency. 5) Iron binding properties. The download file includes: 11 files File 1. Bacterial isolate 16S rRNA gene sequences obtained from Southern Ocean seawater or ice samples. The sequences are all deposited on the GenBank nucleotide (NCBI) database. Sequences are in FASTA format. File 2. Seawater and sea-ice sample information including sites samples, sample type. File 3. Data for exopolysaccharide (EPS)-producing bacteria isolated and subsequently selected for further studied. Information indicates special treatments used to obtain strains including plankton towing, filtration method, and enrichment. Identification to species level was determined by 16S rRNA gene sequence analysis. File 4. EPS-producing bacterial isolate fatty acid content determined using GC/MS procedures. File 5. Basic chemical data for EPS from Antarctic isolates including protein, sulfate, and sugar type relative content (determined by chemical procedures), molecular weight in kilodaltons and polydispersity (determined by gel-based molecular seiving). File 6 Monosaccharide unit composition determined by GC/MS of EPS from Antarctic isolates. File 7. Effect of temperature on culture viscosity and growth of EPS-producing bacterium Pseudoalteromonas sp. CAM025 as affected by temperature. File 8. Effect of temperature on EPS and cell yields and EPS synthesis efficiency (as indicated by glucose consumption) of EPS-producing bacterium Pseudoalteromonas sp. CAM025 as affected by temperature. File 9. Efficiency of copper and cadmium metal ion adsorption onto EPS from EPS-producing bacterium Pseudoalteromonas sp. CAM025. File 10. Phenotypic characteristics data for novel EPS-producing Antarctic strain CAM030. Represents type strain of Olleya marilimosa. File 11. Effect of temperature on chemical make up of EPS from EPS-producing bacterium Pseudoalteromonas sp. CAM025. Dataset Antarc* Antarctic Antarctica Sea ice Southern Ocean Research Data Australia (Australian National Data Service - ANDS) Antarctic Southern Ocean The Antarctic ENVELOPE(70.0,145.0,-60.0,-67.0) |