Operational Taxonomic Unit (OTU) Microbial Ecotoxicology data from Macquarie Island and Casey Station: TPH, Chemistry and OTU Abundance data

• Other Authors: SNAPE, IAN (hasPrincipalInvestigator), SNAPE, IAN (processor), SICILIANO, STEVEN (hasPrincipalInvestigator), SICILIANO, STEVEN (processor), WINSLEY, TRISTROM (processor), VAN DORST, JOSIE (processor), JI, MUKAN (processor), PALMER, ANNE (processor), LAGEREWSKIJ, GREG (processor), Australian Antarctic Data Centre (publisher)
• Format: Dataset
• Language: unknown
• Published: Australian Antarctic Data Centre
• Subjects: environment; CONTAMINANT LEVELS/SPILLS; EARTH SCIENCE; HUMAN DIMENSIONS; ENVIRONMENTAL IMPACTS; EARTH SCIENCE > TERRESTRIAL HYDROSPHERE > WATER QUALITY/WATER CHEMISTRY > CONTAMINANTS > PETROLEUM HYDROCARBONS; SOILS; LAND SURFACE; SOIL CHEMISTRY; TPH; Total petroleum hydrocarbons; CEC; Cation exchange capacity; XRF; X-ray fluorescence; GC; Gas Chromatography; GAS CHROMATOGRAPHS; FIELD INVESTIGATION; FIELD SURVEYS; LABORATORY; CONTINENT > NORTH AMERICA > UNITED STATES OF AMERICA > ALASKA; ANTARCTICA; CANADA; EUROPE > NORTHERN EUROPE > SCANDINAVIA > FINLAND; GREENLAND; ICELAND; MINNESOTA; CANADA > NEWFOUNDLAND AND LABRADOR; NORTHWEST TERRITORIES; NORWAY; NUNAVUT; GEOGRAPHIC REGION > POLAR; QUEBEC; SWEDEN; UNITED STATES OF AMERICA; YUKON TERRITORY; OCEAN > SOUTHERN OCEAN > MACQUARIE ISLAND; Newfoundland; Southern Ocean; Nunavut; Yukon; Northwest Territories; Canada; Greenland; Norway; Casey Station; Antarc*; Antarctica; Iceland; Macquarie Island; Alaska
• Online Access: https://researchdata.ands.org.au/operational-taxonomic-unit-otu-abundance/699472; https://doi.org/10.4225/15/54FFBE1DE34DF; https://data.aad.gov.au/metadata/records/ASAC_1163_Ecotox; http://nla.gov.au/nla.party-617536

This dataset contains the following information with regards to the Ecotox Project: General location and sampling date metadata. Soil characteristic analysis such as grain size, etc. Full breakdown of TPH (Total Petroleum Hydrocarbon) analysis generated through GC (Gas Chromatography) which includes individual compounds, ranges and ratios. Dry matter fraction and loss on ignition. Chemistry via water extraction, bicarb extraction and KCl extraction. CEC (Cation Exchange Capacity) exchangeable ion analysis. XRF (X-Ray Fluorescence) analysis Abundance matrices: This dataset contains information obtained by pyrosequencing genomic DNA extracts from soil with PCR primers targeting the bacterial 16S gene (27F/519R) and fungal ITS region (ITS1/ITS4-B). The data was processed in a pipeline using freely available 'mothur' software (v1.24.1). The reads were processed in 4 ways, this was a combination of subsampling the data to a number that normalised all but the 10 lowest samples and excluding operational taxonomic units (OTUs) that only occurred once in the entire dataset. This resulted in designations FULL_READS for the unsubsampled analyses, and SUBSAMPLED for those subsampled. For each of these combinations an OTU abundance matrix was generated which is a table of OTU by samples and the corresponding abundance of the OTU.