Biodiversity of Microbial mats in Antarctica

• Other Authors: LAYBOURN-PARRY, JOHANNA (hasPrincipalInvestigator), WILMOTTE, ANNICK (hasPrincipalInvestigator), WILMOTTE, ANNICK (processor), Australian Antarctic Data Centre (publisher)
• Format: Dataset
• Language: unknown
• Published: Australian Antarctic Data Centre
• Subjects: biota; inlandWaters; BACTERIA/ARCHAEA; EARTH SCIENCE; BIOLOGICAL CLASSIFICATION; MICROALGAE; PLANTS; EARTH SCIENCE > BIOSPHERE > ECOSYSTEMS > FRESHWATER ECOSYSTEMS > LAKE/POND; SALINE LAKES; BIOSPHERE; AQUATIC ECOSYSTEMS; LAKES; BIOMASS DYNAMICS; ECOLOGICAL DYNAMICS; ECOSYSTEM FUNCTIONS; COMMUNITY STRUCTURE; COMMUNITY DYNAMICS; ANTARCTICA; CELL LENGTH; CELL SHAPE; CELL WIDTH; CROSS-WALL CONSTRICTION; CROSS-WALL GRANULATION; CULTIVATION METHOD; CYANOBACTERIA; CYTOTOXICITY; FALSE BRANCHING; INTERNALLY TRANSCRIBED SPACER; ISOLATION MEDIA; ITS; LAKE; LATITUDE; LOCATION; LONGITUDE; MICROBIAL MATS; MICROMAT; MORPHOSPECIES; NECRIDIC CELL; OPERATIONAL TAXONOMIC UNIT; OTU; PHOTOSENSITIVITY; PRODUCTIVITY; REGION; SPECIES; STRAIN; TRICHOMES; VESTFOLD HILLS; FIELD SURVEYS; CONTINENT > ANTARCTICA > GEOGRAPHIC REGION > POLAR; Antarctic; Vestfold Hills; Vestfold; Davis Station; Davis-Station; Parry; Dyer; Antarc*; Antarctica; Australian Antarctic Division
• Online Access: https://researchdata.ands.org.au/biodiversity-microbial-mats-antarctica/699378; https://doi.org/10.4225/15/54851E6D9D265; https://data.aad.gov.au/metadata/records/ASAC_1049_Micromat; http://nla.gov.au/nla.party-617536

From the Micromat home page: Research on microbial biodiversity in Antarctica is still in a starting phase though it is a very promising area of research. Antarctica is characterised by its geographical and climatic isolation. The extreme climate has led to the evolution of novel biochemical adaptations to severe low temperatures and hypersalinity (in lakes), and possibly also of indigenous species. In addition, most of the continent has experienced little or no anthropogenic influence. This offers a unique opportunity to gather data on diversity of pristine biotopes. Diverse ice-covered lakes which include both freshwater and saline systems will be sampled during this project. Their bottom areas which receive sufficient solar radiation are covered by microbial mats dominated by cyanobacteria. Fossil layers of tens of thousands of years can be found in several lakes. The information on the mats is relatively spare. As we know now that only a small fraction of the true microbial diversity in natural environments has been observed and even less has been cultivated, this project will also assess the use of cultivation versus molecular methods to describe the biodiversity of these microbial mats for the different types of microorganisms present (Bacteria, Archaea, cyanobacteria, fungi, photosynthetic and heterotrophic protists). This part of the project: Sampling in the Vestfold Hills was carried out by partners from the University of Nottingham and BAS with the logistical support of the Australian Antarctic Division. The field sampling program was carried out from the Australian Davis station. Sampling in water bodies comprised both the collection of samples for water chemistry as well as the collection of surface sediment samples and long cores from selected water bodies. The sites were chosen to cover the entire salinity gradient of the lakes. Physical and chemical analysis of the water were carried out at the time of sampling. Field sampling in the Vestfold Hills was carried out by Johanna Laybourn-Parry, Gareth Murtagh, Paul Dyer, Ingmar Janse, Tracey Henshaw and Wendy Quayle with assistance from Davis personnel (Mark Clear, Tony Morland and others). From the Excel Spreadsheet: We isolated 59 strains of cyanobacteria from the benthic microbial mats of 23 Antarctic lakes, from 5 locations in 2 regions, in order to characterise their morphological and genotypic diversity and screen them for bioactive activities. On the basis of their morphology, the cyanobacteria were assigned to 12 species that included 4 Antarctic endemic taxa. Sequences of the ribosomal RNA gene were determined for 56 strains. In general, the strains closely related at the 16S rRNA gene level belonged to the same morphospecies. Nevertheless, divergences were found concerning the diversity in terms of species richness, novelty and geographical distribution. For 56 strains, 21 OTUs (Operational Taxonomic Unit, defined as groups of partial 16S rRNA gene sequences with more than 97.5% similarity) were found, including 9 novel and 3 exclusively Antarctic OTUs. Two sequences of Petalonema cf involvens and Chondrocystis sp. were the first to be determined for these genera. The Internally Transcribed Spacer (ITS) between the 16S and the 23S rRNA genes was sequenced for 33 strains and similar groupings were found with the 16S rRNA gene and the ITS, even when the strains were derived from different lakes and regions. After determination of the best cultivation conditions, 48 strains were grown in mass at 20 degrees C and then screened for antimicrobial and cytotoxic activities. Most strains exhibited low productivities and growth rates, similar to those reported in the literature, but were photosensitive. Seventeen strains were bioactive. The frequency of antibacterial activity against the Gram-positive Staphylococcus aureus was 29%. No activities were detected vs. the Gram-negative Escherichia coli and the yeast Candida albicans, whereas 4% and 20% of the strains inhibited the growth of Aspergillus fumigatus and Cryptococcus neoformans, respectively. Half of the strains were cytotoxic to the mammalian cell line. Given the biotechnological potential of these cyanobacterial strains, further work is in progress on the chemical characterisation of their constituent metabolites. The fields in this dataset are: Region Lake Location Latitude Longitude Strain Isolation media Number of trichomes False branching Cross-wall constriction Cross-wall granulation Necridic cell Cell shape Cell width Cell length Species Morphospecies OTU Operational Taxonomic Unit ITS Internally Transcribed Spacer Cultivation method Productivity Photosensitivity Cytotoxicity