RNA sequence data from krill CO2 exposure experiment

• Other Authors: DEAGLE, BRUCE (hasPrincipalInvestigator), DEAGLE, BRUCE (processor), Australian Antarctic Data Centre (publisher)
• Format: Dataset
• Language: unknown
• Published: Australian Antarctic Data Centre
• Subjects: biota; environment; oceans; EUPHAUSIIDS (KRILL); EARTH SCIENCE; BIOLOGICAL CLASSIFICATION; ANIMALS/INVERTEBRATES; ARTHROPODS; CRUSTACEANS; CARBON DIOXIDE; OCEAN CHEMISTRY; OCEAN ACIDIFICATION; RNA; GENETICS; ADS > Automated DNA Sequencer; LABORATORY; GEOGRAPHIC REGION > POLAR; CONTINENT > ANTARCTICA; OCEAN > SOUTHERN OCEAN; AUSTRALIA/NEW ZEALAND > AUSTRALIA; Antarctic; New Zealand; Southern Ocean; Antarc*; Antarctic Krill; Antarctica; Ocean acidification
• Online Access: https://researchdata.ands.org.au/rna-sequence-krill-exposure-experiment/698945; https://doi.org/10.4225/15/5775D60F1CB0D; https://data.aad.gov.au/metadata/records/AAS_4015_krill_RNA; http://nla.gov.au/nla.party-617536

We use RNA sequencing to investigate which genetic/physiological pathways in Antarctic krill are affected by increased CO2 levels. We carried out larval CO2 exposure experiments in March 2012 at the AAD aquarium. Two developmental stages were used (Calyptopis I and Furcilia V) and three CO2 levels (control, 1000 and 2000 ppm). These were short term experiments (2 days) - since initial longer experiments starting with fertilized eggs resulted in differences in developmental stages between treatments and control which could confound the data. RNA was extracted from larvae and high-throughput RNA sequencing (RNA-seq) was carried out on 6 samples (2 stages * 3 treatments). Sequencing was carried out on an Illumina sequencer (Genome Analyzer II). We collected ~ 60 million sequence reads per sample (Data in FASTA format each read gives 100 base pairs of sequence), so a total of ~360 million reads (36 billion bp of data).