Respiration and ammonia excretion rates of Antarctic zooplankton (Nella Dan Cruise)

• Other Authors: AIMS Data Centre (distributor), AIMS Data Centre (pointOfContact), Australian Institute of Marine Science (AIMS) (hasAssociationWith), Data Manager, AIMS Data Centre (pointOfContact), Ikeda, Tsutomu, Dr (hasPrincipalInvestigator)
• Format: Dataset
• Language: unknown
• Published: Australian Ocean Data Network
• Subjects: oceans; Antarc*; Antarctic
• Online Access: https://researchdata.edu.au/respiration-ammonia-excretion-dan-cruise/690833

Maintenance and Update Frequency: notPlanned Statement: Statement: Collection Methods:Zooplankton were collected using a 100 cm diameter, 250 cm long net, made of 1 mm mesh. Subsurface (A description of the methods used to transport live Antarctic zooplankton to the AIMS laboratory in Townsville is given in:Ikeda T, Mitchell AW, Carleton JH and Dixon P (1980) Transport of living antarctic zooplankton to a tropical laboratory: a feasibility study. Australian Journal of Marine and Freshwater Research 31: 271-274.The water bottle method used for direct measurements of respiration and ammonia excretion rates is described in:Ikeda T (1974) Nutritional ecology of marine zooplankton. Mem. Fac. Fish. Hokkaido Univ. 22, 1-97.The methods used to measure the concentration of dissolved oxygen (the Winkler method) and the ammonia concentration (the phenol-hypochlorite method) are described in: Strickland JDH, Parsons TR (1972) A practical handbook of seawater analysis. Bull. Fish Res. Board Can. 167, 1-310.Methods used in assays of ETS activity and later modifications to this method are described in:Packard TT (1971) The measurement of respiratory electron transport activity in marine phytoplankton. J. Mar. Res. 29, 235-44.Owens TG, King FD (1975) The measurement of respiratory electron-transport-system activity in marine zooplankton. Mar. Biol. 30, 27-36.Protein concentration was determined using Bovine serum albumin (Sigma, St Louis) as the standard for protein analysis. The method used for analysis is described in: Lowry OH, Rosebrough NJ, Farr AL, Randall, RJ (1951) Protein measurement with the Folin Phenol reagent. J. Biol. Chem. 193, 265-75. Credit Ikeda, Tsutomu, Dr (Principal Investigator) Zooplankton were collected from 19 stations in Antarctic waters between December 1978 and January 1979. Plankton samples were sorted into individual species and live specimens of 8 species were maintained in an incubator at -0.5 ± 0.5°C and transported to the Australian Institute of Marine Science (AIMS) for laboratory ...