RNA sequence data from krill CO2 exposure experiment

We use RNA sequencing to investigate which genetic/physiological pathways in Antarctic krill are affected by increased CO2 levels. We carried out larval CO2 exposure experiments in March 2012 at the AAD aquarium. Two developmental stages were used (Calyptopis I and Furcilia V) and three CO2 levels (...

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Bibliographic Details
Other Authors: AADC (originator), AU/AADC > Australian Antarctic Data Centre, Australia (resourceProvider)
Format: Dataset
Language:unknown
Published: Australian Ocean Data Network
Subjects:
biota
environment
oceans
EUPHAUSIIDS (KRILL)
EARTH SCIENCE
BIOLOGICAL CLASSIFICATION
ANIMALS/INVERTEBRATES
ARTHROPODS
CRUSTACEANS
CARBON DIOXIDE
OCEAN CHEMISTRY
OCEAN ACIDIFICATION
RNA
GENETICS
ADS &gt
Automated DNA Sequencer
LABORATORY
AMD/AU
AMD
CEOS
GEOGRAPHIC REGION &gt
POLAR
CONTINENT &gt
ANTARCTICA
OCEAN &gt
SOUTHERN OCEAN
AUSTRALIA/NEW ZEALAND &gt
AUSTRALIA
Antarctic
New Zealand
Southern Ocean
Antarc*
Antarctic Krill
Antarctica
Ocean acidification
Online Access:https://researchdata.ands.org.au/rna-sequence-krill-exposure-experiment/687077
https://data.aad.gov.au/metadata/records/AAS_4015_krill_RNA
https://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=AAS_4015_krill_RNA
http://data.aad.gov.au/aadc/portal/download_file.cfm?file_id=4618
https://secure3.aad.gov.au/proms/public/projects/report_project_public.cfm?project_no=AAS_4015
Description
Summary:We use RNA sequencing to investigate which genetic/physiological pathways in Antarctic krill are affected by increased CO2 levels. We carried out larval CO2 exposure experiments in March 2012 at the AAD aquarium. Two developmental stages were used (Calyptopis I and Furcilia V) and three CO2 levels (control, 1000 and 2000 ppm). These were short term experiments (2 days) - since initial longer experiments starting with fertilized eggs resulted in differences in developmental stages between treatments and control which could confound the data. RNA was extracted from larvae and high-throughput RNA sequencing (RNA-seq) was carried out on 6 samples (2 stages * 3 treatments). Sequencing was carried out on an Illumina sequencer (Genome Analyzer II). We collected ~ 60 million sequence reads per sample (Data in FASTA format each read gives 100 base pairs of sequence), so a total of ~360 million reads (36 billion bp of data).

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