Oxygen and nitrogen isotopic composition of nitrate in the Antarctic ice-edge, sea-ice and underlying seawater data collected during the SIPEX II voyage of the Aurora Australis, 2012
Each eventual parameter important for global comprehension of the dataset is highlighted as comment, when needed, in the Final Sample List.xls file (See parent record). Overview of the project and objectives: To investigate whether nitrate uptake and processes other than nitrate uptake by phytoplank...
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Format: | Dataset |
Language: | unknown |
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Australian Ocean Data Network
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Online Access: | https://researchdata.ands.org.au/oxygen-nitrogen-isotopic-australis-2012/685525 https://data.aad.gov.au/metadata/records/SIPEX_II_Stable_Isotopes_NO3 https://secure3.aad.gov.au/proms/public/projects/report_project_public.cfm?project_no=4051 https://secure3.aad.gov.au/proms/public/projects/report_project_public.cfm?project_no=4073 http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=SIPEX_II_Stable_Isotopes_NO3 |
id |
ftands:oai:ands.org.au::685525 |
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record_format |
openpolar |
institution |
Open Polar |
collection |
Research Data Australia (Australian National Data Service - ANDS) |
op_collection_id |
ftands |
language |
unknown |
topic |
oceans NITRATE EARTH SCIENCE OCEAN CHEMISTRY NITROGEN OXYGEN STABLE ISOTOPES Isotopic composition CTD > Conductivity Temperature Depth CORING DEVICES R/V AA > R/V Aurora Australis LABORATORY AMD AMD/AU CEOS ACE/CRC CONTINENT > ANTARCTICA OCEAN > SOUTHERN OCEAN GEOGRAPHIC REGION > POLAR |
spellingShingle |
oceans NITRATE EARTH SCIENCE OCEAN CHEMISTRY NITROGEN OXYGEN STABLE ISOTOPES Isotopic composition CTD > Conductivity Temperature Depth CORING DEVICES R/V AA > R/V Aurora Australis LABORATORY AMD AMD/AU CEOS ACE/CRC CONTINENT > ANTARCTICA OCEAN > SOUTHERN OCEAN GEOGRAPHIC REGION > POLAR Oxygen and nitrogen isotopic composition of nitrate in the Antarctic ice-edge, sea-ice and underlying seawater data collected during the SIPEX II voyage of the Aurora Australis, 2012 |
topic_facet |
oceans NITRATE EARTH SCIENCE OCEAN CHEMISTRY NITROGEN OXYGEN STABLE ISOTOPES Isotopic composition CTD > Conductivity Temperature Depth CORING DEVICES R/V AA > R/V Aurora Australis LABORATORY AMD AMD/AU CEOS ACE/CRC CONTINENT > ANTARCTICA OCEAN > SOUTHERN OCEAN GEOGRAPHIC REGION > POLAR |
description |
Each eventual parameter important for global comprehension of the dataset is highlighted as comment, when needed, in the Final Sample List.xls file (See parent record). Overview of the project and objectives: To investigate whether nitrate uptake and processes other than nitrate uptake by phytoplankton are significant and show spatial variability possibly induced by varying availability of Fe and other parameters in the region, seawater was collected from CTD (Conductivity, Temperature and Depth) and TMR (Trace Metals Rosette) casts jointly with the nutrient sampling, as well as well as sea-ice collected from Bio ice-core types on Ice Station, for analysis of nitrate d15N, d18O isotopic composition. Results have been interpreted in the light of prevailing nitrate-nutrient concentrations (Belgian team) and N-uptake regimes for the Ice Stations (new vs. regenerated production and nitrification; see Silicon, Carbon and Nitrogen in-situ incubation Metadata file). Methodology and sampling strategy: Samples for isotopic composition of nitrate were collected from the CTD rosette, TMR and Bio ice-core jointly with the nutrient sampling. Sea-ice sampling: sampling strategy follows ice stations deployment via Bio ice-core type. Most of the time we worked close to / directly on the Trace Metal site following precautions concerning TM sampling (clean suits etc.). When we worked close to the TM site, precautions were not such important because we don't need the same drastic precautions for our own sampling. We work together because we want to propose a set of data which helps to characterize the system of functioning in close relation with TM availability (for that, sampling location have to be as close as possible). All samples were filtered on 0.2 microns acrodiscs and kept at -20 degrees C till analysis in the home-based laboratory. We applied the denitrifier method elaborated by Sigman et al. (2001) and Casciotti et al. (2002). This method is based on the isotopic analysis of delta 15N and delta 18O of nitrous oxide (N2O) generated from nitrate by denitrifying bacteria lacking N2O-reductase activity. As a prerequisite the nitrate concentrations need to be known (nutrients analysis in the home lab.) as this sets sample amount provided to the denitrifier community. Briefly, sample nitrate is reduced by a strain of denitrifying bacteria (Pseudomonas aureofaciens) which transform nitrate into N2O, but lack the enzyme to produce N2. N2O is then analysed for N, O isotopic composition by IRMS (Delta V, Thermo) after elimination of CO2, volatile organic carbon and further cryogenic focusing of N2O (Mangion, 2011). Casciotti K.L., D.M.Sigman, M.G. Hastings, J.K. Bohlke and A. Hilkert, 2002. Measurement of the oxygen isotopic composition of nitrate in seawater and freshwater using the denitrifier method, Analytical Chemistry, 74 (19): 4905-4912. Mangion P., 2011. Biogeochemical consequences of sewage discharge on mangrove environments in East Africa, PhD Thesis, Vrije Universiteit Brussel, 208 pp. Sigman D.M., Casciotti K.L., Andreani M., Barford C., Galanter M. and J.K. Bohlke, 2001. A bacterial method for the nitrogen isotopic analysis of nitrate in seawater and freshwater, Analytical Chemistry, 73: 4145-4153. |
author2 |
AADC (originator) AU/AADC > Australian Antarctic Data Centre, Australia (resourceProvider) |
format |
Dataset |
title |
Oxygen and nitrogen isotopic composition of nitrate in the Antarctic ice-edge, sea-ice and underlying seawater data collected during the SIPEX II voyage of the Aurora Australis, 2012 |
title_short |
Oxygen and nitrogen isotopic composition of nitrate in the Antarctic ice-edge, sea-ice and underlying seawater data collected during the SIPEX II voyage of the Aurora Australis, 2012 |
title_full |
Oxygen and nitrogen isotopic composition of nitrate in the Antarctic ice-edge, sea-ice and underlying seawater data collected during the SIPEX II voyage of the Aurora Australis, 2012 |
title_fullStr |
Oxygen and nitrogen isotopic composition of nitrate in the Antarctic ice-edge, sea-ice and underlying seawater data collected during the SIPEX II voyage of the Aurora Australis, 2012 |
title_full_unstemmed |
Oxygen and nitrogen isotopic composition of nitrate in the Antarctic ice-edge, sea-ice and underlying seawater data collected during the SIPEX II voyage of the Aurora Australis, 2012 |
title_sort |
oxygen and nitrogen isotopic composition of nitrate in the antarctic ice-edge, sea-ice and underlying seawater data collected during the sipex ii voyage of the aurora australis, 2012 |
publisher |
Australian Ocean Data Network |
url |
https://researchdata.ands.org.au/oxygen-nitrogen-isotopic-australis-2012/685525 https://data.aad.gov.au/metadata/records/SIPEX_II_Stable_Isotopes_NO3 https://secure3.aad.gov.au/proms/public/projects/report_project_public.cfm?project_no=4051 https://secure3.aad.gov.au/proms/public/projects/report_project_public.cfm?project_no=4073 http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=SIPEX_II_Stable_Isotopes_NO3 |
op_coverage |
Spatial: northlimit=-62; southlimit=-67; westlimit=115; eastLimit=125 Temporal: From 2012-09-25 to 2012-11-01 |
long_lat |
ENVELOPE(-154.167,-154.167,-85.567,-85.567) ENVELOPE(115,125,-62,-67) |
geographic |
Antarctic Southern Ocean The Antarctic Hastings |
geographic_facet |
Antarctic Southern Ocean The Antarctic Hastings |
genre |
Antarc* Antarctic Antarctica aurora australis ice core Sea ice Southern Ocean |
genre_facet |
Antarc* Antarctic Antarctica aurora australis ice core Sea ice Southern Ocean |
op_source |
https://data.aad.gov.au |
op_relation |
https://researchdata.ands.org.au/oxygen-nitrogen-isotopic-australis-2012/685525 7cd43845-bdc7-49a1-ae27-9d160c5fb349 https://data.aad.gov.au/metadata/records/SIPEX_II_Stable_Isotopes_NO3 https://secure3.aad.gov.au/proms/public/projects/report_project_public.cfm?project_no=4051 https://secure3.aad.gov.au/proms/public/projects/report_project_public.cfm?project_no=4073 http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=SIPEX_II_Stable_Isotopes_NO3 |
_version_ |
1766245624321146880 |
spelling |
ftands:oai:ands.org.au::685525 2023-05-15T13:46:55+02:00 Oxygen and nitrogen isotopic composition of nitrate in the Antarctic ice-edge, sea-ice and underlying seawater data collected during the SIPEX II voyage of the Aurora Australis, 2012 AADC (originator) AU/AADC > Australian Antarctic Data Centre, Australia (resourceProvider) Spatial: northlimit=-62; southlimit=-67; westlimit=115; eastLimit=125 Temporal: From 2012-09-25 to 2012-11-01 https://researchdata.ands.org.au/oxygen-nitrogen-isotopic-australis-2012/685525 https://data.aad.gov.au/metadata/records/SIPEX_II_Stable_Isotopes_NO3 https://secure3.aad.gov.au/proms/public/projects/report_project_public.cfm?project_no=4051 https://secure3.aad.gov.au/proms/public/projects/report_project_public.cfm?project_no=4073 http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=SIPEX_II_Stable_Isotopes_NO3 unknown Australian Ocean Data Network https://researchdata.ands.org.au/oxygen-nitrogen-isotopic-australis-2012/685525 7cd43845-bdc7-49a1-ae27-9d160c5fb349 https://data.aad.gov.au/metadata/records/SIPEX_II_Stable_Isotopes_NO3 https://secure3.aad.gov.au/proms/public/projects/report_project_public.cfm?project_no=4051 https://secure3.aad.gov.au/proms/public/projects/report_project_public.cfm?project_no=4073 http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=SIPEX_II_Stable_Isotopes_NO3 https://data.aad.gov.au oceans NITRATE EARTH SCIENCE OCEAN CHEMISTRY NITROGEN OXYGEN STABLE ISOTOPES Isotopic composition CTD > Conductivity Temperature Depth CORING DEVICES R/V AA > R/V Aurora Australis LABORATORY AMD AMD/AU CEOS ACE/CRC CONTINENT > ANTARCTICA OCEAN > SOUTHERN OCEAN GEOGRAPHIC REGION > POLAR dataset ftands 2020-01-05T21:06:21Z Each eventual parameter important for global comprehension of the dataset is highlighted as comment, when needed, in the Final Sample List.xls file (See parent record). Overview of the project and objectives: To investigate whether nitrate uptake and processes other than nitrate uptake by phytoplankton are significant and show spatial variability possibly induced by varying availability of Fe and other parameters in the region, seawater was collected from CTD (Conductivity, Temperature and Depth) and TMR (Trace Metals Rosette) casts jointly with the nutrient sampling, as well as well as sea-ice collected from Bio ice-core types on Ice Station, for analysis of nitrate d15N, d18O isotopic composition. Results have been interpreted in the light of prevailing nitrate-nutrient concentrations (Belgian team) and N-uptake regimes for the Ice Stations (new vs. regenerated production and nitrification; see Silicon, Carbon and Nitrogen in-situ incubation Metadata file). Methodology and sampling strategy: Samples for isotopic composition of nitrate were collected from the CTD rosette, TMR and Bio ice-core jointly with the nutrient sampling. Sea-ice sampling: sampling strategy follows ice stations deployment via Bio ice-core type. Most of the time we worked close to / directly on the Trace Metal site following precautions concerning TM sampling (clean suits etc.). When we worked close to the TM site, precautions were not such important because we don't need the same drastic precautions for our own sampling. We work together because we want to propose a set of data which helps to characterize the system of functioning in close relation with TM availability (for that, sampling location have to be as close as possible). All samples were filtered on 0.2 microns acrodiscs and kept at -20 degrees C till analysis in the home-based laboratory. We applied the denitrifier method elaborated by Sigman et al. (2001) and Casciotti et al. (2002). This method is based on the isotopic analysis of delta 15N and delta 18O of nitrous oxide (N2O) generated from nitrate by denitrifying bacteria lacking N2O-reductase activity. As a prerequisite the nitrate concentrations need to be known (nutrients analysis in the home lab.) as this sets sample amount provided to the denitrifier community. Briefly, sample nitrate is reduced by a strain of denitrifying bacteria (Pseudomonas aureofaciens) which transform nitrate into N2O, but lack the enzyme to produce N2. N2O is then analysed for N, O isotopic composition by IRMS (Delta V, Thermo) after elimination of CO2, volatile organic carbon and further cryogenic focusing of N2O (Mangion, 2011). Casciotti K.L., D.M.Sigman, M.G. Hastings, J.K. Bohlke and A. Hilkert, 2002. Measurement of the oxygen isotopic composition of nitrate in seawater and freshwater using the denitrifier method, Analytical Chemistry, 74 (19): 4905-4912. Mangion P., 2011. Biogeochemical consequences of sewage discharge on mangrove environments in East Africa, PhD Thesis, Vrije Universiteit Brussel, 208 pp. Sigman D.M., Casciotti K.L., Andreani M., Barford C., Galanter M. and J.K. Bohlke, 2001. A bacterial method for the nitrogen isotopic analysis of nitrate in seawater and freshwater, Analytical Chemistry, 73: 4145-4153. Dataset Antarc* Antarctic Antarctica aurora australis ice core Sea ice Southern Ocean Research Data Australia (Australian National Data Service - ANDS) Antarctic Southern Ocean The Antarctic Hastings ENVELOPE(-154.167,-154.167,-85.567,-85.567) ENVELOPE(115,125,-62,-67) |