Molecular comparison of bacterial diversity in uncontaminated and hydrocarbon contaminated marine sediment

• Other Authors: AADC (originator), AU/AADC > Australian Antarctic Data Centre, Australia (resourceProvider)
• Format: Dataset
• Language: unknown
• Published: Australian Ocean Data Network
• Subjects: biota; environment; oceans; CONTAMINANT LEVELS/SPILLS; EARTH SCIENCE; HUMAN DIMENSIONS; ENVIRONMENTAL IMPACTS; OIL SPILLS; BACTERIA/ARCHAEA; BIOLOGICAL CLASSIFICATION; COMMUNITY STRUCTURE; BIOSPHERE; ECOLOGICAL DYNAMICS; COMMUNITY DYNAMICS; BACTERIAL DIVERSITY; BACTERIAL COMMUNITY STRUCTURE; DENATURING GRADIENT GEL ELECTROPHORESIS; HYDROCARBONS; NON-METRIC MULTI DIMENSIONAL SCALING PLOTS; SEDIMENT CORERS; FIELD SURVEYS; AMD/AU; CEOS; AMD; CONTINENT > ANTARCTICA; ANTARCTICA > Casey; Brown Bay; O'Brien Bay; Sparkes Bay; Newcomb Bay; GEOGRAPHIC REGION > POLAR; Antarc*; Antarctica
• Online Access: https://researchdata.ands.org.au/molecular-comparison-bacterial-marine-sediment/685320; https://data.aad.gov.au/metadata/records/Nested_DGGE; https://data.aad.gov.au/eds/2970/download; https://secure3.aad.gov.au/proms/public/projects/report_project_public.cfm?project_no=1228; http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=Nested_DGGE

Sediment samples which were originally collected as part of ASAC 868 (ASAC_868) are now being investigated using molecular microbial techniques as part of ASAC 1228 (ASAC_1228). Samples were collected in a nested survey design in two hydrocarbon impacted areas and two unimpacted areas. Denaturing gradient gel electrophoresis (DGGE) of a region of the 16S RNA gene was used to investigate the microbial community structure. Banding patterns obtained from the DGGE were transformed into a presence / absence matrix and analysed with a multivariate statistical approach. The download file contains an excel spreadsheet, a csv version of the data, plus a readme file.