BROKE-West microbial concentrations - Voyage 3 of the Aurora Australis 2005/2006
This data set contains concentrations of phytoplankton, protozoa, total bacteria and metabolically active bacteria assessed by flow cytometry on transects 12, 1, 3, 5, 7, 9 and 11 of the BROKE-West survey of the Southern Ocean between January and March 2006. Only total bacterial concentrations were...
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Australian Ocean Data Network
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Online Access: | https://researchdata.ands.org.au/broke-west-microbial-australis-20052006/684819 https://data.aad.gov.au/metadata/records/BROKE-West_flow_cytometry http://data.aad.gov.au/eds/1468/download http://data.aad.gov.au/aadc/marine_science/bottle_data/index.cfm https://secure3.aad.gov.au/proms/public/projects/report_project_public.cfm?project_no=40 http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=BROKE-West_flow_cytometry |
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ftands:oai:ands.org.au::684819 2023-05-15T13:46:55+02:00 BROKE-West microbial concentrations - Voyage 3 of the Aurora Australis 2005/2006 AADC (originator) AU/AADC > Australian Antarctic Data Centre, Australia (resourceProvider) Spatial: northlimit=-59.79757; southlimit=-69.20696; westlimit=29.99776; eastLimit=80.01055 Temporal: From 2006-01-10 to 2006-02-27 https://researchdata.ands.org.au/broke-west-microbial-australis-20052006/684819 https://data.aad.gov.au/metadata/records/BROKE-West_flow_cytometry http://data.aad.gov.au/eds/1468/download http://data.aad.gov.au/aadc/marine_science/bottle_data/index.cfm https://secure3.aad.gov.au/proms/public/projects/report_project_public.cfm?project_no=40 http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=BROKE-West_flow_cytometry unknown Australian Ocean Data Network https://researchdata.ands.org.au/broke-west-microbial-australis-20052006/684819 f9477397-0e4d-46bf-b9ef-b514e34df3d9 https://data.aad.gov.au/metadata/records/BROKE-West_flow_cytometry http://data.aad.gov.au/eds/1468/download http://data.aad.gov.au/aadc/marine_science/bottle_data/index.cfm https://secure3.aad.gov.au/proms/public/projects/report_project_public.cfm?project_no=40 http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=BROKE-West_flow_cytometry https://data.aad.gov.au biota oceans PROTISTS EARTH SCIENCE BIOLOGICAL CLASSIFICATION BACTERIA/ARCHAEA PHYTOPLANKTON BIOSPHERE AQUATIC ECOSYSTEMS PLANKTON EARTH SCIENCE > BIOSPHERE > ECOSYSTEMS > MARINE ECOSYSTEMS > PELAGIC BROKE-West Flow cytometry bacteria FACScan Flow Cytometer Latitude Longitude Transect Number CTD Depth Total bacteria Active bacteria Dead bacteria Protozoa SHIPS R/V AA > R/V Aurora Australis AMD/AU CEOS AMD ACE/CRC OCEAN > SOUTHERN OCEAN CONTINENT > ANTARCTICA GEOGRAPHIC REGION > POLAR dataset ftands 2020-01-05T21:05:40Z This data set contains concentrations of phytoplankton, protozoa, total bacteria and metabolically active bacteria assessed by flow cytometry on transects 12, 1, 3, 5, 7, 9 and 11 of the BROKE-West survey of the Southern Ocean between January and March 2006. Only total bacterial concentrations were assessed for transect 11. Between 4 and 12 depths were sampled for marine microbes and concentrations were assessed using FACScan flowcytometer. Phytoplankton were identified and counted based on the autofluorescense of chlorophyll a when excited by the 488 nm laser of the FACScan. Protozoa were identified and counted after staining with the acid vacuole stain Lysotracker Green. Total bacteria were identified and counted using the cell permeant SYTO 13 nucleic stain. Metabolically active bacteria were identified and counted after staining for intracellular esterases with the esterase stain 6CFDA. The fields in this dataset are: Latitude Longitude Transect Number CTD number, flow file Depth (m) Total bacteria (per millilitre) Active bacteria (per millilitre) Dead bacteria (per millilitre) Protozoa (per millilitre) Phytoplankton (per millilitre) This work was completed as part of ASAC project 40 (ASAC_40). Dataset Antarc* Antarctica aurora australis Southern Ocean Research Data Australia (Australian National Data Service - ANDS) Southern Ocean ENVELOPE(29.99776,80.01055,-59.79757,-69.20696) |
institution |
Open Polar |
collection |
Research Data Australia (Australian National Data Service - ANDS) |
op_collection_id |
ftands |
language |
unknown |
topic |
biota oceans PROTISTS EARTH SCIENCE BIOLOGICAL CLASSIFICATION BACTERIA/ARCHAEA PHYTOPLANKTON BIOSPHERE AQUATIC ECOSYSTEMS PLANKTON EARTH SCIENCE > BIOSPHERE > ECOSYSTEMS > MARINE ECOSYSTEMS > PELAGIC BROKE-West Flow cytometry bacteria FACScan Flow Cytometer Latitude Longitude Transect Number CTD Depth Total bacteria Active bacteria Dead bacteria Protozoa SHIPS R/V AA > R/V Aurora Australis AMD/AU CEOS AMD ACE/CRC OCEAN > SOUTHERN OCEAN CONTINENT > ANTARCTICA GEOGRAPHIC REGION > POLAR |
spellingShingle |
biota oceans PROTISTS EARTH SCIENCE BIOLOGICAL CLASSIFICATION BACTERIA/ARCHAEA PHYTOPLANKTON BIOSPHERE AQUATIC ECOSYSTEMS PLANKTON EARTH SCIENCE > BIOSPHERE > ECOSYSTEMS > MARINE ECOSYSTEMS > PELAGIC BROKE-West Flow cytometry bacteria FACScan Flow Cytometer Latitude Longitude Transect Number CTD Depth Total bacteria Active bacteria Dead bacteria Protozoa SHIPS R/V AA > R/V Aurora Australis AMD/AU CEOS AMD ACE/CRC OCEAN > SOUTHERN OCEAN CONTINENT > ANTARCTICA GEOGRAPHIC REGION > POLAR BROKE-West microbial concentrations - Voyage 3 of the Aurora Australis 2005/2006 |
topic_facet |
biota oceans PROTISTS EARTH SCIENCE BIOLOGICAL CLASSIFICATION BACTERIA/ARCHAEA PHYTOPLANKTON BIOSPHERE AQUATIC ECOSYSTEMS PLANKTON EARTH SCIENCE > BIOSPHERE > ECOSYSTEMS > MARINE ECOSYSTEMS > PELAGIC BROKE-West Flow cytometry bacteria FACScan Flow Cytometer Latitude Longitude Transect Number CTD Depth Total bacteria Active bacteria Dead bacteria Protozoa SHIPS R/V AA > R/V Aurora Australis AMD/AU CEOS AMD ACE/CRC OCEAN > SOUTHERN OCEAN CONTINENT > ANTARCTICA GEOGRAPHIC REGION > POLAR |
description |
This data set contains concentrations of phytoplankton, protozoa, total bacteria and metabolically active bacteria assessed by flow cytometry on transects 12, 1, 3, 5, 7, 9 and 11 of the BROKE-West survey of the Southern Ocean between January and March 2006. Only total bacterial concentrations were assessed for transect 11. Between 4 and 12 depths were sampled for marine microbes and concentrations were assessed using FACScan flowcytometer. Phytoplankton were identified and counted based on the autofluorescense of chlorophyll a when excited by the 488 nm laser of the FACScan. Protozoa were identified and counted after staining with the acid vacuole stain Lysotracker Green. Total bacteria were identified and counted using the cell permeant SYTO 13 nucleic stain. Metabolically active bacteria were identified and counted after staining for intracellular esterases with the esterase stain 6CFDA. The fields in this dataset are: Latitude Longitude Transect Number CTD number, flow file Depth (m) Total bacteria (per millilitre) Active bacteria (per millilitre) Dead bacteria (per millilitre) Protozoa (per millilitre) Phytoplankton (per millilitre) This work was completed as part of ASAC project 40 (ASAC_40). |
author2 |
AADC (originator) AU/AADC > Australian Antarctic Data Centre, Australia (resourceProvider) |
format |
Dataset |
title |
BROKE-West microbial concentrations - Voyage 3 of the Aurora Australis 2005/2006 |
title_short |
BROKE-West microbial concentrations - Voyage 3 of the Aurora Australis 2005/2006 |
title_full |
BROKE-West microbial concentrations - Voyage 3 of the Aurora Australis 2005/2006 |
title_fullStr |
BROKE-West microbial concentrations - Voyage 3 of the Aurora Australis 2005/2006 |
title_full_unstemmed |
BROKE-West microbial concentrations - Voyage 3 of the Aurora Australis 2005/2006 |
title_sort |
broke-west microbial concentrations - voyage 3 of the aurora australis 2005/2006 |
publisher |
Australian Ocean Data Network |
url |
https://researchdata.ands.org.au/broke-west-microbial-australis-20052006/684819 https://data.aad.gov.au/metadata/records/BROKE-West_flow_cytometry http://data.aad.gov.au/eds/1468/download http://data.aad.gov.au/aadc/marine_science/bottle_data/index.cfm https://secure3.aad.gov.au/proms/public/projects/report_project_public.cfm?project_no=40 http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=BROKE-West_flow_cytometry |
op_coverage |
Spatial: northlimit=-59.79757; southlimit=-69.20696; westlimit=29.99776; eastLimit=80.01055 Temporal: From 2006-01-10 to 2006-02-27 |
long_lat |
ENVELOPE(29.99776,80.01055,-59.79757,-69.20696) |
geographic |
Southern Ocean |
geographic_facet |
Southern Ocean |
genre |
Antarc* Antarctica aurora australis Southern Ocean |
genre_facet |
Antarc* Antarctica aurora australis Southern Ocean |
op_source |
https://data.aad.gov.au |
op_relation |
https://researchdata.ands.org.au/broke-west-microbial-australis-20052006/684819 f9477397-0e4d-46bf-b9ef-b514e34df3d9 https://data.aad.gov.au/metadata/records/BROKE-West_flow_cytometry http://data.aad.gov.au/eds/1468/download http://data.aad.gov.au/aadc/marine_science/bottle_data/index.cfm https://secure3.aad.gov.au/proms/public/projects/report_project_public.cfm?project_no=40 http://data.aad.gov.au/aadc/metadata/citation.cfm?entry_id=BROKE-West_flow_cytometry |
_version_ |
1766245573551194112 |