Southern Surveyor Voyage SS 07/2005 HPLC Pigment and Ocean Colour Data
Progress Code: completed Maintenance and Update Frequency: notPlanned Statement: Water samples were taken on-board the vessel and stored under cool and dark conditions until filtering took place on land. Samples were analysed and QC procedures were carried out in the Ocean Colour Laboratory. Pigment...
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Australian Ocean Data Network
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Online Access: | https://researchdata.edu.au/southern-surveyor-voyage-colour-data/683798 |
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ftands:oai:ands.org.au::683798 2024-09-15T18:37:27+00:00 Southern Surveyor Voyage SS 07/2005 HPLC Pigment and Ocean Colour Data CSIRO O&A, Information & Data Centre (pointOfContact) CSIRO Oceans & Atmosphere - Aspendale (hasAssociationWith) CSIRO Oceans & Atmosphere - Hobart (hasAssociationWith) CSIRO Oceans & Atmosphere - IOMRC Crawley (hasAssociationWith) CSIRO Oceans & Atmosphere - St. Lucia (hasAssociationWith) Clementson, Lesley (pointOfContact) Spatial: westlimit=112.251; southlimit=-35.436; eastlimit=118.659; northlimit=-21.008 Spatial: uplimit=0; downlimit=-204 Temporal: From 2005-07-21 to 2005-08-15 https://researchdata.edu.au/southern-surveyor-voyage-colour-data/683798 unknown Australian Ocean Data Network https://researchdata.edu.au/southern-surveyor-voyage-colour-data/683798 f2e138d2-2c51-452c-e043-08114f8cc8ca Marlin Record Number: 14370 Anzlic Identifier: ANZCW0306014370 CSIRO Oceans & Atmosphere - Hobart oceans Earth Science | Oceans | Ocean Chemistry | Chlorophyll Earth Science | Oceans | Ocean Chemistry | Pigments Earth Science | Oceans | Ocean Optics | Ocean Color Earth Science | Terrestrial Hydrosphere | Water Quality/Water Chemistry | Chlorophyll CTDs (Conductivity-Temperature-Depth Profilers) Fluorometers HPLC (High Performance Liquid Chromatography) Niskin Bottles Spectrophotometers Coastal Waters (Australia) | West Australia Coast WA Global / Oceans | Southern Ocean WfO Biogeochemistry Research Voyage: SS 07/2005 WFO - Characterisation and inventory of benthic ecosystems of the South Western Region using optimised mapping techniques Ship: Southern Surveyor Practical salinity of the water body Temperature of the water body Pressure (measured variable) in the water body exerted by overlying sea water and any medium above it Pressure (measured variable) in the water body exerted by overlying sea water only CTD research vessel dataset ftands 2024-08-06T01:59:00Z Progress Code: completed Maintenance and Update Frequency: notPlanned Statement: Water samples were taken on-board the vessel and stored under cool and dark conditions until filtering took place on land. Samples were analysed and QC procedures were carried out in the Ocean Colour Laboratory. Pigment analysis 4 litres of sample water was filtered through a 47 mm glass fibre filter (Whatman GF/F) and then stored in liquid nitrogen until analysis. To extract the pigments, the filters were cut into small pieces and covered with 100% acetone (3 mls) in a 10 ml centrifuge tube. The samples were vortexed for about 30 seconds and then sonicated for 15 minutes in the dark. The samples were then kept in the dark at 4 °C for approximately 15 hours. After this time 200 µL water was added to the acetone such that the extract mixture was 90:10 acetone:water (vol:vol) and sonicated once more for 15 minutes. The extracts were centrifuged to remove the filter paper and then filtered through a 0.2 µm membrane filter (Whatman, anatope) prior to analysis by HPLC using a Waters Alliance high performance liquid chromatography system, comprising a 2695XE separations module with column heater and refrigerated autosampler and a 2996 photo-diode array detector. Immediately prior to injection the sample extract was mixed with a buffer solution (90:10 28 mM tetrabutyl ammonium acetate, pH 6.5 : methanol) within the sample loop. Pigments were separated using a Zorbax Eclipse XDB-C8 stainless steel 150 mm x 4.6 mm ID column with 3.5 µm particle size (Agilent Technologies) with gradient elution as described in Van Heukelem and Thomas (2001). The separated pigments were detected at 436 nm and identified against standard spectra using Waters Empower software. Concentrations of pigments were determined from standards (Sigma, USA or DHI, Denmark). Spectral absorption 4 litres of sample water was filtered through a 25 mm glass fibre filter (Whatman GF/F) and the filter then stored flat in liquid nitrogen until analysis. Credit Lesley ... Dataset Southern Ocean Research Data Australia (Australian National Data Service - ANDS) |
institution |
Open Polar |
collection |
Research Data Australia (Australian National Data Service - ANDS) |
op_collection_id |
ftands |
language |
unknown |
topic |
oceans Earth Science | Oceans | Ocean Chemistry | Chlorophyll Earth Science | Oceans | Ocean Chemistry | Pigments Earth Science | Oceans | Ocean Optics | Ocean Color Earth Science | Terrestrial Hydrosphere | Water Quality/Water Chemistry | Chlorophyll CTDs (Conductivity-Temperature-Depth Profilers) Fluorometers HPLC (High Performance Liquid Chromatography) Niskin Bottles Spectrophotometers Coastal Waters (Australia) | West Australia Coast WA Global / Oceans | Southern Ocean WfO Biogeochemistry Research Voyage: SS 07/2005 WFO - Characterisation and inventory of benthic ecosystems of the South Western Region using optimised mapping techniques Ship: Southern Surveyor Practical salinity of the water body Temperature of the water body Pressure (measured variable) in the water body exerted by overlying sea water and any medium above it Pressure (measured variable) in the water body exerted by overlying sea water only CTD research vessel |
spellingShingle |
oceans Earth Science | Oceans | Ocean Chemistry | Chlorophyll Earth Science | Oceans | Ocean Chemistry | Pigments Earth Science | Oceans | Ocean Optics | Ocean Color Earth Science | Terrestrial Hydrosphere | Water Quality/Water Chemistry | Chlorophyll CTDs (Conductivity-Temperature-Depth Profilers) Fluorometers HPLC (High Performance Liquid Chromatography) Niskin Bottles Spectrophotometers Coastal Waters (Australia) | West Australia Coast WA Global / Oceans | Southern Ocean WfO Biogeochemistry Research Voyage: SS 07/2005 WFO - Characterisation and inventory of benthic ecosystems of the South Western Region using optimised mapping techniques Ship: Southern Surveyor Practical salinity of the water body Temperature of the water body Pressure (measured variable) in the water body exerted by overlying sea water and any medium above it Pressure (measured variable) in the water body exerted by overlying sea water only CTD research vessel Southern Surveyor Voyage SS 07/2005 HPLC Pigment and Ocean Colour Data |
topic_facet |
oceans Earth Science | Oceans | Ocean Chemistry | Chlorophyll Earth Science | Oceans | Ocean Chemistry | Pigments Earth Science | Oceans | Ocean Optics | Ocean Color Earth Science | Terrestrial Hydrosphere | Water Quality/Water Chemistry | Chlorophyll CTDs (Conductivity-Temperature-Depth Profilers) Fluorometers HPLC (High Performance Liquid Chromatography) Niskin Bottles Spectrophotometers Coastal Waters (Australia) | West Australia Coast WA Global / Oceans | Southern Ocean WfO Biogeochemistry Research Voyage: SS 07/2005 WFO - Characterisation and inventory of benthic ecosystems of the South Western Region using optimised mapping techniques Ship: Southern Surveyor Practical salinity of the water body Temperature of the water body Pressure (measured variable) in the water body exerted by overlying sea water and any medium above it Pressure (measured variable) in the water body exerted by overlying sea water only CTD research vessel |
description |
Progress Code: completed Maintenance and Update Frequency: notPlanned Statement: Water samples were taken on-board the vessel and stored under cool and dark conditions until filtering took place on land. Samples were analysed and QC procedures were carried out in the Ocean Colour Laboratory. Pigment analysis 4 litres of sample water was filtered through a 47 mm glass fibre filter (Whatman GF/F) and then stored in liquid nitrogen until analysis. To extract the pigments, the filters were cut into small pieces and covered with 100% acetone (3 mls) in a 10 ml centrifuge tube. The samples were vortexed for about 30 seconds and then sonicated for 15 minutes in the dark. The samples were then kept in the dark at 4 °C for approximately 15 hours. After this time 200 µL water was added to the acetone such that the extract mixture was 90:10 acetone:water (vol:vol) and sonicated once more for 15 minutes. The extracts were centrifuged to remove the filter paper and then filtered through a 0.2 µm membrane filter (Whatman, anatope) prior to analysis by HPLC using a Waters Alliance high performance liquid chromatography system, comprising a 2695XE separations module with column heater and refrigerated autosampler and a 2996 photo-diode array detector. Immediately prior to injection the sample extract was mixed with a buffer solution (90:10 28 mM tetrabutyl ammonium acetate, pH 6.5 : methanol) within the sample loop. Pigments were separated using a Zorbax Eclipse XDB-C8 stainless steel 150 mm x 4.6 mm ID column with 3.5 µm particle size (Agilent Technologies) with gradient elution as described in Van Heukelem and Thomas (2001). The separated pigments were detected at 436 nm and identified against standard spectra using Waters Empower software. Concentrations of pigments were determined from standards (Sigma, USA or DHI, Denmark). Spectral absorption 4 litres of sample water was filtered through a 25 mm glass fibre filter (Whatman GF/F) and the filter then stored flat in liquid nitrogen until analysis. Credit Lesley ... |
author2 |
CSIRO O&A, Information & Data Centre (pointOfContact) CSIRO Oceans & Atmosphere - Aspendale (hasAssociationWith) CSIRO Oceans & Atmosphere - Hobart (hasAssociationWith) CSIRO Oceans & Atmosphere - IOMRC Crawley (hasAssociationWith) CSIRO Oceans & Atmosphere - St. Lucia (hasAssociationWith) Clementson, Lesley (pointOfContact) |
format |
Dataset |
title |
Southern Surveyor Voyage SS 07/2005 HPLC Pigment and Ocean Colour Data |
title_short |
Southern Surveyor Voyage SS 07/2005 HPLC Pigment and Ocean Colour Data |
title_full |
Southern Surveyor Voyage SS 07/2005 HPLC Pigment and Ocean Colour Data |
title_fullStr |
Southern Surveyor Voyage SS 07/2005 HPLC Pigment and Ocean Colour Data |
title_full_unstemmed |
Southern Surveyor Voyage SS 07/2005 HPLC Pigment and Ocean Colour Data |
title_sort |
southern surveyor voyage ss 07/2005 hplc pigment and ocean colour data |
publisher |
Australian Ocean Data Network |
url |
https://researchdata.edu.au/southern-surveyor-voyage-colour-data/683798 |
op_coverage |
Spatial: westlimit=112.251; southlimit=-35.436; eastlimit=118.659; northlimit=-21.008 Spatial: uplimit=0; downlimit=-204 Temporal: From 2005-07-21 to 2005-08-15 |
genre |
Southern Ocean |
genre_facet |
Southern Ocean |
op_source |
CSIRO Oceans & Atmosphere - Hobart |
op_relation |
https://researchdata.edu.au/southern-surveyor-voyage-colour-data/683798 f2e138d2-2c51-452c-e043-08114f8cc8ca Marlin Record Number: 14370 Anzlic Identifier: ANZCW0306014370 |
_version_ |
1810481834464641024 |