Marine eukaryotes and bacteria, Antarctica
Maintenance and Update Frequency: notPlanned Credit Webster, Nicole S, Dr (Principal Investigator) Glass slides were deployed at 12m and 18m3 at sites in McMurdo Sound (adjacent to McMurdo Station, Scott Base and Cape Armitage) for a period of 12 months to collect biofilm.18S rDNA sequence informati...
Other Authors: | , , , , |
---|---|
Format: | Dataset |
Language: | unknown |
Published: |
Australian Ocean Data Network
|
Subjects: | |
Online Access: | https://researchdata.edu.au/marine-eukaryotes-bacteria-antarctica/680834 |
id |
ftands:oai:ands.org.au::680834 |
---|---|
record_format |
openpolar |
spelling |
ftands:oai:ands.org.au::680834 2024-09-15T17:43:47+00:00 Marine eukaryotes and bacteria, Antarctica AIMS Data Centre (distributor) AIMS Data Centre (pointOfContact) Australian Institute of Marine Science (AIMS) (hasAssociationWith) Data Manager, AIMS Data Centre (pointOfContact) Webster, Nicole S, Dr (hasPrincipalInvestigator) Spatial: westlimit=166.6732; southlimit=-77.863; eastlimit=166.6732; northlimit=-77.863 Spatial: westlimit=166.75; southlimit=-77.855; eastlimit=166.75; northlimit=-77.855 Spatial: westlimit=166.652; southlimit=-77.857; eastlimit=166.652; northlimit=-77.857 https://researchdata.edu.au/marine-eukaryotes-bacteria-antarctica/680834 unknown Australian Ocean Data Network https://researchdata.edu.au/marine-eukaryotes-bacteria-antarctica/680834 db3eae60-19b1-48bd-96a2-22bf4dd3ce47 Australian Institute of Marine Science (AIMS) oceans dataset ftands 2024-08-19T23:59:40Z Maintenance and Update Frequency: notPlanned Credit Webster, Nicole S, Dr (Principal Investigator) Glass slides were deployed at 12m and 18m3 at sites in McMurdo Sound (adjacent to McMurdo Station, Scott Base and Cape Armitage) for a period of 12 months to collect biofilm.18S rDNA sequence information was obtained for representatives of each DGGE (denaturing gradient gel electrophoresis) band at each site and depth. This data was interrogated to phyla level: cnidaria, bryozoa, protozoa, dinoflagellates, arthropods, platyhelminths and annelids were present.Density of diatoms observed by fluorescence microscopy for each site and depth were recorded (per 100 µm2). At least 6 different diatom morphotypes were identified based on size and morphology.The slides were also examined by performing fluorescence in situ hybridization (FISH) reactions with bacteria-specific probes (Eub338), an archaea-specific probe (ARCH915) and 9 different bacterial group-specific probes. Bacterial groups identified from the sequences were: Gammaproteobacteria, SRB, Planctomycetales, Alphaproteobacteria, Archaea, Betaproteobacteria, Actinobacteria and Firmicutes, Cytophaga /Flavobacteria of Bacteroidetes (CFB), Verrucomicrobia.This study was the first to examine eukaryotic recruitment, potentially overlooked in visual surveys, using molecular techniques. To use molecular techniques to examine the community structure of marine eukaryotes and bacteria recruiting onto artificial substrata. Dataset Antarc* Antarctica McMurdo Sound Research Data Australia (Australian National Data Service - ANDS) |
institution |
Open Polar |
collection |
Research Data Australia (Australian National Data Service - ANDS) |
op_collection_id |
ftands |
language |
unknown |
topic |
oceans |
spellingShingle |
oceans Marine eukaryotes and bacteria, Antarctica |
topic_facet |
oceans |
description |
Maintenance and Update Frequency: notPlanned Credit Webster, Nicole S, Dr (Principal Investigator) Glass slides were deployed at 12m and 18m3 at sites in McMurdo Sound (adjacent to McMurdo Station, Scott Base and Cape Armitage) for a period of 12 months to collect biofilm.18S rDNA sequence information was obtained for representatives of each DGGE (denaturing gradient gel electrophoresis) band at each site and depth. This data was interrogated to phyla level: cnidaria, bryozoa, protozoa, dinoflagellates, arthropods, platyhelminths and annelids were present.Density of diatoms observed by fluorescence microscopy for each site and depth were recorded (per 100 µm2). At least 6 different diatom morphotypes were identified based on size and morphology.The slides were also examined by performing fluorescence in situ hybridization (FISH) reactions with bacteria-specific probes (Eub338), an archaea-specific probe (ARCH915) and 9 different bacterial group-specific probes. Bacterial groups identified from the sequences were: Gammaproteobacteria, SRB, Planctomycetales, Alphaproteobacteria, Archaea, Betaproteobacteria, Actinobacteria and Firmicutes, Cytophaga /Flavobacteria of Bacteroidetes (CFB), Verrucomicrobia.This study was the first to examine eukaryotic recruitment, potentially overlooked in visual surveys, using molecular techniques. To use molecular techniques to examine the community structure of marine eukaryotes and bacteria recruiting onto artificial substrata. |
author2 |
AIMS Data Centre (distributor) AIMS Data Centre (pointOfContact) Australian Institute of Marine Science (AIMS) (hasAssociationWith) Data Manager, AIMS Data Centre (pointOfContact) Webster, Nicole S, Dr (hasPrincipalInvestigator) |
format |
Dataset |
title |
Marine eukaryotes and bacteria, Antarctica |
title_short |
Marine eukaryotes and bacteria, Antarctica |
title_full |
Marine eukaryotes and bacteria, Antarctica |
title_fullStr |
Marine eukaryotes and bacteria, Antarctica |
title_full_unstemmed |
Marine eukaryotes and bacteria, Antarctica |
title_sort |
marine eukaryotes and bacteria, antarctica |
publisher |
Australian Ocean Data Network |
url |
https://researchdata.edu.au/marine-eukaryotes-bacteria-antarctica/680834 |
op_coverage |
Spatial: westlimit=166.6732; southlimit=-77.863; eastlimit=166.6732; northlimit=-77.863 Spatial: westlimit=166.75; southlimit=-77.855; eastlimit=166.75; northlimit=-77.855 Spatial: westlimit=166.652; southlimit=-77.857; eastlimit=166.652; northlimit=-77.857 |
genre |
Antarc* Antarctica McMurdo Sound |
genre_facet |
Antarc* Antarctica McMurdo Sound |
op_source |
Australian Institute of Marine Science (AIMS) |
op_relation |
https://researchdata.edu.au/marine-eukaryotes-bacteria-antarctica/680834 db3eae60-19b1-48bd-96a2-22bf4dd3ce47 |
_version_ |
1810490964697939968 |