Southern Surveyor Voyage SS 11/95 HPLC Pigment and Ocean Colour Data
Progress Code: completed Maintenance and Update Frequency: notPlanned Statement: Water samples were taken on-board the RV Southern Surveyor. Samples were filtered onboard. Samples were analysed and QC procedures were carried out in the Ocean Colour Laboratory. Pigment analysis 4 litres of sample wat...
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Integrated Marine Observing System
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Online Access: | https://researchdata.edu.au/southern-surveyor-voyage-colour-data/476170 |
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ftands:oai:ands.org.au::476170 2024-09-15T18:37:27+00:00 Southern Surveyor Voyage SS 11/95 HPLC Pigment and Ocean Colour Data CSIRO O&A, Information & Data Centre (pointOfContact) CSIRO Oceans & Atmosphere - Aspendale (hasAssociationWith) CSIRO Oceans & Atmosphere - Hobart (hasAssociationWith) CSIRO Oceans & Atmosphere - IOMRC Crawley (hasAssociationWith) CSIRO Oceans & Atmosphere - St. Lucia (hasAssociationWith) Clementson, Lesley (pointOfContact) Spatial: westlimit=139; southlimit=-53; eastlimit=152; northlimit=-40 Temporal: From 1995-11-15 to 1995-12-07 https://researchdata.edu.au/southern-surveyor-voyage-colour-data/476170 unknown Integrated Marine Observing System https://researchdata.edu.au/southern-surveyor-voyage-colour-data/476170 a2683507-5abd-402b-a95e-8e42c47bcd7c Marlin Record Number: 6444 Anzlic Identifier: ANZCW0306006444 CSIRO Oceans & Atmosphere - Hobart oceans Earth Science | Biological Classification | Protists | Plankton | Phytoplankton Earth Science | Oceans | Ocean Chemistry | Pigments Earth Science | Oceans | Ocean Optics | Absorption Earth Science | Oceans | Ocean Optics | Fluorescence Earth Science | Oceans | Ocean Optics | Gelbstoff Earth Science | Oceans | Ocean Optics | Ocean Color Earth Science | Terrestrial Hydrosphere | Water Quality/Water Chemistry | Chlorophyll CTDs (Conductivity-Temperature-Depth Profilers) Fluorometers HPLC (High Performance Liquid Chromatography) Niskin Bottles Spectrophotometers Global / Oceans | Southern Ocean States Territories (Australia) | Tasmania WfO Biogeochemistry Research Voyage: SS 11/95 Remote sensing of ocean colour - JGFOS 1996-1999 Ship: Southern Surveyor Practical salinity of the water body Temperature of the water body Pressure (measured variable) in the water body exerted by overlying sea water and any medium above it Pressure (measured variable) in the water body exerted by overlying sea water only CTD research vessel dataset ftands 2024-08-06T01:58:59Z Progress Code: completed Maintenance and Update Frequency: notPlanned Statement: Water samples were taken on-board the RV Southern Surveyor. Samples were filtered onboard. Samples were analysed and QC procedures were carried out in the Ocean Colour Laboratory. Pigment analysis 4 litres of sample water was filtered through a 47 mm glass fibre filter (Whatman GF/F) and then stored in liquid nitrogen until analysis. To extract the pigments, the filters were cut into small pieces and covered with 100% acetone (3 mls) in a 10 ml centrifuge tube. The samples were vortexed for about 30 seconds and then sonicated for 15 minutes in the dark. The samples were then kept in the dark at 4 °C for approximately 15 hours. After this time 200 µL water was added to the acetone such that the extract mixture was 90:10 acetone:water (vol:vol) and sonicated once more for 15 minutes. The extracts were centrifuged to remove the filter paper and then filtered through a 0.2 µm membrane filter (Whatman, anatope) prior to analysis by HPLC using a Waters high performance liquid chromatograph, comprising a 600 controller, 717 plus refrigerated autosampler and a 996 photo-diode array detector. Pigments were separated using a stainless steel 25 cm x 4.6 mm I.D. column packed with ODS2 of 5 µm particle size (SGE) with gradient elution as described in Wright et al., [1991]. The separated pigments were detected at 436 nm and identified against standard spectra using Waters Millenium software. Concentrations of chlorophyll a, chlorophyll b, b,b-carotene and b,e-carotene in sample chromatograms were determined from standards (Sigma) and all other pigment concentrations were determined from standards of purified pigments isolated from algal cultures. Spectral absorption. 2 litres of sample water was filtered through a 25 mm glass fibre filter (Whatman GF/F) and the filter then stored flat in liquid nitrogen until analysis. Optical density spectra for total particulate matter were obtained using a GBC 916 UV/VIS dual beam spectrophotomete ... Dataset Southern Ocean Research Data Australia (Australian National Data Service - ANDS) |
institution |
Open Polar |
collection |
Research Data Australia (Australian National Data Service - ANDS) |
op_collection_id |
ftands |
language |
unknown |
topic |
oceans Earth Science | Biological Classification | Protists | Plankton | Phytoplankton Earth Science | Oceans | Ocean Chemistry | Pigments Earth Science | Oceans | Ocean Optics | Absorption Earth Science | Oceans | Ocean Optics | Fluorescence Earth Science | Oceans | Ocean Optics | Gelbstoff Earth Science | Oceans | Ocean Optics | Ocean Color Earth Science | Terrestrial Hydrosphere | Water Quality/Water Chemistry | Chlorophyll CTDs (Conductivity-Temperature-Depth Profilers) Fluorometers HPLC (High Performance Liquid Chromatography) Niskin Bottles Spectrophotometers Global / Oceans | Southern Ocean States Territories (Australia) | Tasmania WfO Biogeochemistry Research Voyage: SS 11/95 Remote sensing of ocean colour - JGFOS 1996-1999 Ship: Southern Surveyor Practical salinity of the water body Temperature of the water body Pressure (measured variable) in the water body exerted by overlying sea water and any medium above it Pressure (measured variable) in the water body exerted by overlying sea water only CTD research vessel |
spellingShingle |
oceans Earth Science | Biological Classification | Protists | Plankton | Phytoplankton Earth Science | Oceans | Ocean Chemistry | Pigments Earth Science | Oceans | Ocean Optics | Absorption Earth Science | Oceans | Ocean Optics | Fluorescence Earth Science | Oceans | Ocean Optics | Gelbstoff Earth Science | Oceans | Ocean Optics | Ocean Color Earth Science | Terrestrial Hydrosphere | Water Quality/Water Chemistry | Chlorophyll CTDs (Conductivity-Temperature-Depth Profilers) Fluorometers HPLC (High Performance Liquid Chromatography) Niskin Bottles Spectrophotometers Global / Oceans | Southern Ocean States Territories (Australia) | Tasmania WfO Biogeochemistry Research Voyage: SS 11/95 Remote sensing of ocean colour - JGFOS 1996-1999 Ship: Southern Surveyor Practical salinity of the water body Temperature of the water body Pressure (measured variable) in the water body exerted by overlying sea water and any medium above it Pressure (measured variable) in the water body exerted by overlying sea water only CTD research vessel Southern Surveyor Voyage SS 11/95 HPLC Pigment and Ocean Colour Data |
topic_facet |
oceans Earth Science | Biological Classification | Protists | Plankton | Phytoplankton Earth Science | Oceans | Ocean Chemistry | Pigments Earth Science | Oceans | Ocean Optics | Absorption Earth Science | Oceans | Ocean Optics | Fluorescence Earth Science | Oceans | Ocean Optics | Gelbstoff Earth Science | Oceans | Ocean Optics | Ocean Color Earth Science | Terrestrial Hydrosphere | Water Quality/Water Chemistry | Chlorophyll CTDs (Conductivity-Temperature-Depth Profilers) Fluorometers HPLC (High Performance Liquid Chromatography) Niskin Bottles Spectrophotometers Global / Oceans | Southern Ocean States Territories (Australia) | Tasmania WfO Biogeochemistry Research Voyage: SS 11/95 Remote sensing of ocean colour - JGFOS 1996-1999 Ship: Southern Surveyor Practical salinity of the water body Temperature of the water body Pressure (measured variable) in the water body exerted by overlying sea water and any medium above it Pressure (measured variable) in the water body exerted by overlying sea water only CTD research vessel |
description |
Progress Code: completed Maintenance and Update Frequency: notPlanned Statement: Water samples were taken on-board the RV Southern Surveyor. Samples were filtered onboard. Samples were analysed and QC procedures were carried out in the Ocean Colour Laboratory. Pigment analysis 4 litres of sample water was filtered through a 47 mm glass fibre filter (Whatman GF/F) and then stored in liquid nitrogen until analysis. To extract the pigments, the filters were cut into small pieces and covered with 100% acetone (3 mls) in a 10 ml centrifuge tube. The samples were vortexed for about 30 seconds and then sonicated for 15 minutes in the dark. The samples were then kept in the dark at 4 °C for approximately 15 hours. After this time 200 µL water was added to the acetone such that the extract mixture was 90:10 acetone:water (vol:vol) and sonicated once more for 15 minutes. The extracts were centrifuged to remove the filter paper and then filtered through a 0.2 µm membrane filter (Whatman, anatope) prior to analysis by HPLC using a Waters high performance liquid chromatograph, comprising a 600 controller, 717 plus refrigerated autosampler and a 996 photo-diode array detector. Pigments were separated using a stainless steel 25 cm x 4.6 mm I.D. column packed with ODS2 of 5 µm particle size (SGE) with gradient elution as described in Wright et al., [1991]. The separated pigments were detected at 436 nm and identified against standard spectra using Waters Millenium software. Concentrations of chlorophyll a, chlorophyll b, b,b-carotene and b,e-carotene in sample chromatograms were determined from standards (Sigma) and all other pigment concentrations were determined from standards of purified pigments isolated from algal cultures. Spectral absorption. 2 litres of sample water was filtered through a 25 mm glass fibre filter (Whatman GF/F) and the filter then stored flat in liquid nitrogen until analysis. Optical density spectra for total particulate matter were obtained using a GBC 916 UV/VIS dual beam spectrophotomete ... |
author2 |
CSIRO O&A, Information & Data Centre (pointOfContact) CSIRO Oceans & Atmosphere - Aspendale (hasAssociationWith) CSIRO Oceans & Atmosphere - Hobart (hasAssociationWith) CSIRO Oceans & Atmosphere - IOMRC Crawley (hasAssociationWith) CSIRO Oceans & Atmosphere - St. Lucia (hasAssociationWith) Clementson, Lesley (pointOfContact) |
format |
Dataset |
title |
Southern Surveyor Voyage SS 11/95 HPLC Pigment and Ocean Colour Data |
title_short |
Southern Surveyor Voyage SS 11/95 HPLC Pigment and Ocean Colour Data |
title_full |
Southern Surveyor Voyage SS 11/95 HPLC Pigment and Ocean Colour Data |
title_fullStr |
Southern Surveyor Voyage SS 11/95 HPLC Pigment and Ocean Colour Data |
title_full_unstemmed |
Southern Surveyor Voyage SS 11/95 HPLC Pigment and Ocean Colour Data |
title_sort |
southern surveyor voyage ss 11/95 hplc pigment and ocean colour data |
publisher |
Integrated Marine Observing System |
url |
https://researchdata.edu.au/southern-surveyor-voyage-colour-data/476170 |
op_coverage |
Spatial: westlimit=139; southlimit=-53; eastlimit=152; northlimit=-40 Temporal: From 1995-11-15 to 1995-12-07 |
genre |
Southern Ocean |
genre_facet |
Southern Ocean |
op_source |
CSIRO Oceans & Atmosphere - Hobart |
op_relation |
https://researchdata.edu.au/southern-surveyor-voyage-colour-data/476170 a2683507-5abd-402b-a95e-8e42c47bcd7c Marlin Record Number: 6444 Anzlic Identifier: ANZCW0306006444 |
_version_ |
1810481827283992576 |